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A strain of fritillaria ussuriensis maxim rhizosphere Paenibacillus polymyxa, and applications thereof,

A technology of Paenoid polymyxa and Fritillaria, applied in the direction of bacteria, fungicides, chemicals for biological control, etc., can solve the problems of destroying the soil micro-ecological environment and high residual harmful substances, and improve the soil micro-ecological environment , good control effect

Active Publication Date: 2017-12-22
HEILONGJIANG PROV FOREST PROTECTION INST
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Problems solved by technology

Because the pathogenic bacteria that harm the underground bulbs of Fritillaria are soil-borne pathogenic bacteria, they have good facultative parasitic ability in the soil and strong rot survival ability. The growth conditions of the Fritillaria are closely related to each other. Studies have proved that the disorder of the soil micro-ecological environment and the destruction of soil physical and chemical properties are the main reasons for the continuous occurrence of black rot. Therefore, it is difficult to achieve the ideal control effect by taking agricultural measures, and it is difficult to fundamentally solve the problem. The problem is that the long-term excessive use of chemical pesticides such as soil disinfectants will also damage the soil micro-ecological environment, and at the same time cause excessive residues of harmful substances in the bulbs of Fritillaria. Therefore, an environmental harmony method is urgently needed to continuously control the black rot of Fritillaria. occurrence of disease

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  • A strain of fritillaria ussuriensis maxim rhizosphere Paenibacillus polymyxa, and applications thereof,

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specific Embodiment approach 1

[0014] Specific embodiment one: present embodiment flat fritillaria rhizosphere Paenibacillus polymyxa is Paenibacillus polymyxa ( Paenibacillus polymyxa ) SBS-SF0105, deposited in the General Microbiology Center (CGMCC) of the China Committee for the Collection of Microbial Cultures (CGMCC), the preservation address is No. 3, No. 1, Beichen West Road, Chaoyang District, Beijing, Institute of Microbiology, Chinese Academy of Sciences, and the preservation date is November 2015 On the 5th, the deposit number is CGMCC No.11586.

[0015] In this embodiment, Paenibacillus polymyxa SBS-SF0105 is cultured at 25° C. for 48 hours on PDA medium, and the colony becomes colorless and transparent without pigmentation. The diameter of the colony is 1.5-2.0 mm, and the colony is moist and sticky with neat edges. The bacterial cell is rod-shaped, the spore is oval, the cyst is enlarged, and the Gram stain is positive.

[0016] Paenibacillus polymyxa (Paenibacillus polymyxa) Paenibacillus...

specific Embodiment approach 2

[0018] Specific embodiment 2: In this embodiment, Paenibacillus polymyxa SBS-SF0105 is isolated from the rhizosphere of Pygnus chinensis. The separation method is carried out as follows:

[0019] 1) Isolation and preservation of rhizosphere bacteria of Fritillaria chinensis: In the cultivation plots of Pingbei, take healthy Pingbei plants, and collect rhizosphere soil samples by shaking off method. First, gently shake off the soil without roots, and then use a brush The rhizosphere soil on the roots and bulbs was brushed down and collected. Take 1g of rhizosphere soil and put it into a 250ml sterilized triangular flask, add 100ml of sterile water, 30-40 sterilized glass beads, shake at 160rpm on a shaker for 30min, let it stand for 30min, take the supernatant, and dilute the concentration by gradient dilution method to 10 -4 、10 -5 、10 -6 For three gradients, draw 0.1ml of liquid from the three gradients and inoculate them on beef extract peptone solid medium, culture at a...

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Abstract

The invention provides a strain of fritillaria ussuriensis maxim rhizosphere Paenibacillus polymyxa, and applications thereof, relates to a strain of Paenibacillus polymyxa and applications, and provides fritillaria ussuriensis maxim rhizosphere Paenibacillus polymyxa. According to the present invention, the Paenibacillus polymyxa SBS-SF0105 is preserved in the China General Microbiological Culture Collection Center on November 5, 2015, and has the preservation number of CGMCC No.11586; the strain has characteristics of wide antibacterial spectrum and strong antibacterial ability, can be colonized at the rhizosphere of fritillaria ussuriensis maxim and other plants, and can be used for preventing and controlling soil-borne diseases of fritillaria ussuriensis maxim, schisandra chinensis, acanthopanax senticosus and other plants; and the biological bacterial agent prepared by using the strain as the main component can effectively solve the succession cropping obstacle problem of fritillaria ussuriensis maxim.

Description

technical field [0001] The invention relates to a strain of Paenibacillus polymyxa and its application. Background technique [0002] Flat Fritillary ( Fritillaria ussuriensis Maxim) is a perennial herb of the genus Fritillaria in the family Liliaceae, and is one of the important medicinal materials in Northeast China. The active ingredient of flat shell bulbs is alkaloids. It has the effects of clearing away heat and moistening the lungs, reducing phlegm and relieving cough, dispelling stagnation and reducing swelling, and is used to treat various diseases such as bronchitis, pneumonia, and whooping cough. Flat Fritillaria is not only a bulk medicinal material, but also a raw material for medicine, health care and other industries. Heilongjiang Province is the main cultivation area of ​​Fritillaria fritillata. With the complete cessation of commercial logging and the transformation of business models in Heilongjiang Forest Industry, the under-forest economy has become an ...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01P3/00C12R1/12
CPCC12N1/205C12R2001/12
Inventor 宋小双邓勋遇文婧周琦王琪高宇
Owner HEILONGJIANG PROV FOREST PROTECTION INST
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