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Streptomyces avalmannii active spore insecticide and preparation method thereof

A technology of Streptomyces avermanii and active spores, applied in the direction of nematicides, botanical equipment and methods, biocides, etc., to achieve the effects of long-lasting temperature, improved control effect, and high insecticidal efficacy

Pending Publication Date: 2022-03-15
HEBEI XINGBAI AGRI SCI & TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, because the current biopesticide technology is still in its infancy, people only focus on the research and development of its metabolites of Streptomyces avermanii, and the research on its live spore products is still blank. Therefore, it is urgent to develop a kind of Avermansi. Streptomyces avermanii active spore insecticide and preparation method thereof improve the control effect on underground pests and promote the development of streptomyces avermanii active spore industry

Method used

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  • Streptomyces avalmannii active spore insecticide and preparation method thereof
  • Streptomyces avalmannii active spore insecticide and preparation method thereof
  • Streptomyces avalmannii active spore insecticide and preparation method thereof

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preparation example Construction

[0032] 1, the preparation method of Streptomyces avermanii active spore, comprises the steps:

[0033] 1. Culture medium formula:

[0034] Eggplant bottle slant medium, including the following raw materials in percentage by mass: beef extract 0.3%, peptone 0.5%, glucose 0.5%, potassium dihydrogen phosphate 0.05%, magnesium sulfate 0.03%, agar 2.0%, water 100mL, pH6. 8; Disinfect and sterilize with damp heat at 120 degrees Celsius for 30 minutes under a pressure of 0.11-0.12 MPa;

[0035] Solid fermentation medium: first compound the raw materials according to the following percentage mass ratio: 68.8% of rice bran, 13% of bran, 4% of bean cake, 4% of peanut cake powder, 9% of corn flour, 0.5% of calcium carbonate, and 0.5% of ammonium sulfate %, blood peptone 0.2%, pH natural, then add water to adjust the humidity to 55%; sterilize with damp heat at 120°C for 30min under a pressure of 0.11-0.12MPa;

[0036] 2. Fermentation process:

[0037] Step 1, the strain preparation of...

Embodiment 1

[0057] A kind of Streptomyces avermanii active spore insecticide, comprises each raw material of following weight:

[0058] Ammonium sulfate: 20kg;

[0059]Dextrin: 20kg;

[0060] Bentonite: 55kg;

[0061] Streptomyces avermanii active spore solid fermentation product: 15kg;

[0062] The content of active spores in the active spore solid fermentation product of Streptomyces avermanii is 5.03 billion / g;

[0063] In the present embodiment, insecticide weight is 110kg;

[0064] In this embodiment, the theoretical addition amount of the active spore solid fermentation product of Streptomyces avermanii is 685.91 million / g.

[0065]

[0066] A preparation method of Streptomyces avermanii active spore insecticidal granules, comprising the steps of:

[0067] Step 1. Weigh ammonium sulfate, dextrin, bentonite and active spore solid fermentation product of Streptomyces avermanii by weight parts, and mix evenly, and then pulverize until the particle size of the material reaches 1...

Embodiment 2

[0071] A kind of Streptomyces avermanii active spore insecticide, comprises each raw material of following weight:

[0072] Ammonium sulfate: 25kg;

[0073] Dextrin: 25kg;

[0074] Bentonite: 50kg;

[0075] Streptomyces avermanii active spore solid fermentation product: 13kg;

[0076] The content of active spores in the active spore solid fermentation product of Streptomyces avermanii is 5.03 billion / g;

[0077] In the present embodiment, insecticide weight is 113kg;

[0078] In this embodiment, the theoretical addition amount of the active spore solid fermentation product of Streptomyces avermanii is 578.67 million / g.

[0079]

[0080] A preparation method of Streptomyces avermanii active spore insecticidal granules, comprising the steps of:

[0081] Step 1. Weigh ammonium sulfate, dextrin, bentonite and Streptomyces avermanii active spore solid fermentation product in parts by weight, mix evenly, and then pulverize until the particle size of the material reaches 100 ...

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Abstract

The invention relates to a streptomyces avermimannii active spore insecticide and a preparation method thereof, and the streptomyces avermimannii active spore insecticide comprises the following raw materials by weight: 15-25 parts of ammonium sulfate; 15 to 25 parts of dextrin; 40 to 60 parts of bentonite; 0.5-1 billion of streptomyces avalmannii active spores are required to be added into each gram of the insecticide. The preparation method comprises the following steps: step 1, weighing ammonium sulfate, dextrin, bentonite and a streptomyces avermimannii active spore solid fermentation product in parts by weight, uniformly mixing, and then crushing until the material granularity reaches 100-200 meshes; and step 2, spraying water to the crushed material, extruding and granulating to obtain the streptomyces avalmannii active spore insecticidal granules. The insecticide disclosed by the invention has a good control effect on underground pests, especially pathogenic nematodes.

Description

technical field [0001] The invention belongs to the technical field of biological pesticides, and in particular relates to a Streptomyces avermanii active spore insecticide and a preparation method thereof. Background technique [0002] Streptomyces avermanii is a kind of actinomycete with insecticidal and acaricidal effects. Its live spores can parasitize the body and surface of larvae and eggs of various crop pathogenic nematodes and other underground pests, and reproduce in large numbers in the host body. Ultimately kill underground pests, and its metabolite antibiotics are effective ingredients for killing underground pests, with high safety and no pollution to the environment. However, because the current biopesticide technology is still in its infancy, people only focus on the research and development of its metabolites of Streptomyces avermanii, and the research on its live spore products is still blank. Therefore, it is urgent to develop a kind of Avermansi. The Str...

Claims

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Application Information

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IPC IPC(8): A01N63/28A01N25/12A01P5/00
CPCA01N63/28A01N25/12
Inventor 暴连群刘中须王炳太祁桂玲候慧云李巧颖
Owner HEBEI XINGBAI AGRI SCI & TECH CO LTD
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