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Aspergillus oryzae pcr detection primer and its application

A technology for detecting primers and Aspergillus oryzae, which is applied in the field of Aspergillus oryzae PCR detection primers, can solve the problems of low specificity of detection primers and single detection target, and achieves the effects of good specificity, single detection target and strong stability.

Inactive Publication Date: 2019-05-21
HUAZHONG AGRI UNIV
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Problems solved by technology

[0005] The purpose of the present invention is to overcome the defects of traditional oryza koji PCR specificity detection target is single, detection primer specificity is not strong, a kind of rice koji fungus specific nested PCR detection primer with strong specificity and high sensitivity is provided, the present invention Uses of the primer are also provided

Method used

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  • Aspergillus oryzae pcr detection primer and its application
  • Aspergillus oryzae pcr detection primer and its application
  • Aspergillus oryzae pcr detection primer and its application

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Embodiment 1

[0024] 1. Prediction of the whole genome of Aspergillus oryzae, screening of specific detection targets and design of primers

[0025] 1.1 Whole genome prediction of Aspergillus oryzae

[0026] According to the sequenced HWD-2 genome of Aspergillus oryzae obtained by sequencing (Jia et al., 2015), the Aspergillus oryzae gene was sequenced using the AUGUSTUS (Version 2.5.5) software and the Fusarium graminearum genome as a reference model. predict. The final prediction analysis of Aspergillus oryzae contains 8231 genes, and the corresponding DNA sequence and protein sequence were obtained.

[0027] 1.2 Construction of local database used for comparison and screening of Aspergillus oryzae-specific target genes

[0028] In order to reduce the computational load of the computer, reduce the configuration requirements of the computer and save time, two databases are used, one is the database containing the predicted protein sequences of 43 species (Table 1), and the other is the N...

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Abstract

The invention discloses a group of ustilaginoidea virens PCR detection primers. Specific detection targets of ustilaginoidea virens are found through a method for comparing genomes, and the detection primers are designed. Compared with traditional ustilaginoidea virens PCR detection primers, the detection primers have the advantages of being good in specificity, high in stability and high in sensitivity, and the false positive phenomenon is well controlled in the detection process; meanwhile, ustilaginoidea virens can be accurately detected, and good tools are provided for field detection of ustilaginoidea virens, circulation research of ustilaginoidea virens diseases, prediction of the ustilaginoidea virens diseases and the like.

Description

technical field [0001] The invention belongs to the field of biology, and in particular relates to PCR detection primers for Aspergillus oryzae and uses thereof. Background technique [0002] Rice false smut (Rice false smut) is an important rice fungal disease caused by Ustilaginoidea virens, which occurs in all rice regions around the world. In recent years, with the change of rice cultivation mode, the increase of nitrogen fertilizer application in rice fields, and the large-scale promotion of new high-yield dense-ear hybrid rice, rice false smut has gradually become the main disease of rice. The large-scale occurrence of rice false smut not only affects the yield and quality of rice, but also the aspergillus produced in rice koji has toxic effects on humans and animals, seriously affecting food security. [0003] Current studies suggest that Aspergillus oryzae overwinters in soil or diseased residues with chlamydospores and sclerotia, germinates to form conidia or ascos...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6895C12Q1/04C12N15/11C12R1/645
CPCC12Q1/6895
Inventor 郑露汤近天黄俊斌张耀
Owner HUAZHONG AGRI UNIV
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