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Culture solution for accelerating rapid growth of microorganisms and using method thereof

A culture solution and microorganism technology, which is applied in the field of culture solution preparation, can solve the problems of long time consumption and low efficiency, and achieve the effects of high efficiency, clean environment and improved efficiency.

Active Publication Date: 2016-11-09
SOUTHEAST UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At the same time, Bacillus pasteurianus is a common bacterium for preparing calcium carbonate, but because it needs nearly 48 hours to reach the stable period under general culture conditions, it is time-consuming and efficient when used in practical projects such as crack repair and concrete cementation. lower disadvantage

Method used

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  • Culture solution for accelerating rapid growth of microorganisms and using method thereof
  • Culture solution for accelerating rapid growth of microorganisms and using method thereof
  • Culture solution for accelerating rapid growth of microorganisms and using method thereof

Examples

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Effect test

Embodiment example 1

[0017] A kind of preparation method of the nutrient solution that promotes the rapid growth of microorganisms, inoculates Bacillus pasteurii (Bacillus pasteurii) in the culture medium that contains multiple nutrients, carries out constant temperature shaking culture under 25~30 ℃, and controls initial pH to be 6 or so, cultivate for 12 to 24 hours, take out part of the bacterial liquid every two hours during the cultivation process, and use a spectrophotometer to measure the absorbance OD 600 , to obtain the growth curve of the strain. In this implementation case, the constant temperature oscillation temperature can be selected at 25, 27 or 30°C, the initial pH value can be controlled at 5.5, 6 or 6.5, and the cultivation time can be selected at 12, 15, 17, 20 or 24 Hour. In addition, in this embodiment, only casein peptone can be added without soybean powder peptone as the main nutrient; in this embodiment, only soybean powder peptone can be added without casein peptone as th...

Embodiment example 2

[0019] Inoculate Bacillus pasteurii (Bacillus pasteurii) in a medium containing various nutrients (the medium composition is shown in Table 1), and carry out constant temperature shaking culture at 25-30°C, and control the initial pH value to about 6 , cultivated for 12 to 24 hours to obtain a turbid culture medium, and after 24 hours, take out the bacterial solution and observe it under a microscope. During the cultivation process, take out part of the bacterial solution every two hours, and use a spectrophotometer to measure the absorbance OD 600 , to get the strain growth curve ( figure 1 ), it was observed that it entered a stable phase in about 18 hours.

[0020] Table 1 Medium composition

[0021]

Embodiment example 3

[0023] Inoculate Bacillus pasteurii (Bacillus pasteurii) in the medium with only casein peptone but no soy peptone as the main nutrient on the basis of the medium in Case 1, and carry out constant temperature shaking culture at 25-30°C, and control The initial pH value is about 6, cultivate for 48 hours, take out part of the bacterial liquid every two hours during the cultivation process, and use a spectrophotometer to measure the absorbance OD 600 , to get the strain growth curve ( figure 2 ), it was observed that it entered a stable phase at about 40 hours.

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Abstract

The invention discloses a culture solution for accelerating rapid growth of microorganisms and a using method thereof. Bacillus pasteurii is inoculated into a culture medium containing various nutrient substances, constant temperature shaking culture is performed at 25-30 DEG C, the initial PH value is controlled to be about 6, culture is performed for 12-24 h, part of bacteria solution is taken out every two days in the culture process, a spectrophotometer is utilized to measure the absorbance OD600, a strain growth curve is obtained, strains enter into a stable stage in about 18 h according to observation, and compared with research by predecessors, the strain growth speed is obviously accelerated. The culture solution for accelerating rapid growth of the microorganisms and the using method have the advantages that substances in the culture solution can be fully utilized, and the culture solution is simple in process, high in efficiency, short in used time, clean in environment and low in cost; the efficiency of microbial induction of calcium carbonate is further improved, and the concrete crack repair time can be shortened.

Description

technical field [0001] The invention relates to a preparation method of a culture solution, in particular to a preparation method of a culture solution for promoting rapid growth of microorganisms. Background technique [0002] Biomineralization is a very common natural phenomenon, and almost every kind of organism can synthesize minerals. Nearly two-thirds of it is calcium ore, and a considerable part has a cementing function. Through their own life activities, they are constantly enzymatically reacting with the surrounding environment medium to gradually form calcium carbonate. Using microorganisms to prepare calcium carbonate has the advantages of clean environment, green environment, simple process and low cost, and this technology has been widely used. At the same time, Bacillus pasteurianus is a commonly used bacterium for preparing calcium carbonate, but because it takes nearly 48 hours to reach the stable period under general culture conditions, it is time-consumin...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/38C12N1/20C12R1/07
CPCC12N1/20C12N1/38
Inventor 缪林昌孙潇昊童天志
Owner SOUTHEAST UNIV
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