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A salt-tolerant rhizosphere growth-promoting Enterobacter cloacae and its application

A technology of Enterobacter cloacae and bacterial agent, applied in the field of salt-tolerant rhizosphere growth-promoting Enterobacter cloacae, can solve the problems of fragile ecological environment, harsh soil conditions, difficult development and utilization, etc. The amount of water, the effect of promoting the growth of white wax

Active Publication Date: 2019-05-28
SHANDONG FOREST SCI RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The soil conditions in these saline-alkaline areas are harsh, the land productivity is low, it is difficult to develop and utilize, and the ecological environment is extremely fragile

Method used

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  • A salt-tolerant rhizosphere growth-promoting Enterobacter cloacae and its application
  • A salt-tolerant rhizosphere growth-promoting Enterobacter cloacae and its application
  • A salt-tolerant rhizosphere growth-promoting Enterobacter cloacae and its application

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Experimental program
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Effect test

Embodiment 1

[0031] Example 1 Screening of Enterobacter cloacae TIA062 of the present invention

[0032] (1) Screening process

[0033] Two samples of Baixa rhizosphere soil samples were collected from the Yellow River Delta. Bacterial isolates were isolated from rhizosphere soil samples by serial dilution method. Purify the isolated bacteria by the three-section line method, and judge whether the strain is purified by microscopic examination, number the purified bacteria, pick a single colony, and transfer it to an LB slant for future use.

[0034] Inoculate the bacteria into the R2A liquid medium containing L-tryptophan, culture at 28°C, 180rpm, on a shaker for 4 days. Take 50 microliters of bacterial suspension and drop it on a white ceramic plate, and add 50 microliters of Salkowski colorimetric solution (50ml 35% HCLO 4 +1ml 0.5mol / L FeCl 3 ). The colorimetric solution added with 50 mg / L IAA was used as a positive control. The white ceramic plate was placed at room temperature i...

Embodiment 2

[0045] Example 2 Growth-promoting effect on white wax under salt stress

[0046] Activate the TIA062 stored on the slant in LB (peptone 10g; NaCl 10g; yeast extract 5g; agar 15-20g; water 1000ml; pH7.0-7.2, aliquot and sterilize 20min) on the plate. Pick a single colony into the corresponding 10ml liquid medium, and culture at 37°C and 180rpm for 24h. The seed solution was inoculated into the corresponding 50ml liquid culture medium according to the inoculum amount of 1% for fermentation, and cultured at 37° C. and 180 rpm for 2 days.

[0047] The plant material is selected from the ash container seedlings cultivated in that year. The substrate materials used for seedling cultivation are peat and vermiculite. The peat comes from Shancheng Town, Meihekou City, Jilin Province, and vermiculite is a commonly used material. According to the volume ratio of peat: vermiculite = 3:1 mixed as a seedling substrate.

[0048] Add NaCl according to the weight calculation, adjust the so...

Embodiment 3

[0052] A microbial mixture is characterized in that it contains the bacterial strain Enterobacter cloacae (Enterobactercloacae) TIA062 of the present invention, and also contains other microorganisms that have salt-alkali tolerance and can coexist with the bacterial strain of the present invention. It has been verified by experiments that the microbial mixture can significantly promote the high growth of white wax, increase the weight of shoots and roots, and can reduce the physiological damage caused by salt stress to white wax.

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Abstract

The invention discloses a strain of salt-tolerant rhizosphere growth-promoting Enterobacter cloacae and its application. The strain is named Enterobacter cloacae (Enterobacter cloacae) TIA062, and has been preserved in China's Microbiological Culture Collection Management on January 7, 2016. Committee General Microbiology Center, deposit number is CGMCC No.11974. The salt-tolerant rhizosphere growth-promoting enterobacter cloacae of the invention can improve the salt-alkali tolerance of white wax. The salt-tolerant rhizosphere growth-promoting Enterobacter cloacae of the present invention is inoculated around the root system of white wax, and after a certain period of salt stress, it can increase the water content of the root system of white wax and promote the growth of white wax compared with no inoculation agent. Therefore, the present invention The invention can be used as an inoculant in a saline environment, and provides excellent strain resources for improving the saline-alkali resistance of white wax.

Description

technical field [0001] The invention relates to a salt-tolerant rhizosphere growth-promoting Enterobacter cloacae and application thereof. Background technique [0002] Soil salinization is a worldwide resource and ecological problem. The total amount of various types of saline soil in my country is 1.487 billion mu, of which, modern salinized soil is about 554 million mu; residual salinized soil is about 673 million mu ; Potential salinized soil is about 260 million mu. The soil conditions in these saline-alkaline areas are harsh, the land productivity is low, it is difficult to develop and utilize, and the ecological environment is extremely fragile. [0003] At present, my country's improvement measures for saline-alkali land mainly focus on the four major systems of physics, water conservancy, biology and chemistry. Among them, biological improvement mainly applies the synergistic effect of highly efficient salt-tolerant microorganisms and plants, which can protect the ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12N11/00A01N63/00A01P21/00C12R1/01
CPCA01N63/00C12N1/20C12N11/00C12N1/205C12R2001/01
Inventor 杨庆山刘波刘方春丁延芹李淑杰杜秉海井大炜马海林
Owner SHANDONG FOREST SCI RES INST
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