Novel anti-flowering-cabbage-downy-mildew biofertilizer and preparation method thereof
A technology for biological fertilizer and downy mildew, which is applied in the field of new biological fertilizers resistant to downy mildew of cabbage and its preparation field, can solve problems such as unsuitable varieties of cabbage, achieve the prevention of downy mildew of cabbage, reduce soil degradation, development-promoting effect
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Embodiment 1
[0038] Inoculate Bacillus subtilis, Bacillus amyloliquefaciens, Paracoccus, photosynthetic bacteria and Streptomyces luteus into the fermentation medium respectively, at 28°C, 150r / min, the fermentation time of Bacillus subtilis and Bacillus amyloliquefaciens is 2 days, and the fermentation time of Bacillus amyloliquefaciens is 2 days. , photosynthetic bacteria and Streptomyces luteus were fermented for 3 days, and then Bacillus subtilis, Bacillus amyloliquefaciens, Paracoccus, photosynthetic bacteria and Streptomyces luteus were mixed according to the effective number ratio of 3.5:3.0:0.5:0.8:1.2 , to prepare a composite microbial agent, wherein the total number of effective viable bacteria in the fermentation broth is about 5.6×109 CFU / ml.
[0039] Mix 250kg of light bamboo leaves, 650kg of chicken manure, 50kg of biochar, and 50kg of bamboo chips. Tank fermentation is adopted, when the temperature reaches 60°C, the compost is turned over, and the high temperature period (a...
Embodiment 2
[0042] Inoculate Bacillus subtilis, Bacillus amyloliquefaciens, Paracoccus, photosynthetic bacteria and Streptomyces luteus into the fermentation medium respectively, at 30°C, 180r / min, the fermentation time of Bacillus subtilis and Bacillus amyloliquefaciens is 2 days, and the fermentation time of Bacillus amyloliquefaciens is 2 days. , photosynthetic bacteria and Streptomyces flavinus were fermented for 3 days, and then Bacillus subtilis, Bacillus amyloliquefaciens, Paracoccus, photosynthetic bacteria and Streptomyces luteus were fermented according to the effective number ratio of 5.0: 3.5: 0.8: 1.0: 1.5 mixed to prepare a composite microbial agent, wherein the total effective number of viable bacteria in the fermentation broth was 1.7×10 10 CFU / ml.
[0043] Mix 400 kg of light bamboo leaves, 550 kg of chicken manure, 30 kg of biochar, and 20 kg of bamboo chips. Tank fermentation is adopted, when the temperature reaches 60°C, the compost is turned over, and the high tempe...
Embodiment 3
[0045] Inoculate Bacillus subtilis, Bacillus amyloliquefaciens, Paracoccus, photosynthetic bacteria and Streptomyces luteus into the fermentation medium respectively, at 29°C, 160r / min, the fermentation time of Bacillus subtilis and Bacillus amyloliquefaciens is 2 days, and the fermentation time of Paracoccus , photosynthetic bacteria and Streptomyces flavinus were fermented for 3 days, and then Bacillus subtilis, Bacillus amyloliquefaciens, Paracoccus, photosynthetic bacteria and Streptomyces luteus were 4.0: 3.2: 0.6: 0.9: 1.3 according to the ratio of effective viable bacteria mixed to prepare a composite microbial agent, wherein the effective number of viable bacteria in the final fermentation broth was 8.3×10 9 CFU / ml.
[0046] Mix 350 kg of light bamboo leaves, 600 kg of chicken manure, 10 kg of biochar, and 40 kg of bamboo chips. Tank fermentation is adopted, when the temperature reaches 60°C, the compost is turned over, and the high temperature period (above 50°C) is...
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