Serum-free culture medium for umbilical cord blood mesenchymal stem cells

A technology of serum-free medium and mesenchymal stem cells, which is applied in the field of serum-free medium of umbilical cord blood mesenchymal stem cells, to achieve the effect of improving activity, avoiding immune rejection, and reducing cell apoptosis rate

Inactive Publication Date: 2016-08-24
GUANGDONG PANGUARD CELL BIOLOGICAL TECH CO LTD
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  • Application Information

AI Technical Summary

Problems solved by technology

Interleukin 3 (Interleukin3IL-3) is beneficial to the growth and activity maintenance of umbilical cord blood stem cells, but it has an inhibitory effect on the early proliferation and differentiation of umbilical cord blood mesenchymal stem cells

Method used

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  • Serum-free culture medium for umbilical cord blood mesenchymal stem cells
  • Serum-free culture medium for umbilical cord blood mesenchymal stem cells
  • Serum-free culture medium for umbilical cord blood mesenchymal stem cells

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Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0031] Prepare high-glucose α-MEM culture medium according to Table 1

[0032] Table 1 High-sugar α-MEM formula table

[0033]

[0034]

[0035] a. Prepare umbilical cord blood mesenchymal stem cell culture medium: add insulin, transferrin, BMP-4, BOP1, FGF-2 and AMD3100 according to the concentration in α-MEM culture medium to make umbilical cord blood mesenchymal stem cell culture medium;

[0036] b. Addition of cytokines: the experiment was divided into five groups, the first group was the control group without IL-3, the second group was the control group without FL, and the third group was four kinds of cytokines added at the same time, in no particular order Sequentially, the fourth group is the method for culturing umbilical cord blood mesenchymal stem cells of the present invention, and the fifth group is a blank control experiment in which none of the four cytokines is added. The details are as follows:

[0037] The first group IL-6+SCF+FL

[0038] The second g...

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Abstract

The invention aims at providing a serum-free culture medium for umbilical cord blood mesenchymal stem cells. The serum-free culture medium is composed of a cell factor combination, an alpha-MEM culture solution, insulin, transferring, BMP-4, BOP1, FGF-2 and AMD3100. The cell factor combination comprises IL-3, IL-6, SCF and FL, the concentration of IL-3 is 1-20 ng / ml, the concentration of IL-6 is 1-100 ng / ml, the concentration of SCF is 1-100 ng / ml, and the concentration of FL is 1-100 ng / ml. The serum-free culture medium is developed and researched, the adding sequence of hematopoiesis factors is changed, and the defects of existing umbilical cord blood mesenchymal stem cell culture media in the prior art are overcome.

Description

technical field [0001] The invention relates to the field of biology, in particular to a serum-free medium for umbilical cord blood mesenchymal stem cells. Background technique [0002] Umbilical cord blood mesenchymal stem cells (UCB-MSCs) have good differentiation potential and have broad clinical prospects in tissue engineering such as bone, cartilage, muscle, nerve, liver, and myocardium. Umbilical cord blood mesenchymal stem cells can be isolated from human umbilical cord, which has the advantages of convenient sampling and no ethical controversy. Umbilical cord blood mesenchymal stem cells have a strong immune regulation effect, can promote hematopoietic recovery function and repair diseased tissues and organs, and have a high clinical value in organ transplantation, autoimmune diseases, leukemia, bone and muscle degenerative diseases, etc. Value. [0003] How to achieve rapid expansion of umbilical cord blood mesenchymal stem cells without affecting their function i...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/0775
CPCC12N5/0665C12N2500/24C12N2500/90C12N2501/115C12N2501/125C12N2501/155C12N2501/2303C12N2501/2306C12N2501/26C12N2501/33
Inventor 谢海涛李相鲁张严冬
Owner GUANGDONG PANGUARD CELL BIOLOGICAL TECH CO LTD
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