Folium apocyni veneti total flavonoid near infrared super rapid detection method

A detection method, a technology of total flavonoids, applied in measuring devices, material analysis through optical means, instruments, etc., can solve the problems of cumbersome process, high cost of operation and maintenance, harmful to the health of operators, etc., and achieve high prediction accuracy , shorten the time and cost of detection, super fast effect

Inactive Publication Date: 2016-07-20
乌鲁木齐华新分析测试高科技开发公司
View PDF3 Cites 7 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The above methods all require pre-treatment of Apocynum leaf, that is, dissolving and extracting with organic solvents such as methanol, and the pre-treatment time needs at least 8 hours, and the chemicals in the operation are harmful to the health of the operator and pollute the environment
Not only are they all destructive testing, but also the process is cumbersome, time-consuming and labor-intensive, and the cost of operation and maintenance is high, which is not conducive to the rapid detection of the total flavonoid content of apocynum leaves

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Folium apocyni veneti total flavonoid near infrared super rapid detection method
  • Folium apocyni veneti total flavonoid near infrared super rapid detection method
  • Folium apocyni veneti total flavonoid near infrared super rapid detection method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Determination of chemical value: according to the "Determination of total flavonoids in health food" method in "Technical Specifications for Inspection and Evaluation of Health Food" (2002). Take 64 parts of apocynum leaves and crush them through a 40-mesh sieve, weigh 1.00 g of each part, add ethanol to make up to 25 mL, shake well, ultrasonically extract for 20 minutes, place it, absorb 1.0 mL of the supernatant, put it in an evaporating dish, Add 1g of polyamide powder for adsorption, evaporate ethanol on a water bath, and then transfer to a chromatographic column. First wash with 20mL of benzene, discard the benzene liquid, then elute the flavonoids with methanol, and dilute to 25mL. The absorption value of this solution was measured at a wavelength of 360nm. At the same time, with rutin as the standard, the standard curve was determined, the regression equation was obtained, and the total flavonoid content in the sample was calculated.

[0025] And make the rutin...

Embodiment 2

[0048] Determination of chemical value: 100 parts of apocynum leaves were crushed through a 60-mesh sieve, accurately weighed 1.00g, added ethanol to a volume of 25mL, shaken well, ultrasonically extracted for 20min, left to stand, and 1.0mL of the supernatant was drawn, and placed in an evaporating dish Add 1g of polyamide powder for adsorption, evaporate ethanol on a water bath, and then transfer to a chromatographic column. First wash with 20mL of benzene, discard the benzene liquid, then elute the flavonoids with methanol, dilute to 25mL, measure the absorption value of this liquid at a wavelength of 380nm; at the same time, use rutin as a standard, measure the standard curve, find the regression equation, and calculate the test value. The total flavonoid content in the samples.

[0049] Raw spectrum acquisition: 100 Apocynum leaf samples were crushed and passed through a 40-mesh standard sieve, numbered 1 to 100 respectively, using a rotating diffuse reflectance sampling ...

Embodiment 3

[0058] Determination of chemical value: take 77 parts of apocynum leaves and crush them through a 40-mesh sieve, accurately weigh 1.00g of each part, add ethanol to the volume to 25mL, shake well, ultrasonically extract for 20min, place it, and absorb 1.0mL of the supernatant , In an evaporating dish, add 1g polyamide powder for adsorption, evaporate ethanol on a water bath, and then transfer to a chromatography column. First wash with 20mL of benzene, discard the benzene liquid, then elute the flavonoids with methanol, dilute to 25mL, measure the absorption value of this liquid at a wavelength of 340nm; at the same time, use rutin as a standard, measure the standard curve, find the regression equation, and calculate the test value. The total flavonoid content in the samples.

[0059] Raw spectrum acquisition: 77 samples of Apocynum apocynum leaves were crushed and passed through a 40-mesh standard sieve, numbered 1-77 respectively, and the rotating diffuse reflectance samplin...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The present invention discloses a folium apocyni veneti total flavonoid near infrared super rapid detection method including chemical value measurement, original spectrum acquisition, spectral data preprocessing, PLS quantitative calibration model establishment, quantitative calibration model stability prediction, folium apocyni veneti total flavonoid mode content model prediction and other steps. Compared with the prior art, waste time and energy, higher operating and maintenance cost and other problems of folium apocyni veneti total flavonoid detection methods in the prior art can be fundamentally solved, a PLS quantitative model is established by use of near infrared spectroscopy for super rapid detection without sample pretreatment, the folium apocyni veneti total flavonoid near infrared super rapid detection method is convenient, fast, free of side effects on the human body and the environment and small in testing result relative deviation, and if a test sample chemical measured value has high precision, a predicted value of the method may be close to a true value.

Description

technical field [0001] The invention relates to the technical field of health food detection, in particular to a near-infrared ultra-fast detection method for total flavonoids in apocynum leaves. Background technique [0002] Apocynum apocynum leaf is the dried leaf of apocynum apocynum, which grows in Xinjiang, Dunhuang, Liaoning, Anhui and other places. It is one of the widely used traditional Chinese medicines. Total flavonoids is one of the important indicators to evaluate the quality of Apocynum apocynum leaves, because flavonoids are a class of natural antioxidants, which can improve the antioxidant capacity of the human body, and have various effects such as lowering blood lipids, diuresis, and anti-aging. Apocynum apocynum leaves contain aloe Ding, hyperoside, trefoil bean, kaempferol, astragalus bean glucoside and other flavonoids, the content of rutin in Xinjiang floret apocynum is relatively high. Determination of the total flavonoids content of Apocynum apocynum...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/359
Inventor 阿依古丽·塔什波拉提李暮春方智三
Owner 乌鲁木齐华新分析测试高科技开发公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap