Primer, probe and kit for evaluating sun protection condition of skin

A kit and skin technology, applied in the field of cosmetology, can solve problems such as difficult to achieve personalized maintenance or treatment, and achieve the effect of clear molecular mechanism, strong specificity and high sensitivity

Inactive Publication Date: 2016-08-24
FUJIAN AIWO HEALTH BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The above patents/patent applications all use foreign substances to regulate the expression of genes in the body, but there

Method used

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  • Primer, probe and kit for evaluating sun protection condition of skin
  • Primer, probe and kit for evaluating sun protection condition of skin
  • Primer, probe and kit for evaluating sun protection condition of skin

Examples

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Embodiment 1

[0076] Example 1: Primer and probe design

[0077] 1. Design and synthesize the following primers and probes. Synthesis unit: Applied Biosystems, USA Applied Biotechnology Co., Ltd.

[0078] MC1R (rs2228479) primer and fluorescent probe sequence:

[0079] Forward primer CACCATCGCCAAGAACCGGAA SEQ ID NO: 1;

[0080] Reverse primer AGCTGCAGGTGATCACGTC SEQ ID NO: 2;

[0081] Fluorescent probe FAM-AGCAAC A TGCTGGAGACGGCC-MGB SEQ ID NO: 3;

[0082] Fluorescent probe VIC-AGCAAC G TGCTGGAGACGGCC-MGB SEQ ID NO: 4;

[0083] MC1R (rs885479) primer and fluorescent probe sequence:

[0084] Forward primer ACATCTCCATCTTCTACGCACT SEQ ID NO:5;

[0085] Reverse primer CCATGAGCACCAGCATAGCC SEQ ID NO: 6;

[0086] Fluorescent probe FAM-CCTGCCGCGGGCGCGGC A AGCCG-MGB SEQ ID NO: 7;

[0087] Fluorescent probe VIC-CCTGCCGCGGGCGCGGC G AGCCG-MGB SEQ ID NO: 8;

[0088] ASIP (rs1015362) primer and fluorescent probe sequence:

[0089] Forward primer CAGGTAGAATGTACTAACCCCT SEQ ID NO: 9;

[0090] ...

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Abstract

The invention discloses a primer, probe and kit for evaluating the sun protection condition of skin. The sequences of the primer and the probe are SEQ ID NO:1-4, SED ID NO:5-8, SEQ ID NO:9-12 and SEQ ID NO:13-16, wherein the 5' ends and the 3'ends of SEQ ID NO:3-4, SEQ ID NO:7-8, SEQ ID NO:11-12 and SEQ ID NO:15-16 are connected with different fluorescence markers respectively. The invention further discloses a method for assisting a user in selecting a correct beautifying method through gene detection. The internal cause and the external cause of sunburn, freckles and pigment accumulation of skin are leant, a user-friendly beautifying healthy solution is provided, and the effects of sun protection beautifying products are maximized.

Description

technical field [0001] The invention relates to the field of cosmetology, in particular to a primer, a probe and a kit for evaluating skin sun protection. Background technique [0002] SNP (single nucleotide polymorphism) refers to the variation of a single nucleotide on the genome, including transitions, transversions, deletions, and insertions. It is the most common genetic marker, and its carrying frequency in the population is greater than 1%. , and are widely used in the fields of human disease diagnosis, individualized drug use, and basic biological research. With the in-depth study of different SNP sites, through the combined detection of multiple SNP sites and subsequent data analysis, personalized health solutions can be formulated for the genetic information of different groups of people. [0003] The appearance of human skin is affected by internal and external factors. External factors include ultraviolet rays, environmental pollution, improper use of beauty pro...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6883C12Q2600/156
Inventor 纪志梁王毛阳刘晓瑜杨志云
Owner FUJIAN AIWO HEALTH BIOTECH CO LTD
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