Absorption and scattering coefficient reconstruction method in optical projection tomography

Abstract

The invention discloses an absorption and scattering coefficient reconstruction method in optical projection tomography (OPT). Transmitted light intensity is decomposed using the theory of series expansion; the scattering coefficient of biological tissues is reconstructed using a statistical iterative algorithm according to an OPT model and based on the characteristics of ballistic propagation signals and scattering signals in measured signals; the absorption coefficient of biological tissues is calculated based on the relationship of the attenuation coefficient, scattering coefficient and absorption coefficient; and finally, spatial distribution of the absorption coefficient of biological tissues and spatial distribution of the scattering coefficient of biological tissues are reconstructed at the same time. The method is applied to projection tomography reconstruction of weak scattering mediums. 3D reconstruction of the absorption coefficient and scattering coefficient of biological tissues is realized. The problem of scattering in OPT is solved effectively, the quality of OPT is improved, and information provided by the OPT technology is enriched.

Description

technical field [0001] The invention belongs to the technical field of medical image processing, in particular to an absorption and scattering coefficient reconstruction method in optical projection tomography. Background technique [0002] Optical projection tomography (OPT) is a new high-resolution three-dimensional molecular imaging technology, and its imaging principle is similar to that of X-ray computed tomography. OPT can obtain scanning sample structure images, and can use fluorescent dyes or fluorescent proteins for molecular specific labeling to realize molecular characteristic imaging, and the equipment is low in cost and easy to use. [0003] OPT imaging suffers from photon scattering since biological samples are not cleared. The conventional OPT imaging algorithm only considers the absorption characteristics of biological tissues to photons, and can only reconstruct the absorption coefficient of photons but not the scattering coefficient of photons. When using...

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Problems solved by technology

[0004] The purpose of the present invention is to provide a method for reconstruction of absorption and scattering coefficients applied in optical projection tomography of weakly scattering media, aiming at solving the problem that the conventional OPT imaging algorithm only considers the absorption characteristics of biological tissues to photons and cannot reconstruct photons at the same time Problems with Absorption Coefficient and Photon Scattering Coefficient

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