Transgenosis biocontrol engineering fungus for expressing stomach toxicity insecticidal protein by using endogenous security label and construction method of transgenosis biocontrol engineering fungus
A safety labeling, transgenic technology, applied in microorganism-based methods, biochemical equipment and methods, biocides, etc., can solve problems such as inability to grow normally, loss of function, blocked uracil synthesis, etc.
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[0021] The recombinant engineering strain obtained by the method of the present invention will be described in detail in combination with experiments below.
[0022] The wild strain Bb2860 of Beauveria bassiana, the ura3 knockout strain △ura3, and the engineering strains BbV28 and BbHV8 expressing Vip3Aa1 are all strains preserved or constructed in our laboratory. The construction of BbV28 used the common fungal promoter, PgpdA of Aspergillus nidulans, to drive the expression of vip3Aa1 in Bb2860, and the construction of BbHV8 used the optimized endogenous hydrophobin gene (hyd1) promoter Phydl-tl to drive the high expression of vip3Aa1 in Bb2860 , both screening markers are glufosinate resistance gene bar. Plasmids: pAN52-Phydl-tl-Vip3Aa1 and p0380-ura3, both of which were constructed in the previous research of our laboratory. The ELISA reagent TMBcomponent was purchased from Amresco (Solon, OH, USA), and the rabbit serum polyclonal antibody to Vip3Aa1 protein was provided ...
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