Pyropia haitanensis pure line seedling cultivation method

A method for cultivating and a technology for seaweed, applied in the field of seaweed, can solve problems such as inability to realize pure-bred production, uneven seedlings, and complex genetic background of provenance, and achieve the advantages of promoting pure-bred production, good quality, and guaranteeing The effect of cultivation yield

Active Publication Date: 2016-10-12
JIMEI UNIV
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Problems solved by technology

The seedlings cultivated by this method, due to inconsistent sources of thallus, are a large heterogeneous line, and the genetic background of the provenance is complex, resulting in mixed seedlings, which cannot achieve pure breeding and ensure the quality of seedlings (Huang Chunkai, Yan Zhihong .Indoor Cultivation Technology of Porphyra Seedlings[J].Aquatic Science and Technology Information,2004,31(5):200-202.)

Method used

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Embodiment Construction

[0024] Below in conjunction with specific embodiment, further illustrate the present invention.

[0025] 1. Select a plant of algae "Minfeng No. 1" fronds in the natural sea area, clean the surface of the fronds with a brush, and then dry them in the shade and store them in a freezer (-18°C); take out the frozen algae and use them again After rinsing with disinfected seawater, recover in still water and weak light for 24 hours (cultivation conditions: temperature 20±1°C, light 1000Lx, 12L:12D).

[0026] 2. Use sterilized scissors to cut 0.5-1cm 2 The vegetative tissue blocks of algae were soaked in 2mol / L glucose solution for 10 minutes, then cut into small pieces with scissors, and placed in conch enzyme solution for enzymolysis (enzyme solution formula: 0.12g conch enzyme + 1.27 g mannitol + 2.2mg anhydrous CaCl 2 +2.4mgMgSO 4 +10ml of sterilized seawater with a salinity of 37, pH 6.0~6.2), enzymolysis conditions: dark shaking, temperature 27°C, enzymolysis time 1~2h; Wh...

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Abstract

The invention relates to a pyropia haitanensis pure line seedling cultivation method, and relates to pyropia haitanensis. According to the invention, the surface of the a single pyropia haitanensis thallus is cleaned, and the pyropia haitanensis thallus is dried in shade and is cryopreserved; the cryopreserved pyropia haitanensis thallus is subjected to resuscitation culture in still water under low light; a nutritive tissue block is soaked in a glucose solution; the soaked nutritive tissue block is cut into small blocks, and the small blocks are subjected to enzymolysis in a conchase solution; cell dissociation condition is observed; when free unicellular or multicellular masses from algae fragments are observed, cell sap is filtered; a filtrate is centrifuged, and a supernatant is discarded and a precipitate is retained; centrifugation is repeated after washing; collected cells are subjected to dark culture in sterilized seawater; cultivation is continued in a 1 / 2 MES culture solution; when the culture solution restores normal seawater salinity, culturing is continued for 20-30 days; conchocelis is obtained by suction, such that pure line germplasm conchocelis is obtained; still water culture is carried out for conserving the breed; primary amplification, secondary amplification and third-level amplification are carried out, such that pure line free conchocelis are obtained; and the pure line free conchocelis is transplanted into seashells for carrying out seedling cultivation.

Description

technical field [0001] The invention relates to algae laver, in particular to a method for cultivating altar laver pure line seedlings. Background technique [0002] Pyropia haitanensis is one of the two main types of laver artificially cultivated in my country. It is native to the coast of Fujian Province and is a unique warm temperate species in my country. Since the success of artificial cultivation in the 1960s, the cultivation area of ​​Porphyra laver has been continuously expanded, and it has become one of the main targets of mariculture in southern my country, and its output accounts for more than 75% of the total production of laver in the country. [0003] The life history of Porphyra can be divided into two generations with completely different morphological structures: the thallus generation and the filament generation. The fronds are the objects of artificial cultivation and harvesting at sea, while the filaments are the objects of indoor artificial seedlings, w...

Claims

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Application Information

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IPC IPC(8): A01G33/02
Inventor 谢潮添徐燕纪德华陈昌生
Owner JIMEI UNIV
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