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Geocoris pallidipennis vitellogenin new gene and application thereof

A vitellogenin and eye bug technology, which is applied in the fields of genetic engineering and protein engineering, can solve the problems of Ptera, Hemiptera, Lepidoptera, Diptera, Coleoptera, Neuroptera and Hymenoptera, large There is no report on the cloning of the Vg gene of the stink bug

Inactive Publication Date: 2016-08-24
INST OF PLANT PROTECTION CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, the Vg genes of various insects have been cloned and identified, mainly including insects of Diptera, Hemiptera, Lepidoptera, Diptera, Coleoptera, Neuroptera and Hymenoptera. The cloning of Vg gene of Chrysanthemum chinensis has not been reported

Method used

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  • Geocoris pallidipennis vitellogenin new gene and application thereof
  • Geocoris pallidipennis vitellogenin new gene and application thereof
  • Geocoris pallidipennis vitellogenin new gene and application thereof

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example 1

[0014] The cloning of the cDNA of the vitellogenin Vg gene of Chrysoma macrophthalmia was carried out through the following steps: total RNA was extracted from the eclosion female adults of Chrysanthemum macrophthalmia; Design two pairs of degenerate primers for the Vg conserved region, use the above cDNA as a template for PCR amplification, purify the PCR product obtained in the above steps, recover the target fragment from agarose gel, take the purified product and connect it to the pMD19-T simple vector, and then transform Escherichia coli competent cell DH5a was cultured on a plate at 37°C, and positive clones were detected and identified by PCR with M13 universal primers; according to the results of clone sequencing, specific primers were designed to connect the sequences between the two fragments; according to Clontech-RACE reagent The kit operation steps reverse transcription to synthesize the first strand of cDNA, and design 3' and 5'-RACE specific primers according to ...

example 2

[0016] The DNA sequence of the vitellogenin gene GpVg of Geocoris pallidipennis obtained above is SEQ ID NO: 1, the encoded protein contains 1848 amino acids, and its sequence is shown in SEQ ID NO: 2. Compared in the database, the protein encoded by the vitellogenin gene GpVg has 57%, 43%, 40%, and 43% similarity with Riptortus clavatus, Plautia stali, Triatomainfestans, and Pyrrhocoris apterus in the database, and Apolygus lucorum, Nesidiocoris tenuis, etc. Both are below 40%.

[0017] To sum up, the present invention discloses a vitellogenin Vg gene (GpVg) of Chrysanthemum macrophthalmia and its encoded protein. This gene is the first report in the Hemiptera natural enemy insect, Chrysanthemum chinensis. The present invention proves that the gene can be applied to the study of the nutritional, physiological and reproductive characteristics of natural enemy insects, and provides theoretical guidance for the large-scale breeding and effective utilization of this important nat...

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Abstract

The invention discloses an important hemiptera natural enemy insect geocoris pallidipennis vitellogenin gene DNA sequence and an encoded protein sequence; hemiptera insect vitellogenin gene molecular characteristic information is complemented, and the new gene is provided for study on an action mechanism of the vitellogenin gene in geocoris pallidipennis nutrition and reproductive regulation and vitellogenin gene functions. A theoretical basis is provided for geocoris pallidipennis mass propagation and effective use, and broad prospects for application in biological prevention and treatment of injurious insects are achieved.

Description

technical field [0001] The invention discloses a new vitellogenin gene DNA sequence and its coded protein sequence, belonging to the fields of genetic engineering and protein engineering. The invention relates to the cloning and application of a new vitellogenin gene of Geocoris pallidipennis. Background technique [0002] Vitellogenin (Vg) is a protein that specifically exists in the blood of non-mammalian sexually mature oviparous female animals, and is the precursor of vitellogenin in almost all oviparous animals. Vitellogenin is a key substance for vitellogenesis and plays an important role in the reproductive development of oviparous animals. It is synthesized in the female fat body, secreted into the hemolymph, and then taken up by the developing oocyte through endocytosis. The developing embryo provides nutrients and functional substances such as amino acids, fats, carbohydrates, vitamins, phosphorus, sulfur and trace elements. [0003] The study of insect vitelloge...

Claims

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Application Information

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IPC IPC(8): C12N15/12C07K14/435C12N15/10
Inventor 曾凡荣梁慧芳毛建军
Owner INST OF PLANT PROTECTION CHINESE ACAD OF AGRI SCI
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