Method for establishing Mongolian gerbil orthotopic liver transplantation model and method for separating hepatic stellate cells

A technology of hepatic stellate cells and long-clawed gerbils, applied in cell dissociation methods, animal cells, vertebrate cells, etc., to achieve the effect of fierce animal resistance, ideal effect, and reduction of biliary obstruction
CN105941329AInactive Publication Date: 2016-09-21ZHEJIANG ACAD OF MEDICAL SCI
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
ZHEJIANG ACAD OF MEDICAL SCI
Publication Date
2016-09-21
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses a method for establishing a Mongolian gerbil orthotopic liver transplantation model and a method for separating hepatic stellate cells. The method for establishing the Mongolian gerbil orthotopic liver transplantation model comprises the steps that a donor is narcotized, and a grafted liver is cut and taken out of the donor; a receptor is narcotized, the liver is cut out, the grafted liver is implanted into the receptor with the liver cut out; the survival rate of the Mongolian gerbil orthotopic liver transplantation model is calculated. Mongolian gerbil orthotopic liver transplantation is executed, changes of the Mongolian gerbil orthotopic liver transplantation model are observed, and the survival rate is calculated. The method for separating the hepatic stellate cells of Mongolian gerbil comprises the steps that chloral hydrate is used for intraperitoneal injection to narcotize the male Mongolian gerbil, or the male Mongolian gerbil which dead just now is selected, and the grafted liver is cut and taken out of the donor; the hepatic stellate cells are separated out of the grafted liver and obtained. According to the method for separating the hepatic stellate cells, the yield and survival rate of the hepatic stellate cells can be increased, and the purity of HSCs can be guaranteed.
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Description

technical field

[0001] The invention relates to the technical field of gerbil liver transplantation and cell separation, in particular to a method for establishing a model of orthotopic liver transplantation in a gerbil and a method for isolating hepatic stellate cells. Background technique

[0002] A stable and reliable animal model of liver transplantation is the basis of clinical liver transplantation and immune tolerance research. Only when a mature and stable animal model is used as a technical platform, can the development of organ transplantation and immune tolerance research achieve a qualitative leap. Although the rat liver transplantation model is currently the most commonly used animal model, the stability and reproducibility of the closed group rat model needs to be further improved, while the stability of the inbred rat model has been improved, but due to lack of resources Relatively scarce, difficult to obtain, and relatively expensive, so it is very important...

Claims

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