Streptomyces avalmannii active spore insecticide and preparation method thereof

A technology of Streptomyces avermanii and active spores, applied in the direction of nematicides, botanical equipment and methods, biocides, etc., to achieve the effects of long-lasting temperature, improved control effect, and high insecticidal efficacy

Pending Publication Date: 2022-03-15
HEBEI XINGBAI AGRI SCI & TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, because the current biopesticide technology is still in its infancy, people only focus on the research and development of its metabolites of Streptomyces avermanii, and the research on its live spore products is still blank. Therefore, i

Method used

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  • Streptomyces avalmannii active spore insecticide and preparation method thereof
  • Streptomyces avalmannii active spore insecticide and preparation method thereof
  • Streptomyces avalmannii active spore insecticide and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0032] Process for the preparation of viable spores of Streptomyces Afo Man, comprising the steps of:

[0033] First, the medium formula:

[0034] Eggplant flask slant medium, including the percentage of the mass ratio of raw materials as follows: beef extract 0.3%, 0.5% peptone, 0.5% glucose, 0.05% potassium dihydrogen phosphate, 0.03% magnesium sulfate, 2.0% agar, water 100mL, pH6. 8; 0.11-0.12MPa pressure at 120 degrees Celsius for 30 min moist heat sterilization;

[0035] Solid fermentation medium: press as percentage mass ratio of each raw material compound: 68.8% bran, wheat bran 13%, 4% soybean meal, peanut meal 4%, 9% corn meal, 0.5% calcium carbonate, ammonium sulfate 0.5 %, blood peptone 0.2%, pH natural, then water was added to adjust the humidity of 55%; 0.11-0.12MPa pressure at 120 degrees Celsius for 30 min moist heat sterilization;

[0036] Second, the fermentation process:

[0037] Step 1, Streptomyces sp prepared Afo Man:

[0038] The cryopreserved Afo Man Strepto...

Example Embodiment

[0056] Example 1:

[0057] One kind Afo Man insecticides viable spores of Streptomyces, including the following raw materials by weight:

[0058] Ammonium sulfate: 20kg;

[0059]Fastult: 20kg;

[0060] Bentonite: 55kg;

[0061] Avoremoman streptoma active spore solid fermentation product: 15kg;

[0062] The content of active spores in Affman streptoma active spore solid fermenter is 5033 million / gram;

[0063] In this embodiment, the insecticide weight is 110 kg;

[0064] In this example, the theoretical addition of the Affman streptoma active spore solid fermentation product is 6859.1 billion / gram.

[0065]

[0066] A method of preparation of Affman streptoma active spore insecticidal granules, including the following steps:

[0067] Step 1, after the weight of the ammonium sulfate, dextrin, bentonite, and Afman streptoma active spore solids fermentation product, and mix well, then pulverized to material particle size of 150;

[0068] Step 2, the pulverized material water i...

Example Embodiment

[0070] Example 2

[0071] A Avo Differencetomycin active spore pesticide, including the following weights:

[0072] Ammonium sulfate: 25kg;

[0073] Fiction: 25kg;

[0074] Bentonite: 50kg;

[0075] Afigon streptoma active spore solid fermentation product: 13kg;

[0076] The content of active spores in Affman streptoma active spore solid fermenter is 5033 million / gram;

[0077] In this embodiment, the insecticide weight is 113 kg;

[0078] In this example, the theoretical amount of the Affman streptoma active spore solid fermentation product is 5786.7 billion / gram.

[0079]

[0080] A method of preparation of Affman streptoma active spore insecticidal granules, including the following steps:

[0081] Step 1, after weighing the ammonium sulfate, dextrin, bentonite, and Afman streptoma active spore solid fermentation product, and mix uniform, then pulverized to material particle size of 100 mesh;

[0082] Step 2, the pulverized material water is extruded to the granulation, an...

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Abstract

The invention relates to a streptomyces avermimannii active spore insecticide and a preparation method thereof, and the streptomyces avermimannii active spore insecticide comprises the following raw materials by weight: 15-25 parts of ammonium sulfate; 15 to 25 parts of dextrin; 40 to 60 parts of bentonite; 0.5-1 billion of streptomyces avalmannii active spores are required to be added into each gram of the insecticide. The preparation method comprises the following steps: step 1, weighing ammonium sulfate, dextrin, bentonite and a streptomyces avermimannii active spore solid fermentation product in parts by weight, uniformly mixing, and then crushing until the material granularity reaches 100-200 meshes; and step 2, spraying water to the crushed material, extruding and granulating to obtain the streptomyces avalmannii active spore insecticidal granules. The insecticide disclosed by the invention has a good control effect on underground pests, especially pathogenic nematodes.

Description

technical field [0001] The invention belongs to the technical field of biological pesticides, and in particular relates to a Streptomyces avermanii active spore insecticide and a preparation method thereof. Background technique [0002] Streptomyces avermanii is a kind of actinomycete with insecticidal and acaricidal effects. Its live spores can parasitize the body and surface of larvae and eggs of various crop pathogenic nematodes and other underground pests, and reproduce in large numbers in the host body. Ultimately kill underground pests, and its metabolite antibiotics are effective ingredients for killing underground pests, with high safety and no pollution to the environment. However, because the current biopesticide technology is still in its infancy, people only focus on the research and development of its metabolites of Streptomyces avermanii, and the research on its live spore products is still blank. Therefore, it is urgent to develop a kind of Avermansi. The Str...

Claims

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Application Information

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IPC IPC(8): A01N63/28A01N25/12A01P5/00
CPCA01N63/28A01N25/12
Inventor 暴连群刘中须王炳太祁桂玲候慧云李巧颖
Owner HEBEI XINGBAI AGRI SCI & TECH CO LTD
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