Antisense oligonucleotide for miR-103-3p and application

An oligonucleotide and nucleotide sequence technology, applied in the field of small molecule biopharmaceuticals, to achieve the effects of low toxicity, low molecular weight, high stability and high cellular uptake rate

Inactive Publication Date: 2016-11-09
叶瑞东
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there is no report that inhibiting the expression of

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  • Antisense oligonucleotide for miR-103-3p and application
  • Antisense oligonucleotide for miR-103-3p and application
  • Antisense oligonucleotide for miR-103-3p and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] 1 method

[0026] 1.1 Experimental animals

[0027] Adult healthy male Sprague-Dawley (SD) rats, 300-350 g, were provided by the Animal Experiment Center of Nanjing General Hospital of Nanjing Military Region, and were reared in a single cage for 3 days before operation to avoid the influence of non-specific factors. 12-hour light / 12-hour dark environment, free to eat and drink.

[0028] 1.2 Experimental method

[0029] 1.2.1 Animal grouping

[0030] SD rats were randomly divided into sham operation group (Sham), antisense oligonucleotide negative control group, and miR-103-3p antisense oligonucleotide group.

[0031] (1) Sham operation group: the thread plug was inserted into the brain, but the blood vessel was not punctured;

[0032] (2) Antisense oligonucleotide negative control group: 24 hours before SAH modeling, according to 0.2nmol / uL (total 5uL), antisense oligonucleotide negative control was administered via the lateral ventricle at 1uL / 10min, the sequence ...

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Abstract

The invention belongs to the field of drug preparation, and particularly relates to antisense oligonucleotide for miR-103-3p. The nucleotide sequence of the antisense oligonucleotide for the miR-103-3p is shown as SEQ ID NO.1. The antisense oligonucleotide for the miR-103-3p can be used for preparing a drug for treating subarachnoid hemorrhage, is low in toxicity and can relieve early brain injuries occurring after subarachnoid hemorrhage.

Description

technical field [0001] The invention belongs to the field of small molecular biomedicine and relates to an antisense oligonucleotide targeting miR-103-3p and its application. Background technique [0002] Subarachnoid hemorrhage (SAH) refers to a stroke in which blood flows into the subarachnoid space after the rupture of blood vessels at the base of the brain or on the surface of the brain, causing corresponding clinical symptoms. It is also called primary subarachnoid hemorrhage. SAH accounts for about 5%-10% of all strokes, with an annual incidence of about 6-20 / 100,000 people. [0003] Common causes of SAH include congenital aneurysm, cerebrovascular malformation, abnormal vascular network disease in the base of the brain (moyamoya disease), dissecting aneurysm, vasculitis, arteriosclerosis in hypertension, etc. Blood enters the subarachnoid space, rapidly disseminates through the cerebrospinal fluid that surrounds the brain and spinal cord, and irritates the meninges c...

Claims

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Application Information

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IPC IPC(8): C12N15/113A61K48/00A61K31/7088A61P9/00A61P9/10A61P25/00
CPCC12N15/113C12N2310/11
Inventor 叶瑞东王刘敏刘新峰
Owner 叶瑞东
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