Application of leonurine to preparation of medicine for treating vascular dementia

A technology of vascular dementia and motherwort, which is applied in drug combination, nervous system diseases, etc.

Inactive Publication Date: 2016-10-12
NANKAI UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to find and solve the problem of preparing and preventin

Method used

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  • Application of leonurine to preparation of medicine for treating vascular dementia
  • Application of leonurine to preparation of medicine for treating vascular dementia
  • Application of leonurine to preparation of medicine for treating vascular dementia

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Example 1. Leonurine improves the spatial learning and memory ability of rats

[0036]After the experimental rats were successfully anesthetized with 10% chloral hydrate, they were fixed on the operating table in a supine position, and after routine disinfection, an incision was made in the middle of the rat’s neck, and the muscles were bluntly separated to expose the bilateral common carotid arteries and threaded with a No. 0 silk thread. After ligation, the muscles were reset and the skin was sutured. After the rats woke up, they were put back into the cage for routine feeding. The operation of the sham operation group was the same as that of the model, but only the bilateral common carotid arteries were separated without ligation. Rats in the postoperative model + motherwort group were given 60 mg / kg / day of motherurine by intragastric administration for 3 weeks. After the administration, the Morris water maze test (MWM) was carried out to test the spatial learning a...

Embodiment 2

[0037] Example 2. Effect of Leonurine on Percentage of Residence Time in Each Quadrant in the Reverse Learning Training Process of Rats

[0038] The specific implementation method is the same as in Example 1. Record the percentage of swimming time in each quadrant of the rats in the reverse learning phase (day 6 to day 7) (see Figure 7 ,8,9,10), and recorded the data of rat swimming trajectory (see Figure 11 ).

[0039] The result is as figure 2 As shown, compared with the sham operation group, the time of swimming exploration in the original target quadrant (zone 1) of the rats in the model group was significantly prolonged on the 7th day (see Figure 7 ) (P Figure 7 ), increasing its swimming time in the new target quadrant (zone 4) (see Figure 10 ) (P<0.05).

Embodiment 3

[0040] Example 3. Effect of Leonurine on LTD in Rat Hippocampus CA3-CA1 Region

[0041] Rats were anesthetized with 30% urethane intraperitoneally (4ml / kg), fixed on a stereotaxic instrument, exposed the skull, and positioned CA3 (4.2 mm behind the bregma, 3.5 mm beside the midline, and 2.5 mm below the dura mater) and CA1 area (3.5mm posterior to bregma, 2.5mm lateral to midline, 2.0mm subdural). After obtaining a stable representative waveform, the baseline was recorded for 20 min, and then a low-frequency stimulation of 900 pulses at 1 Hz was given for a total of 15 min to induce long-term post-synaptic depression. Then restore the single-stimulus method during base recording, and record for 60 minutes. The result is as image 3 As shown, compared with the sham operation group, the slope of the fEPSPs of the model group rats was significantly improved (see Figure 12 and Figure 13 ) (P Figure 12 and Figure 13 ) (P<0.05).

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Abstract

The invention belongs to the field of pharmacy, relates to application of leonurine to preparation of a medicine for treating dementia and in particular relates to application of the leonurine to preparation of a medicine for treating vascular dementia. A rat VD model is successfully copied and an experiment result shows that the leonurine can remarkably improve cognitive impairment caused by the VD and alleviate impairment of long-term postsynaptic inhibition; the content of hydrogen peroxide (H2O2) and glutamate is reduced, the specific value of NR2A/2B is improved and the expression of LC3II/LC3I and Beclin-1 is regulated down, so that autophagic flux is inhibited. The leonurine can be used as a treatment medicine for treating the dementia, especially brain injury diseases caused by the VD.

Description

technical field [0001] The invention belongs to the field of pharmacy, and relates to the use of leonurine in the preparation of medicines for preventing and treating target organs, especially the use in the preparation of medicines for preventing and treating vascular dementia caused by chronic hypoperfusion. Background technique [0002] Chronic cerebral hypoperfusion is an important factor causing vascular dementia (VD). It refers to a pathological state of nervous system damage caused by long-term brain tissue perfusion lower than the physiological threshold due to various reasons. The main manifestation is cognitive impairment. Vascular dementia not only seriously damages the health of patients and reduces their quality of life, but also brings a heavy burden to society and families. But at present, the pathogenesis of vascular dementia caused by chronic cerebral hypoperfusion is not very clear, and there is no special and effective treatment method, and symptomatic t...

Claims

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Application Information

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IPC IPC(8): A61K31/235A61P25/28
CPCA61K31/235
Inventor 刘春华杨卓朱依谆张涛古险峰高静
Owner NANKAI UNIV
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