A method for quail sex identification based on pcr technology

Abstract

The invention discloses a method for identifying the sex of quail based on PCR technology, and belongs to the technical field of biological detection and identification. The two pairs of PCR primers used for PCR identification of the present invention include a pair of microsatellite primers and a pair of internal reference gene (β-actin) primers. The PCR identification method is: using the blood sample genomic DNA of the young quail to be tested as a template, using the quail sex Identification Use PCR primers for PCR amplification, and then use agarose gel electrophoresis to detect the PCR amplification product. When the amplification product only has a 259 bp specific amplification band, it is determined to be a male quail. When the amplification product appears 259 bp The specific amplification band of 114 bp and the specific amplification band of 114 bp were judged as female quail. The method has the advantages of simple and convenient operation, capable of sex identification at the hatching, rapid identification, accurate results, etc., and avoids the disadvantages of long time consumption, high feeding cost, error-prone, etc. of the traditional method.

Description

technical field [0001] The invention belongs to the technical field of biological detection and identification, and in particular relates to a method for quail sex identification based on PCR technology, which can be applied to early sex identification of baby quails. Background technique [0002] Quail is a special economic poultry and the smallest poultry breed, which has extremely high edible value and therapeutic effect. Quail eggs are rich in nutrition, high in protein and low in cholesterol. It can be seen that the breeding of quail for eggs has a good market prospect. Since the weight of quail eggs hatched is only about 7 grams, usually commercial egg substitutes use quails to distinguish males and females by feather color; and parental quails have the same coat color, so males and females cannot be distinguished when hatching, and generally have to wait for the quails to mature. It is not until about 30 days old that males and females can be distinguished by body sh...

AI Technical Summary

Problems solved by technology

The advantage of this method is that the cost is low, and it is widely used under the existing identification conditions. The disadvantage is that the operation is cumbersome, the time is long, and it is easy to cause pollution.

In recent years, some scholars have redesigned primers to distinguish ZW bands by using the different melting temperatures of CHD-W and CHD-Z amplification products, but the amplification reactions of CHD-Z and CHD-W were carried out in two PCR reaction tubes respectively. This not only increases the economic cost, but also has low practicability, and on the other hand, misjudgment may occur; another report shows that CHD-W and CHD-Z are amplified with the same primers in the same tube, and the amplified fragments are used to Z and W have 3 SNPs, and the melting temperature of their amplified products is different to distinguish. This method only uses a pair of primers, and the sequence and size of the generated fragments are very close, so they cannot be distinguished by gel electrophoresis; the disadvantages are: Users who do not have a melting curve analyzer cannot use it, and lack a simple and effective method to verify the results

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