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Fenugreek extract and preparation method thereof, pharmaceutical composition containing fenugreek extract and use of pharmaceutical composition

A technology of fenugreek and extract, which is applied in the field of preparation of fenugreek extract, can solve the problems of difficult separation of organic solvents and the like

Inactive Publication Date: 2018-12-07
PRINCE PHARMA CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, as modern people pay more attention to food safety, since organic solvents are easy to remain in the extract and difficult to separate, the extraction method needs to be further improved

Method used

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  • Fenugreek extract and preparation method thereof, pharmaceutical composition containing fenugreek extract and use of pharmaceutical composition
  • Fenugreek extract and preparation method thereof, pharmaceutical composition containing fenugreek extract and use of pharmaceutical composition
  • Fenugreek extract and preparation method thereof, pharmaceutical composition containing fenugreek extract and use of pharmaceutical composition

Examples

Experimental program
Comparison scheme
Effect test

preparation example 1

[0045] The water extraction method of preparation example 1 Fenugreek

[0046] Fresh or dried fenugreek seeds are extracted with a water solvent, wherein the weight ratio of the water solvent used is 1 to 20 times that of the fenugreek seeds, preferably 5 to 10 times. The fenugreek seeds soaked in the water solvent are placed at 25°C to 100°C for 0.5 hours to 5 hours, preferably 50°C to 95°C for 0.5 hours to 3 hours, and filtered (by a known physical filtration method) After solid-liquid separation, such as porosity, weight or density), fenugreek extract (abbreviated as YE2) is obtained. The fenugreek extract can be concentrated or dried by known food processing methods.

preparation example 2

[0047] The organic solvent extraction method of preparation example 2 Fenugreek

[0048] Fresh or dried fenugreek seeds are extracted with 50% alcohol solvent, wherein the weight ratio of the alcohol solvent used is 1 to 20 times, preferably 5 to 10 times, that of the fenugreek seeds. Place the fenugreek seeds soaked in alcohol solvent at 25°C to 100°C for 0.5 hours to 5 hours, preferably at 50°C to 95°C for 0.5 hours to 3 hours, and filter (by a known physical filtration method) After solid-liquid separation, such as porosity, weight or density), an alcoholic extract of fenugreek (abbreviated as YE3) is obtained. The fenugreek extract can be concentrated or dried by known food processing methods.

Embodiment 1

[0049] Example 1 Cell Experiment - Dosing and Stimulation Simultaneously, Simultaneously Preventing Mechanism

[0050] (1) Liver hepatocellular carcinoma (HepG2) was planted in each well at 2.5×10 4 cells were cultured for 24 hours.

[0051] (2) It is divided into the following groups:

[0052] Control group: add cell culture medium equal to the volume of the oil droplet inducer and 1% dimethylsulfoxide (DMSO) to the liver cancer cells for 6 hours.

[0053] Oil drop induction group: add 1% DMSO and 500 millimolar concentration (mM) of oil drop inducer [unit fatty acid oleic acid (oleic acid, OA, C18: 1) and saturated fatty acid palmitic acid (paltimic acid, PA, C16: 0), dissolved in cell culture medium at a volume ratio of 2:1 (v / v)] and stimulated in liver cancer cells for 6 hours.

[0054] Positive control group: add phosphoatidylcholine (phosphoatidylcholine) 0.01 microgram per milliliter (μg / mL) and oil drop inducer with 500 millimolar concentration (mM) to stimulate li...

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PUM

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Abstract

The invention provides a fenugreek extract and a preparation method thereof, a pharmaceutical composition containing the fenugreek extract and a use of the pharmaceutical composition. The preparationmethod of the fenugreek extract comprises the following steps: (1) preparing fenugreek plant tissues completely, soaking in an aqueous solvent, and extracting at the temperature of 25 DEG C to 100 DEGC for 0.5 h to 5 h; and (2) filtering the extracted fenugreek plant tissue to obtain the fenugreek extract. The invention also provides the composition containing the fenugreek extract. The inventionfurther provides the pharmaceutical composition containing the composition and a pharmaceutically acceptable carrier, and the use of the pharmaceutical composition. The fenugreek extract extracted bythe preparation method can effectively prevent or treat non-alcoholic fatty liver.

Description

technical field [0001] The invention relates to a preparation method of fenugreek extract, in particular to a preparation method of water-extracted fenugreek extract. The present invention also relates to a composition for preventing or treating non-alcoholic fatty liver, especially the fenugreek extract and red yeast extract. The present invention also relates to a pharmaceutical composition, particularly the aforementioned fenugreek extract, red yeast rice extract, rice bran, artichoke, ginseng or a combination thereof. The present invention also relates to the use of a pharmaceutical composition, in particular to the use of the aforementioned pharmaceutical composition for preventing or treating non-alcoholic fatty liver. Background technique [0002] Fatty liver is caused by accumulation of fat in liver cells. When the dietary intake of fat is more than the body can handle, the liver will gradually accumulate adipose tissue. If fat accounts for more than 5% of the liv...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/48A61K36/899A61P1/16A61K31/185
CPCA61K31/185A61K36/062A61K36/258A61K36/28A61K36/48A61K36/899A61K2236/19A61K2236/331A61K2300/00
Inventor 沈思妤
Owner PRINCE PHARMA CO LTD
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