Method for extracting nuciferine in folium nelumbinis by aid of ultrasonic processes

A technology of lotus leaf base and ultrasonic method, applied in the field of laboratory testing, can solve the problems of complex extraction and testing process and long time, and achieve the effect of consistent accuracy, less sample consumption and good repeatability

Inactive Publication Date: 2016-08-31
WUZHOU INST FOR FOOD & DRUG CONTROL
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Problems solved by technology

[0004] The pharmacopoeia method takes several hours to test, and...

Method used

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  • Method for extracting nuciferine in folium nelumbinis by aid of ultrasonic processes
  • Method for extracting nuciferine in folium nelumbinis by aid of ultrasonic processes

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Embodiment 1

[0017] A method for extracting nuciferine in lotus leaves by ultrasonication, comprising the following steps:

[0018] Step 1: After crushing lotus leaves, sieve;

[0019] Step 2: Grinding after mixing lotus leaf powder and nano silicon dioxide, the weight ratio of lotus leaf powder and nano silicon dioxide is 19:1;

[0020] Step 3: Mix the mixture obtained in step 2 with an extraction solvent, the extraction solvent is a mixture of 1 part by weight of acetic acid, 4 parts by weight of methanol and 5 parts by weight of ethanol, and the weight ratio of the mixture to the extraction solvent is 1:9;

[0021] Step 4: After heating the mixture in step 3 to: 52°C, put the container containing the mixture into an ultrasonic cleaner for extraction to obtain an extraction mixture; the extraction time is 33 minutes; the ultrasonic frequency is 250KHz.

[0022] Step 5: After filtering the extraction mixture obtained in step 4, take 1ml and dilute it to 50ml with the extractant of the co...

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Abstract

The invention discloses a method for extracting nuciferine in folium nelumbinis by the aid of ultrasonic processes. The method includes steps of 1, smashing and then sieving the folium nelumbinis to obtain folium nelumbinis powder; 2, mixing the folium nelumbinis powder and nanometer silicon dioxide with each other and then grinding the folium nelumbinis powder and the nanometer silicon dioxide to obtain mixtures; 3, mixing the mixtures obtained at the step 2 and extraction solvents with one another; 4, arranging a container with mixtures in an ultrasonic cleaner and extracting the mixtures to obtain extract mixtures; 5, filtering the extract mixtures, then diluting the extract mixtures by the aid of extraction agents, analyzing diluted solution by the aid of liquid chromatography and measuring the content of the nuciferine. The method for extracting the nuciferine in the folium nelumbinis by the aid of the ultrasonic processes has the advantages that the nuciferine is extracted by the aid of the ultrasonic extraction processes and is detected by the aid of the liquid chromatography, and the method is high in detection precision and low in extraction and analysis operation difficulty.

Description

technical field [0001] The invention relates to the field of laboratory testing, in particular to a method for extracting nuciferine from lotus leaves by an ultrasonic method. Background technique [0002] The 2015 Pharmacopoeia recorded the extraction method of nuciferine in lotus leaves: take about 0.5g of the coarse powder of this product, accurately weigh it, put it in a stoppered conical flask, add 50ml of methanol accurately, weigh it, heat and reflux for 2.5 hours , let it cool, weigh again, make up for the lost weight with methanol, shake well, filter, accurately measure 5ml of the filtrate, put it in a 10ml measuring bottle, add water to the mark, shake well, and you get it. [0003] The detection conditions of liquid chromatography are as follows: octadecylsilane bonded silica gel is used as filler; acetonitrile-triethylamine glacial acetic acid (27:70.6:1.6:0.78) is used as mobile phase; detection wavelength is 270nm. The number of theoretical plates should not b...

Claims

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Application Information

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IPC IPC(8): G01N30/02B01D11/02
Inventor 陈学松王丽丽韦涛梁美艳黄新惠
Owner WUZHOU INST FOR FOOD & DRUG CONTROL
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