Qualitative detection method for illegal ingredient Sudan red in raw materials of feeds

Abstract

The invention discloses a qualitative detection method for an illegal ingredient, i.e., Sudan red, in raw materials of feeds. According to the method, suspected areas are primarily screened through microscopic image processing, so a detection scope is reduced; then a microscopic Raman imaging spectrometer is used for detecting the Raman spectra of the suspected areas, the spectra are pretreated so as to obtain training samples, an IVISSA method sensitive to classes is used for waveband selection, and the IVISSA method can select characteristic peaks of Sudan red substantially different from characteristic peaks of other components, which enables detection efficiency and accuracy to be improved; the average spectrum of each training sample is taken and incorporated into a standard spectrum matching database; and the microscopic Raman spectrum of an unknown sample is acquired according to coordinates of detection points obtained in primary image election, then same spectral pretreatment is carried out, and a same wavelength variable is chose for one-to-one matching with each spectrum in the standard spectrum matching database according to a feature enhanced spectral angle matching method so as to determine whether the unknown sample contains Sudan red or not. The qualitative detection method has the advantages of rapid detection, good stability, and lower false positive rate and detection limits.

Description

Technical field [0001] The invention relates to the technical field of feed safety analysis in agriculture, in particular to a qualitative detection method of Sudan red, an illegal additive in feed materials. Background technique [0002] At present, as one of the largest producers of livestock and poultry breeding in the world, my country's livestock and poultry food safety situation is relatively severe, and food safety incidents are emerging one after another. Food safety is closely related to the health of all human beings. As animal feed is the source and key link of animal food safety, its nutritional value and safety have also attracted more and more attention from all walks of life. In recent years, some illegal companies have incorporated the industrial dye Sudan Red into poultry feed materials to make the products have a brighter appearance, easy to sell, reduce costs, and seek profits. Sudan red contains azo in its chemical structure, and the nature of this structure ...

AI Technical Summary

Problems solved by technology

[0006] The technical problem of the present invention is to overcome the deficiencies of the prior art, and provide a simple, fast and efficient qualitative detection method for the illegal additive Sudan Red in the feed raw materials, so as to solve the problem of complex operation, poor repeatability and difficulty in realizing trace amounts in the prior art. The problem of small detection and imaging area and high correlation of spectral data

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