Method for separating anti-RSV-virus effective constituents after compatibility of pulsatilla chinensis and holotrichia diomphalia extracts

An effective part, antiviral technology, applied in medical preparations containing active ingredients, antiviral agents, pharmaceutical formulations, etc., can solve the problem of potential toxicity of patients and medical staff, save manpower and material resources, improve treatment effect, purposeful effect

Inactive Publication Date: 2016-10-26
周长征
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Problems solved by technology

However, studies have found that ribavirin-resistant strains appeared in the cotton rat model infected with respiratory syncytial virus, and it is potentially toxic to patients and medical staff, so this drug is only used in patients with severe RSV infection, so Exploring new substances with inhibitory effect on respiratory syncytial virus is necessary for the times

Method used

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Examples

Experimental program
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Effect test

example 1

[0019] Example 1 (1) Weigh a certain amount of Pulsatilla pulsatillae and grub, add 10 times the amount, heat and reflux to extract twice, the first time for 2 hours, and the second time for 1.5 hours, combine the two extracts, and concentrate under reduced pressure to the concentration per milliliter of liquid medicine Containing 1 gram of medicinal materials, add ethanol to adjust the concentration of ethanol in the solution to 70%, and let it stand for 24 hours.

[0020] (2) Filter the alcohol precipitation solution obtained in (1) under reduced pressure to obtain a precipitate, and dissolve the precipitate with distilled water sonication.

[0021] (3) Apply the solution obtained in (2) to the treated X-5, D101 macroporous adsorption resin, rinse with water, take one bottle per 200ml, and rinse until colorless.

[0022] (4) Merge the parts eluted with middle water in (3), concentrate to a certain volume, and carry out antiviral research separately.

[0023] (5) The water e...

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Abstract

The invention belongs to traditional Chinese medicine active ingredient extraction process and antiviral effective part separation, and mainly relates to a method for separating anti-RSV-virus effective constituents after compatibility of pulsatilla chinensis and holotrichia diomphalia extracts. The method particularly comprises the following steps: conducting reflux extraction on pulsatilla chinensis and holotrichia diomphalia by adopting distilled water, conducting alcohol precipitation, resisting viruses, conducting dissolution and precipitation, separating the pulsatilla chinensis extract by adopting X-5 type macroporous adsorption resin to achieve a better effect, separating the holotrichia diomphalia extract by adopting D101 type macroporous adsorption resin to achieve a better effect, and determining the anti-virus effective parts. Then the effective parts of the two medicines are in compatibility according to the proportion of 9:1, 8:2, 7:3, 6:4, 5:5, 4:6, 3:7, 2:8 and 1:9 respectively, so as to conduct anti-RSV-virus research again. Through adoption of the anti-virus tracing method, the fact that after the compatibility of the anti-virus effective parts of the pulsatilla chinensis and the holotrichia diomphalia, the anti-virus effect is better, and the optimal compatibility proportion is obtained.

Description

technical field [0001] The present invention relates to the production and extraction process of active ingredients of traditional Chinese medicine, new ideas and new methods for extracting antiviral effective parts of Pulsatilla pulsatillae and grubs and new applications in the field of pharmacy, and specifically relates to extraction, separation and purification of Pulsatilla pulsatillae and grubs against respiratory syncytia by means of antiviral tracking The active ingredients of the virus, and the antiviral research after the active ingredients are compatible. Background technique [0002] In recent years, due to the sudden invasion of various viruses, such as SARS virus, H1N1, etc., each virus outbreak will cause panic or even death in humans, so it is very important to find suitable antiviral drugs. [0003] Pulsatilla is the dry root of Pulsatilla chinensis, also known as old man's flower, baldhead grass, powder grass, and old crown flower. It is born in mountains, b...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/71A61P31/14A61K35/64
CPCA61K36/71A61K35/63A61K2236/331A61K2236/53A61K2236/55A61K2300/00
Inventor 周长征牛文斐侯宝山张加泽王清
Owner 周长征
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