Primer, kit and detection method for detecting deafness genetic risk of children

Abstract

The invention discloses a primer and a kit for detecting the deafness genetic risk of children and an operation method thereof. The method comprises the following steps: (1) extracting a DNA template, and extracting DNA of a genome of oral epithelial cells; (2) performing a PCR amplification reaction with the total volume of a reaction system being 25 mu L at 94 DEG C for 12 minutes, performing the reaction at 94 DEG C for 30 seconds, at 60 DEG C for 30 seconds and at 72 DEG C for 30 seconds for 30 circulations, and performing PCR amplification reaction at 72 DEG C for 10 minutes; (3) purifying a PCR product at 37 DEG C for 15 minutes and at 72 DEG C for 20 seconds; and (4) performing a DNA sequencing reaction at 98 DEG C for 2 seconds by using a DNA sequencing primer GCGTTTGGTCCTAGCCTTT, and performing the reaction at 96 DEG C for 30 seconds, at 55 DEG C for 30 seconds and 60 DEG C for 4 minutes for 25 circulations. The detection method has the advantages of high accuracy and simple operation, and has a huge market popularization and application value.

Description

technical field [0001] The invention relates to the fields of molecular biology and medicine, in particular to a primer, a kit and a detection method for detecting the genetic risk of deafness in children. Background technique [0002] At present, 20,000-30,000 deaf children are born in my country every year, 50% of which are caused by genetic factors. Deafness caused by adverse drug reactions is the main cause of congenital deafness in newborns and acquired deafness in adults. [0003] What is damaged by drug-induced deafness is not the sound conduction system of the outer ear and middle ear, that is, non-conductive deafness, but the hair cells of the cochlea, the most important and most vulnerable part of the perception of sound, are damaged by drug poison. Hair cells are the peripheral receptors of the auditory nerve. Under normal circumstances, the hair cells convert sound energy into bioelectric impulses, which are transmitted to the auditory nerve and input to the bra...

AI Technical Summary

Problems solved by technology

However, in patients with deafness caused by aminoglycoside antibiotics, some patients are particularly sensitive to toxicity, and a small dose or even a single dose of the drug may cause deafness, and there are individual differences

Generally speaking, it is relatively safe to use this type of drug at a normal dose, but some patients have family genetic susceptibility and individual differences to this type of drug. Early or severe ototoxicity