Construction of ratio-type three-dimensional metal-enhanced fluorescence Pb<2+> biosensor

A metal-enhanced fluorescence and biosensor technology, applied in fluorescence/phosphorescence, instruments, scientific instruments, etc., can solve the problems of false positive test results and the accuracy of test results needs to be further improved, and achieves simple production, low cost, The effect of increasing sensitivity

Inactive Publication Date: 2016-12-07
UNIV OF JINAN
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Problems solved by technology

However, in the fluorescence detection method of a single fluorophore, due to the influence of the environmental instrument factors of the liquid to be tested and the concentration of the fluorophore itself, it will directly lead to false positives in the detection results, and the accuracy of the detection results needs to be further improved.

Method used

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  • Construction of ratio-type three-dimensional metal-enhanced fluorescence Pb&lt;2+&gt; biosensor

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Embodiment 1

[0027] A ratiometric three-dimensional metal-enhanced fluorescent biosensor on Pb 2+ Applications under test:

[0028] (1) Design the hydrophobic wax printing area and hydrophilic working area of ​​the ratiometric 3D metal-reinforced fluorescent paper chip;

[0029] (2) Print the hydrophobic pattern on the paper chip designed in step 1.1 by a wax printer, and then heat the obtained paper chip at 60-150 ºC until the wax melts and soaks the entire paper to form a hydrophobic layer;

[0030] (3) Decahedral silver was grown on the hydrophilic working area of ​​the paper chip obtained in step (2): Mix 1.5 mL 50 mM sodium citrate, 1.5 mL 6% polyvinylpyrrolidone, 0.15 mL 10 mM arginine and 0.6 mL 10 mM silver nitrate was added to 6 mL of secondary water, followed by adding 0.24 mL of 0.1 M freshly prepared sodium borohydride under stirring to prepare silver seeds; 20 µL of silver seeds were added dropwise to the working area and left at room temperature for 45 min , repeated dropwi...

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Abstract

The invention discloses preparation of a ratio-type three-dimensional metal-enhanced fluorescence Pb<2+> paper chip and application of equipment thereof in Pb<2+> detection. According to the preparation, a hydrophilic working area and a hydrophobic area of a paper chip are prepared by a wax printing technology, and further decahedral silver grows in the working area, the fluorescence intensities of two fluorescence signals are determined by means of layer-by-layer modification, the Pb<2+> is detected portably, reliably and sensitively by the relation curve of variation of two fluorescence intensity ratio over Pb<2+> concentration.

Description

technical field [0001] The invention relates to Pb which is easy to carry, simple to operate, low in cost, high in sensitivity and high in reliability of measurement results. 2+ Analysis and detection technology field, more specifically a ratio-type three-dimensional metal-enhanced fluorescent Pb 2+ Preparation of biosensors. Background technique [0002] Pb 2+ Existing in various resources (such as food, blood, drinking water, industrial waste water, etc.), it is known as one of the most harmful heavy metal ions. It not only causes a series of environmental pollution problems, but also poses a serious threat to human health. Serious threat, especially to children. For example, long-term exposure to Pb-rich 2+ In the environment, it will cause serious damage to the human central nervous system and urinary system, and it will also cause damage to intellectual development through the food chain and drinking water. More serious is Pb 2+ Not biodegradable, trace amounts of...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64
CPCG01N21/64G01N21/6486
Inventor 梁琳琳葛慎光于京华李丽兰飞飞任娜刘海云
Owner UNIV OF JINAN
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