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Extraction method of sheep sclera collagen, ophthalmic gel and preparation method thereof

A technology of amniotic scleral collagen and membrane collagen, applied in the field of medicine, can solve the problems of infectious diseases, large molecular weight, etc., and achieve the effects of stable physical and chemical properties, small molecular weight, and no hidden virus

Inactive Publication Date: 2016-06-01
王媛
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] In order to overcome the shortcomings of ocular collagen in the prior art, such as infectious diseases, rejection reactions, and large molecular weight, the present invention provides a method of extracting collagen from sheep sclera to prepare sheep sclera collagen by using enzyme hydrolysis-salt extraction method. Ophthalmic gel, including sheep sclera collagen, boric acid, borax, polyvinyl alcohol, sodium chloride, Tween, water for injection, and preservatives; wherein, the mass fraction of sheep sclera collagen is 80% to 90%, and preservatives The mass fraction of boric acid and borax is 0.01%~2%, the mass fraction of boric acid and borax is 0.5%~2%, the mass fraction of polyvinyl alcohol is 0.5%~2%, the mass fraction of sodium chloride is 0.5~2%, Tween The mass fraction is 0.3% to 2%, and the mass fraction of water for injection is the balance

Method used

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  • Extraction method of sheep sclera collagen, ophthalmic gel and preparation method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0025] Remove other parts of the fresh sheep eyeballs such as the vitreous body, retina and pigmented membrane, separate the sheep sclera, and cut the sheep sclera into thin slices. Take 100g sheep eye sclera fragments, soak and wash them repeatedly with 1000ml of 9g / L normal saline to remove serum and fat, then rinse them with 1000ml distilled water, dry them in a sterile environment, and obtain dry sheep sclera pieces for later use. Take 100 g of sclera fragments and add 10 g of pepsin, that is, the quality of pepsin is 10% of the mass of sheep sclera dry slices, homogenize on a homogenizer, place on a magnetic stirrer and slowly stir for extraction for 24h to 26h, and stir for 24h in this example. Then use a high-speed refrigerated centrifuge to centrifuge for 10 to 30 minutes at a temperature of 0 to 4° C. and a rotational speed of 6,000 revolutions / min, and take the supernatant solution for the first time to obtain a precipitate. Add 50ml of 0.5mol / L acetic acid to the pr...

Embodiment 2

[0035] A sheep sclera collagen ophthalmic gel, comprising sheep sclera collagen, boric acid, borax, polyvinyl alcohol, sodium chloride, Tween, water for injection, and preservatives; wherein, the mass fraction of sheep sclera collagen is 88% , the mass fraction of preservatives is 0.05%, the mass fraction of boric acid and borax is 0.5%, the mass fraction of polyvinyl alcohol is 0.5%, the mass fraction of sodium chloride is 0.5%, the mass fraction of Tween is 0.5%, injection The mass fraction of water is 9.95%. The sheep sclera collagen is extracted from the sclera of sheep eyeballs according to the method for extracting sheep sclera collagen in Example 1.

[0036] The preparation of sheep sclera collagen ophthalmic gel, the method is carried out under aseptic conditions, and its specific preparation steps are:

[0037] 1) getting massfraction is 0.5% boric acid and borax, massfraction is 0.5% polyvinyl alcohol, massfraction is 0.5% sodium chloride, massfraction is 0.5% Tween...

Embodiment 3

[0041] A sheep sclera collagen ophthalmic gel, comprising sheep sclera collagen, boric acid, borax, polyvinyl alcohol, sodium chloride, Tween, water for injection, and preservatives; wherein, the mass fraction of sheep sclera collagen is 87% , the mass fraction of preservatives is 0.08%, the mass fraction of boric acid and borax is 1%, the mass fraction of polyvinyl alcohol is 1%, the mass fraction of sodium chloride is 1%, the mass fraction of Tween is 1%, injection The mass fraction of water is 8.92%. The sheep sclera collagen is extracted from the sclera of sheep eyeballs according to the method for extracting sheep sclera collagen in Example 1.

[0042] The preparation of sheep sclera collagen ophthalmic gel, the method is carried out under aseptic conditions, and its specific preparation steps are:

[0043] 1) getting massfraction is 1% boric acid and borax, massfraction is 1% polyvinyl alcohol, massfraction is 1% sodium chloride, massfraction is 1% Tween, massfraction i...

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Abstract

The invention particularly relates to an extraction method of sheep sclera collagen and a preparation method of ophthalmic gel. An enzyme hydrolysis-salt extraction method is adopted to extract collagen from sheep eye sclera. The invention further provides the ophthalmic gel with the sheep sclera collagen. The ophthalmic gel comprises, by mass, 80-90% of the sheep sclera collagen, 0.01-2% of preservative,0.5-2% of boric acid and borax, 0.5-2% of polyvinyl alcohol, 0.3-2% of Tween, 0.5-2% of sodium chloride and the balance of water for injection. The sheep sclera collagen is similar to human eye sclera collagen in structure and has the advantages of high purity, high tissue compatibility, low molecular weight and low repellency.

Description

technical field [0001] The invention relates to the technical field of medicine, in particular to a method for extracting sheep sclera collagen, an ophthalmic gel and a preparation method thereof. Background technique [0002] Collagen is a macromolecular protein, and its ultrastructural unit is collagen fibers. Due to the different polymerization methods of various types of collagen, they have different functions. Human eye sclera is composed of extracellular matrix and a small amount of cells. Collagen fibers are an important part of extracellular matrix, accounting for about 75% of the dry weight of sclera. The diameter of superficial sclera collagen fibers is 166nm, and the diameter of deep sclera collagen fibers is 100nm. In different parts of the eyeball, the collagen fiber bundles are arranged in different ways: in the front of the eyeball near the limbus, around the optic disc in the back of the eyeball, including the short posterior ciliary artery and the part wher...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K38/01A61K9/06A61P27/10A61K35/30
CPCA61K9/0048A61K9/06A61K35/30A61K38/014A61K47/32
Inventor 王媛
Owner 王媛
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