Microfluidic chip based on sers detection, preparation method and application thereof

A technology of microfluidic chips and substrates, which is applied to chemical instruments and methods, measuring devices, and laboratory containers, etc. It can solve the problems of large loss when liquid flows, insufficient detection accuracy, and cumbersome preparation process, etc., and achieves an improvement. The effect of sensitivity

Active Publication Date: 2017-12-05
ZHEJIANG UNIV OF TECH +1
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0007] Aiming at the problems that the current microfluidic surface-enhanced Raman test chip has complex structure, complicated preparation process, large loss during liquid flow, uneven distribution of detected particles, and insufficient detection accuracy, the present invention proposes a simple structure Microfluidic chip based on SERS detection, preparation method and application thereof, which are easy to manufacture, have low liquid flow loss, uniform distribution of detected particles, and high detection accuracy

Method used

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  • Microfluidic chip based on sers detection, preparation method and application thereof
  • Microfluidic chip based on sers detection, preparation method and application thereof
  • Microfluidic chip based on sers detection, preparation method and application thereof

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Experimental program
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Effect test

Embodiment 1

[0033] Embodiment 1 The preparation method of the microfluidic chip based on SERS detection according to the present invention comprises the following steps:

[0034] 1) Preparation of superhydrophobic titanium dioxide coating solution: 0.1 g of titanium dioxide powder (P 25 ) was mixed with 10mL of absolute ethanol, and then ultrasonicated at 100Hz for 30min to form a 0.01g / mL titanium dioxide suspension, then 0.2mL of octadecyltrimethoxysilane was added, and then ultrasonically mixed at 100Hz for 10min. React for 12 hours to obtain a superhydrophobic titanium dioxide coating solution; the volume ratio of the silane to the titanium dioxide suspension is 0.02:1;

[0035] 2) Preparation of a substrate with a superhydrophobic layer: use a spin coater to spin-coat the superhydrophobic titanium dioxide coating solution onto the cleaned upper surface of the substrate, and then place it in an oven for 1 hour at 100°C to obtain a substrate with a superhydrophobic layer , the contact...

Embodiment 2

[0043] Example 2 The microfluidic chip based on SERS detection prepared by the preparation method of the present invention includes a substrate 1 and a T-shaped flow channel 2 arranged on the surface of the substrate, and the two ends of the T-shaped flow channel 2 are As two flow channel inlets (21,22), the end of the vertical flow channel 23 of the T-shaped flow channel 2 is used as the detection area 231; Each region is evenly distributed with hydrophobic patterns 24, and each hydrophobic pattern 24 corresponds to the corresponding position of the substrate as a superhydrophobic region, and the part outside the hydrophobic pattern is a superhydrophilic region; The number of hydrophobic patterns in the area is decreasing, that is, the distance between adjacent hydrophobic patterns is gradually increasing, that is, the proportion of the corresponding hydrophilic area on the substrate 1 is gradually increasing; the end of the vertical channel 23 is provided with a detection are...

Embodiment 3

[0045] The application of the micro-control flow chip described in Example 3 in the simultaneous detection of liquid analytes is characterized in that: the application method is carried out according to the following steps:

[0046] (1) configuration of Rhindan B solution and a concentration of Au nanoparticles solution of 0.5mM;

[0047] (2) Drop 5 μL of Au nanoparticle solution at the inlet of one of the flow channels of the T-shaped flow channel, and at the same time, drop 5 μL of rhinidine B solution at the inlet of the other flow channel;

[0048] (3) Since two wetting gradients are set in the cross flow channel, it can provide a driving force for the movement of the droplet and realize the self-driving of the droplet. The two droplets merge at the intersection of the cross flow channel and move after being fully mixed in the vertical flow channel. Go to the designated detection area at the end of the vertical flow channel, and perform in-situ Raman detection in the detec...

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Abstract

The invention relates to a micro-fluidic chip based on SERS detection, a preparation method and an application thereof. The preparation method comprises the steps of preparing a super-hydrophobic titanium dioxide solution, preparing a substrate with a super-hydrophobic layer and preparing a chip, wherein the chip comprises the substrate and a T-shaped runner arranged on the surface of the substrate; the interior of the runner is super-hydrophilic and the exterior of the runner is super-hydrophobic; two ends of a cross runner of the T-shaped runner are used as two runner inlets; a detection area is arranged at the tail end of a vertical runner; super-hydrophobic micro-patterns are distributed in the cross runner of the T-shaped runner; the micro-patterns are divided into a plurality of areas from two ends to the center; the intervals of the super-hydrophobic patterns in each area are equal to each other; the pattern interval gradient between area and area along the direction from two ends to the center is increased; a wetting gradient from lyophobic state to hydrophilic state is formed in the cross runner from two ends to the center; the vertical runner is designed as a wedge-shaped runner. The micro-fluidic chip based on SERS detection has the beneficial effects that the surface wetting gradient and the wedge-shaped structure runner are combined for realizing the automatic transportation of drop to the detection area and the cost of micro-fluid driving is greatly reduced.

Description

technical field [0001] The invention relates to a microfluidic chip based on SERS detection, a preparation method and an application thereof. Background technique [0002] Surface Enhanced Raman Scattering (SERS) refers to the technology of enhancing the Raman signal of adsorbed molecules with the help of rough metal surface or metal nanostructure, which can enhance the Raman signal by 1010 to 1011 times, which means that it Single molecules can be detected. Due to its ultra-high sensitivity, SERS technology has been widely used in chemistry, biology, medicine and other fields. [0003] The microfluidic chip is a micron-level channel made by micro-machining. Through the design of the channel, various basic processes of analysis, such as sample pretreatment, separation, analysis and detection, are integrated on a small substrate to realize the analysis of proteins, inorganic The rapid calibration and detection of chemical components such as ions and DNA are called microflui...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): B01L3/00G01N21/65
CPCB01L3/5027B01L3/502707B01L2200/10G01N21/658
Inventor 吴化平刘爱萍李吉泉丁浩柴国钟朱凯曹彬彬吴兵兵
Owner ZHEJIANG UNIV OF TECH
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