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A kind of extraction method, detection method and application of two-color jackfruit extract

A detection method, jackfruit technology, applied in the extraction field of two-color jackfruit extract, can solve the problems of failing to visually express the comprehensive extraction efficiency of chemical composition groups, characterizing the composition characteristics of samples, and failing to reflect drug activity, etc., to achieve good results. Medicinal prospects, strong pharmacological effects, and extensive medicinal value

Inactive Publication Date: 2019-12-13
YULIN NORMAL UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] The extraction process of traditional Chinese medicine is mainly based on the total amount of medicinal material extract, the content of a single chemical component, the extraction rate of major components, or the amount of index components in the extract in the prescription to evaluate the pros and cons of the process, but these indicators have not The compositional characteristics of the sample are more comprehensively characterized, but the comprehensive extraction efficiency of the chemical composition group in the sample and the corresponding chemical composition cannot be intuitively expressed
In the prior art, the extraction of traditional Chinese medicine is mostly a single chemical component or the content of multiple chemical components as the evaluation index. However, since most of the chemical components in traditional Chinese medicine are not clear, such detection methods can only accurately mark the content of a certain component, but cannot reflect the content of the drug. activity, difficult to correlate with efficacy

Method used

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  • A kind of extraction method, detection method and application of two-color jackfruit extract
  • A kind of extraction method, detection method and application of two-color jackfruit extract
  • A kind of extraction method, detection method and application of two-color jackfruit extract

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Effect test

Embodiment 1

[0027] The preparation of embodiment 1, two-color jackfruit extract

[0028] According to the single factor test, the extraction method, extraction solvent, solvent concentration, solid-liquid ratio, extraction time, extraction times, extraction temperature, etc. have an important influence on the extraction rate of the maple lotus. The group selects the best extraction parameters for the evaluation index. Three parallel volumes were extracted from each replicate and injected separately. After integrating the chromatograms of the three parallel quantities, the average values ​​of the peak areas corresponding to the 13 main peaks were obtained. The optimal extraction method was selected by comparing the average values ​​of the corresponding peak areas of the 13 main peaks in the chromatograms obtained by different extraction methods.

[0029] Chromatographic column: ZORBAX SB-C18 (5μm 4.6ⅹ150mm); mobile phase: acetonitrile-0.2% acetic acid, degassed by ultrasonic wave before ...

Embodiment 2

[0067] Embodiment 2, two-color jackfruit extract antidepressant activity

[0068] Preparation of test sample dichromatic jackfruit extract for experiment

[0069] Extract preparation

[0070] Take an appropriate amount of dried medicinal powder of jackfruit two-color, heat reflux with 80% ethanol, the ratio of solid to liquid is 1:60, the extraction time is 1 hour, the extraction temperature is 90°C, the number of extractions is 2 times, filter to obtain the extract of jackfruit two-color, and concentrate under reduced pressure to Dry to obtain the extract of jackfruit two-color, take part of the extract of jackfruit two-color and dissolve it in water to form a suspension, and extract it with petroleum ether, ethyl acetate, and n-butanol in turn to obtain petroleum ether, ethyl acetate, and n-butanol extract and water extracts were concentrated under reduced pressure to obtain petroleum ether, ethyl acetate, n-butanol and water extracts, and set aside.

[0071] 2. Effects of...

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Abstract

The invention discloses an extracting method of artocarpus styracifolius pierre extract. The extracting method comprises the steps of taking dry artocarpus styracifoliius pierre, conducting pulverization, then conducting extraction for 1-3 times with ethanol, and then conducting concentration and drying, so that the artocarpus styracifoliius pierre extract containing active sites for promoting blood circulation to remove blood stasis is obtained. The ratio of heterophyllous wing seedtree roots to ethanol is 1g: (30-90) ml, and extraction via ethanol is conducted for 0.5-2 hours each time. The extracting method is high in extraction accuracy and activity of the extract. The invention further provides a detection method of the artocarpus styracifoliius pierre extract. The extracting method is accurate in extraction and efficiency. The detection method is high in detection precision. Meanwhile, the invention further discloses application of the artocarpus styracifoliius pierre extract to preparation of anti-depression medicine.

Description

technical field [0001] The invention relates to the technical field of drug analysis, in particular to an extraction method, a detection method and an application of a dichromatic jackfruit extract. Background technique [0002] Two-color jackfruit is a plant of the genus Jackfruit in the family Moraceae, also known as Hongfenghe, Hongshanmei, Banfenghe, etc. It is rich in Guangdong, Hainan, Guangxi, Yunnan and other places. effect. For the treatment of rheumatoid arthritis, lumbar muscle strain, chronic low back pain, hemiplegia and other diseases. Ren Gang and others have successively studied the chemical composition of the root bark of Jackfruit dichromatica, and the gas chromatography-mass spectrometry analysis of the chemical components of the volatile oil of Jackfruit dichromatica leaves, in order to provide a basis for further understanding and utilization of this plant resource. Lu Haixiao and others studied the chemical constituents of the stem of Jackfruit dichro...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/60A61P25/24G01N30/88G01N30/02G01N30/06G01N30/14
CPCA61K36/60A61K2236/333G01N30/02G01N30/06G01N30/14G01N30/88G01N2030/027G01N2030/062G01N2030/8809
Inventor 卢海啸
Owner YULIN NORMAL UNIVERSITY
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