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Cultivating method of tomato varieties with leaves capable of producing astaxanthin

A cultivation method and astaxanthin technology, applied in the biological field, can solve the problems of cultivation methods and techniques that have not been reported, and achieve the effects of high hardness, strong disease resistance, and strong branching ability

Inactive Publication Date: 2016-11-16
KUNMING INST OF BOTANY - CHINESE ACAD OF SCI
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Problems solved by technology

[0006] 'Yunkang 1', a hybrid of 'Mango-1' and 'CLN2037E', was used as the female parent, and the astaxanthin-producing engineered tomato 'Xiahong 1' was used as the male parent for cross breeding. Thereby obtain a kind of breeding method and technology of the new tomato variety that produces astaxanthin in the leaf and have not yet been reported

Method used

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Embodiment 1

[0022] Hybridization method of tomato variety 'Yunxia 1':

[0023] Using the method of compound crossing, 'Yunkang 1', which was crossed with 'Mango-1' as the female parent and 'CLN2037E' as the male parent, was used as the female parent, and the astaxanthin-producing engineering tomato 'Xiahong 1' was used as the father This hybrid is derived.

[0024] The female parent 'Yunkang No. 1' tomato line is one of the achievements of Yunnan Province's "Tenth Five-Year" key research plan "Research on Breeding of High-quality Tomato Hybrid New Varieties and Supporting Development Technology". Three years of successful breeding. The male parent 'Xiahong 1' is an engineering tomato producing astaxanthin, which was bred in 2009. It was screened and isolated from green algae by Huang Junchao et al. (Huang JC et al., 2013) into plant cells that can catalyze The ketolase and hydroxylase genes of β-carotene and zeaxanthin to synthesize astaxanthin were transferred into wild-type tomato pla...

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Abstract

According to a cultivating method of a new tomato variety 'Yunxia No. 1', based on a fact that a filial generation 'Yunkang No. 1' of 'mango-1' and CLN2037E is used as a female parent, and an astaxanthin production project tomato 'Xiahong No. 1' is used as a male parent, hybridization is performed, leaf astaxanthin is extracted with acetone, and the content of the leaf astaxanthin is detected by a UPLC method. The 'Yunxia No. 1' combines the main excellent properties of the female parent and the main excellent properties of the male parent, and is high in disease resistance and high in astaxanthin yield. The new tomato variety 'Yunxia No. 1' has the main characteristics that plants are in an indeterminate growth type, the height of the plants is 200cm or above, and the growth potential is extremely strong; the new tomato variety 'Yunxia No. 1' is resistant to late blight, early blight and virus diseases; stalks are green to dark red, the branching capacity is high, and the leaf yield is high; the new tomato variety 'Yunxia No. 1' has common leaf types, leafstalks are yellowish green to dark red, veins are yellowish green to dark red, leaves are dark red, and the average content of dry substances namely the astaxanthin reaches 2.02mg / g; calyxes are yellowish green to dark red, radial corollas are yellow to tangerine, anthers are orange to red, and stigmas and persistent fruitlets are red brown; and fruits are orange red, fruit shoulders are claret and oblong, the fruit shape index is 1.07-1.25, fruit surfaces are smooth, the gloss is bright, the pulp is thick, four ventricles exist, the hardness is high, the average weight of single fruits is 120g, and the weight of the largest single fruit can reach 200g or above.

Description

Technical field: [0001] The invention belongs to the field of biotechnology, and in particular relates to a method for cultivating hybrid varieties of tomato plants, and more specifically, relates to 'Yunkang No. 1' which is hybridized with 'Mango-1' as the female parent and 'CLN2037E' as the male parent. In this paper, a tomato three-cross cultivar producing astaxanthin was obtained by crossing the astaxanthin-producing engineering tomato 'Xiahong 1' as the male parent. Background technique: [0002] The female parent of 'Yunkang 1' is 'Mango-1', which is an inbred line selected by the tomato project team of Yunnan Agricultural University College of Landscape Architecture and Horticulture for 3 years. It has high resistance to late blight, early blight and viral diseases, and high yield. Characters, the fruit is orange, and the weight of a single fruit is about 200g. [0003] The male parent of 'Yunkang 1' is 'CLN2037E', an inbred line introduced by the Asian Vegetable Res...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/02
CPCA01H1/02
Inventor 徐海燕熊江何明霞黄俊潮龚洵
Owner KUNMING INST OF BOTANY - CHINESE ACAD OF SCI
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