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Wettable bacterial powder capable of efficiently degrading cypermethrin and 3-phenoxybenzoic acid and its preparation method

A technology of phenoxybenzoic acid and cypermethrin, which is applied in the field of wettable bacterial powder and its preparation, can solve the problems of male sperm DNA fragmentation sperm concentration, difficulty in degrading 3-phenoxybenzoic acid, poor resistance to environmental adverse factors, etc. , to achieve the effect of shortening the wetting time, short wetting time and easy raw materials

Active Publication Date: 2019-02-19
黑龙江棒星食品科技股份有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, microbial degradation of pyrethroid pesticides often results in the accumulation of 3-phenoxybenzoic acid
The toxicity and mobility of 3-phenoxybenzoic acid in the environment are stronger than that of pyrethroid pesticides, which will interfere with the normal secretion of human sex hormones, cause DNA breakage in male sperm and reduce sperm concentration
However, these bacteria powders or bacteria liquids are difficult to degrade 3-phenoxybenzoic acid, which will cause new safety problems
In addition, bacteria liquid generally has problems such as short storage period and poor resistance to environmental adverse factors; bacteria powder also has problems such as poor wettability and inconvenient spraying.

Method used

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  • Wettable bacterial powder capable of efficiently degrading cypermethrin and 3-phenoxybenzoic acid and its preparation method
  • Wettable bacterial powder capable of efficiently degrading cypermethrin and 3-phenoxybenzoic acid and its preparation method
  • Wettable bacterial powder capable of efficiently degrading cypermethrin and 3-phenoxybenzoic acid and its preparation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] In this example, the raw materials used and the mass parts of each raw material are: 55kg composite bacterial liquid, 33kg diatomaceous earth, 4kg sodium lignosulfonate, 2kg Tween 80, 1.5kg skimmed milk powder and 1.5kg trehalose;

[0027] The composite bacteria solution consists of a bacterium concentration of 5.0×10 7 cfu / mL of Bacillus licheniformis B-1 bacteria liquid and bacteria concentration is 5.0×10 8 The cfu / mL Sphingomonas SC-1 bacteria solution is formulated at a volume ratio of 1:2.5 and mixed evenly to form;

[0028] The process steps are as follows:

[0029] (1) Preparation of bacterial agent mixture

[0030] Add diatomaceous earth into the compound bacterial liquid and stir evenly to obtain carrier-bacterial liquid mixture; add Tween 80, sodium lignosulfonate, skimmed milk powder and trehalose into sterile water equal to the volume of the mixed bacterial liquid and stir until they are completely dissolved and mixed uniformly to obtain the auxiliary ag...

Embodiment 2

[0042] In this example, the raw materials used and the mass parts of each raw material are: 47kg composite bacterial liquid, 38kg diatomaceous earth, 7kg sodium lignosulfonate, 4kg Tween 80, 2.0kg skimmed milk powder and 2.0kg trehalose;

[0043] The composite bacteria solution consists of a bacterium concentration of 5.0×10 8 cfu / mL of Bacillus licheniformis B-1 bacteria liquid and bacteria concentration is 5.0×10 9 The cfu / mL Sphingomonas SC-1 bacteria solution is formulated with a volume ratio of 1:2.3 and mixed evenly to form;

[0044] The process steps are as follows:

[0045] (1) Preparation of bacterial agent mixture

[0046] Add diatomaceous earth into the compound bacterial liquid and stir evenly to obtain carrier-bacterial liquid mixture; add Tween 80, sodium lignosulfonate, skimmed milk powder and trehalose into sterile water equal to the volume of the mixed bacterial liquid and stir until they are completely dissolved and mixed uniformly to obtain the auxiliary ...

Embodiment 3

[0053] In this example, the raw materials used and the mass parts of each raw material are: 35kg compound bacterial liquid, 42kg diatomaceous earth, 10kg sodium lignosulfonate, 7kg Tween 80, 3.0kg skimmed milk powder and 3.0kg trehalose;

[0054] The composite bacteria solution consists of a bacterium concentration of 5.0×10 7 cfu / mL of Bacillus licheniformis B-1 bacteria liquid and bacteria concentration is 5.0×10 9 The cfu / mL Sphingomonas SC-1 bacteria solution is formulated with a volume ratio of 1:2 and mixed evenly;

[0055] The process steps are as follows:

[0056] (1) Preparation of bacterial agent mixture

[0057] Add diatomaceous earth into the compound bacterial liquid and stir evenly to obtain carrier-bacterial liquid mixture; add Tween 80, sodium lignosulfonate, skimmed milk powder and trehalose into sterile water equal to the volume of the mixed bacterial liquid and stir until they are completely dissolved and mixed uniformly to obtain the auxiliary agent solu...

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Abstract

The invention discloses wettability fungus powder capable of effectively degrading cypermethrin and 3-phenoxy benzoic acid and a preparation method. The wettability fungus powder comprises the raw materials of composite fungus liquid, diatomaceous earth, sodium lignin sulfonate, tween 80, skim milk powder and trehalose; and the composite fungus liquid consists of bacillus licheniformis B-1 fungus liquid and sphingomonas SC-1 fungus liquid. The method comprises the following steps: (1) the diatomaceous earth is added in the composite fungus liquid to obtain a carrier-fungus liquid mixture; the tween 80, the sodium lignin sulfonate, the skim milk powder and the trehalose are added in sterile water having the same volume with the composite fungus liquid to obtain auxiliary solution; and the auxiliary solution and the carrier-fungus liquid mixture are uniformly mixed to obtain fungicide mixed liquid; and (2) the fungicide mixed liquid obtained in the step (1) is dried by the following process parameters in sequence: a temperature from -46 to -48 DEG C is kept by 1.0-3.0 h; a temperature from -36 to -40 DEG C is kept by 1.0-3.0 h; a temperature from -26 to -30 DEG C is kept by 1.0-3.0 h; a temperature from -15 to -20 DEG C is kept by 1.0-3.0 h; a temperature from 0 to 4 DEG C is kept by 10-14 h; a temperature from 8 to 12 DEG C is kept by 1.0-3.0 h; a temperature from 30 to 40 DEG C is kept by 2.0-6.0 h; a temperature from 15 to 25 DEG C is kept by 20-24 h; and the wettability fungus powder is obtained.

Description

technical field [0001] The invention belongs to the field of pesticide cypermethrin degradation, in particular to a wettable bacterial powder capable of degrading cypermethrin and 3-phenoxybenzoic acid and a preparation method thereof. Background technique [0002] In recent years, with the partial or complete ban of organochlorine and organophosphorus insecticides, the use of pyrethroid pesticides has accounted for 30% of the world's insecticide market. Cypermethrin is one of the most widely used pyrethroid pesticides. However, with the continuous increase of its usage and scope, there are reports of its residue exceeding the standard in tea, fruits and vegetables and soil. Residual cypermethrin can enter the human body through the food chain and cause toxicity to the nervous, immune and reproductive systems. Because microbial degradation of cypermethrin is environmentally friendly, safe and efficient, it is widely used to eliminate residual cypermethrin in tea, fruits an...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A62D3/02B09C1/10A62D101/04A62D101/28
CPCA62D3/02A62D2101/04A62D2101/28B09C1/10B09C2101/00
Inventor 姚开赵甲元吴双贾冬英迟原龙李欣蔚徐颖超
Owner 黑龙江棒星食品科技股份有限公司
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