A kind of black bone rattan compound extract and its preparation method and application

A kind of extract, the technology of the black bone rattan, applied in the field of the compound extract of the black bone rattan and its preparation and application, can solve the problems of inconvenient use for patients, low content of active ingredients, saving, etc.

Inactive Publication Date: 2016-03-09
SICHUAN NORMAL UNIV
View PDF4 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] In the above-mentioned patents, the medicinal effect of the compound is better, but the content of active ingredients in its extract is relatively low. If it is prepared into a prescription, the amount of excipients will be large, which is not conducive to the saving of industrial production costs, and its dosage is relatively large. , inconvenient for patients to use

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A kind of black bone rattan compound extract and its preparation method and application
  • A kind of black bone rattan compound extract and its preparation method and application
  • A kind of black bone rattan compound extract and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Embodiment 1 The preparation of the compound extract of Teng japonica of the present invention

[0032] prescription:

[0033] Black bone vine 50g, Gentiana 20g, Yuanhu 20g

[0034] Preparation:

[0035] (1) Weigh the raw material drug by weight, ultrasonically extract it with 50% v / v ethanol, combine the extracts, recover the ethanol until it has no alcohol smell, and obtain the crude extract;

[0036] (2) Take the crude extract, put it on a D101 macroporous resin column, first elute with water to remove impurities (20 times column volume), then elute with 20 times column volume 20% v / v ethanol, collect the ethanol eluate, reduce After being concentrated under pressure, freeze-dried to obtain the compound extract of Teng Teng.

[0037] For the determination method of total flavonoids and total saponins in the present invention, please refer to the ultraviolet spectrophotometry commonly used in this field; for the detection methods of gentiopicroside, glucoside and t...

Embodiment 2

[0038] Embodiment 2 The preparation of the compound extract of Teng japonica of the present invention

[0039] prescription:

[0040] Black bone vine 50g, Gentiana 20g, Yuanhu 20g

[0041] Preparation:

[0042] (1) Weigh the raw material drug by weight, ultrasonically extract it with 50% v / v ethanol, combine the extracts, recover the ethanol until it has no alcohol smell, and obtain the crude extract;

[0043] (2) Take the crude extract, put it on a D101 macroporous resin column, first elute with water to remove impurities (20 times column volume), and then use 20 times column volume 20%v / v, 40%v / v, 60% Eluted with v / v ethanol, collected 60% ethanol eluate, concentrated under reduced pressure, and freeze-dried to obtain the compound extract of Teng Teng.

Embodiment 3

[0044] Embodiment 3 The preparation of the compound extract of Teng japonica of the present invention

[0045] prescription:

[0046] Black bone vine 50g, Gentiana 20g, Yuanhu 20g

[0047] Preparation:

[0048] (1) Weigh the raw material drug by weight, ultrasonically extract it with 50% v / v ethanol, combine the extracts, recover the ethanol until it has no alcohol smell, and obtain the crude extract;

[0049] (2) Take the crude extract, put it on a D101 macroporous resin column, first elute with water to remove impurities (20 times column volume), and then use 20 times column volume 20%v / v, 40%v / v ethanol to elute successively , collected 40% ethanol eluate, concentrated under reduced pressure, and freeze-dried to obtain the compound extract of S.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention provides a method for preparing a periploca forrestii compound extract. The method comprises the following steps: (1) weighing the following ingredients in parts by weight: 2.5-10 parts of periploca forrestii, 1-4 parts of gentiana macrophylla and 1-4 parts of rhizoma corydalis, after extracting with 40-60 percent v / v ethyl alcohol, merging an extracting solution, recycling ethyl alcohol until the extracting solution does not have an alcohol taste, and then obtaining a crude extract; (2) taking the crude extract to be put into nonpolar macroporous resin columns, washing with water to remove impurities, using 20-60 percent v / v ethyl alcohol to elute, collecting ethyl alcohol elutent, concentrating and drying, and finally obtaining the periploca forrestii compound extract. The invention further provides the periploca forrestii compound extract and the application thereof. Tests show that the periploca forrestii compound extract prepared by the invention has an excellent pharmacological activity on rheumatoid arthritis, and effects of the extracted periploca forrestii are better than other extract parts, so that effective parts of the periploca forrestii is further determined, and guarantees of security monitoring and control of preparations and medicines is provided.

Description

technical field [0001] The present invention relates to a kind of compound extract of Teng chinensis and its preparation method and application. Background technique [0002] Rheumatoid arthritis (RheumatioidArthritis, RA) is an autoimmune deficiency disease characterized by chronic inflammation of the joint synovium. In traditional Chinese medicine, it belongs to the category of "arthritis syndrome". Infection, immune disorders and other factors are related. The incidence of RA in my country is about 0.4%, mostly concentrated in southern provinces. Therapeutic methods include chemical medicine, traditional Chinese medicine, biological agents and other drug treatments and physical therapy. [0003] Black bone vine is the dry root or whole plant of Periploca forrestii Schltr, a plant of the genus PeriplocaLinn. in the family Asclepiasaceae. Commonly used medicine for treating rheumatism and rheumatoid, closed soft tissue injury and other diseases. [0004] Gentiana macrop...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/66A61P29/00A61P19/02
Inventor 张宏胡晓荣李琪刘刚张晓喻
Owner SICHUAN NORMAL UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap