Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Construction of ratio-type three-dimensional metal-enhanced fluorescence Pb<2+> biosensor

A metal-enhanced fluorescence and biosensor technology, applied in fluorescence/phosphorescence, instruments, scientific instruments, etc., can solve the problems of false positive test results and the accuracy of test results needs to be further improved, and achieves simple production, low cost, The effect of increasing sensitivity

Inactive Publication Date: 2016-12-07
UNIV OF JINAN
View PDF5 Cites 7 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in the fluorescence detection method of a single fluorophore, due to the influence of the environmental instrument factors of the liquid to be tested and the concentration of the fluorophore itself, it will directly lead to false positives in the detection results, and the accuracy of the detection results needs to be further improved.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Construction of ratio-type three-dimensional metal-enhanced fluorescence Pb&lt;2+&gt; biosensor

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] A ratiometric three-dimensional metal-enhanced fluorescent biosensor on Pb 2+ Applications under test:

[0028] (1) Design the hydrophobic wax printing area and hydrophilic working area of ​​the ratiometric 3D metal-reinforced fluorescent paper chip;

[0029] (2) Print the hydrophobic pattern on the paper chip designed in step 1.1 by a wax printer, and then heat the obtained paper chip at 60-150 ºC until the wax melts and soaks the entire paper to form a hydrophobic layer;

[0030] (3) Decahedral silver was grown on the hydrophilic working area of ​​the paper chip obtained in step (2): Mix 1.5 mL 50 mM sodium citrate, 1.5 mL 6% polyvinylpyrrolidone, 0.15 mL 10 mM arginine and 0.6 mL 10 mM silver nitrate was added to 6 mL of secondary water, followed by adding 0.24 mL of 0.1 M freshly prepared sodium borohydride under stirring to prepare silver seeds; 20 µL of silver seeds were added dropwise to the working area and left at room temperature for 45 min , repeated dropwi...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses preparation of a ratio-type three-dimensional metal-enhanced fluorescence Pb<2+> paper chip and application of equipment thereof in Pb<2+> detection. According to the preparation, a hydrophilic working area and a hydrophobic area of a paper chip are prepared by a wax printing technology, and further decahedral silver grows in the working area, the fluorescence intensities of two fluorescence signals are determined by means of layer-by-layer modification, the Pb<2+> is detected portably, reliably and sensitively by the relation curve of variation of two fluorescence intensity ratio over Pb<2+> concentration.

Description

technical field [0001] The invention relates to Pb which is easy to carry, simple to operate, low in cost, high in sensitivity and high in reliability of measurement results. 2+ Analysis and detection technology field, more specifically a ratio-type three-dimensional metal-enhanced fluorescent Pb 2+ Preparation of biosensors. Background technique [0002] Pb 2+ Existing in various resources (such as food, blood, drinking water, industrial waste water, etc.), it is known as one of the most harmful heavy metal ions. It not only causes a series of environmental pollution problems, but also poses a serious threat to human health. Serious threat, especially to children. For example, long-term exposure to Pb-rich 2+ In the environment, it will cause serious damage to the human central nervous system and urinary system, and it will also cause damage to intellectual development through the food chain and drinking water. More serious is Pb 2+ Not biodegradable, trace amounts of...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64
CPCG01N21/64G01N21/6486
Inventor 梁琳琳葛慎光于京华李丽兰飞飞任娜刘海云
Owner UNIV OF JINAN
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com