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Preparation and application method of bacterial agent for hexavalent chromium pollution control in watershed

A technology for pollution control and hexavalent chromium, applied in the field of chromium pollution control, can solve problems such as hereditary genetic defects, allergies, and high toxicity of hexavalent chromium, and achieve strong removal of hexavalent chromium and high reduction efficiency

Inactive Publication Date: 2020-01-07
路域生态工程有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Hexavalent chromium is highly toxic. Hexavalent chromium is a poisonous substance for swallowing / inhalation. Skin contact may cause allergies; it is more likely to cause genetic defects, inhalation may cause cancer, and has a persistent danger to the environment.

Method used

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  • Preparation and application method of bacterial agent for hexavalent chromium pollution control in watershed
  • Preparation and application method of bacterial agent for hexavalent chromium pollution control in watershed
  • Preparation and application method of bacterial agent for hexavalent chromium pollution control in watershed

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Experimental program
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Effect test

preparation example Construction

[0038] The preparation method of the strain includes the following steps:

[0039] Step 1. Prepare fermentation raw materials: According to the mass percentage of water, prepare fermentation raw materials, liquid molasses 5.0%, industrial glucose 1.0%, industrial yeast powder 0.5%, bran powder 3.0% (the crushing requirement is less than 100 mesh), malt extract 1.0 %, dipotassium hydrogen phosphate 0.3%, sodium dihydrogen phosphate 0.1%, ammonium sulfate 0.5%, magnesium sulfate 0.05%;

[0040] Step 2. Sterilization: Put the raw materials in step 1 into the fermentation tank for sterilization, the sterilization temperature is 121°C, and the sterilization time is 0.5 hours;

[0041] Step 3. Fermentation: After sterilization, ferment the fermentor containing the fermentation raw materials; the fermentation temperature is controlled at 30-32°C, and the fermentation time is 96 hours. When the fermentation is carried out, the air:liquid aeration ratio of the aeration is 0~ 24 hours is 1:0....

Embodiment 1

[0047] Example 1. Test basis

[0048] Determination method of hexavalent chromium in sewage:

[0049] Water quality-Determination of hexavalent chromium-Diphenyl carbonate dihydrazine spectrophotometric method GB7467-1987

[0050] Determination method of total chromium in sewage:

[0051] Water quality. Determination of total chromium. Potassium permanganate oxidation-diphenyl carbonate dihydrazine spectrophotometric method GB7466-1987

Embodiment 2

[0052] Example 2 The specific steps for the product of the present invention to treat high-concentration hexavalent chromium heavy metal polluted water are as follows:

[0053] The product produced by the above method was added to the hexavalent chromium polluted water 1, and the polluted water 1 was tested to contain hexavalent chromium at a concentration of 1790 mg / L. The volume ratio of the product to the polluted water is 2.5:100 (L / L), the pH of the polluted water is adjusted to 7.0, the temperature of the polluted water is controlled to 30°C, the stirring speed is 100r / min, and the processing interval is 24h. The concentration of chromium and hexavalent chromium. After the Cr(Ⅵ) concentration in the culture medium was lower than the detection limit, sampling was stopped.

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Abstract

The invention discloses a production method of Streptomyces puniceus strain LYzhlm (with the preservation number of CGMCC No.11773) inoculant, and a use method using the above product to treat hexavalent chromium polluted water. Experiments using the product to treat hexavalent chromium polluted water show that the product can reduce the concentration of hexavalent chromium in sewage to 0.08 mg / L from 1790 mg / L, so hexavalent chromium discharge standards are completely reached, and the removal rate is 100%. The invention also discloses a production technology of the above strain, and conditions and a method for treating the hexavalent chromium polluted water. The use method using the product to treat hexavalent chromium polluted water has the advantages of simple process, avoiding of secondary pollution generated in chemical methods, convenience in use, and wide market prospect.

Description

Technical field [0001] The invention relates to the technical field of chromium pollution treatment, in particular to a method for preparing and using a bacterial agent for treatment of hexavalent chromium pollution in a watershed. Background technique [0002] Chromium is a silver-white hard metal with divalent, trivalent and hexavalent compounds. Chromium has a wide range of industrial uses, including metal processing, electroplating, and tanning industries. The waste water and waste gas discharged from these industries are the main sources of pollution in the environment of the river basin. Hexavalent chromium is highly toxic. Hexavalent chromium is a very toxic substance by swallowing / inhalation. Skin contact may cause allergies; it is more likely to cause hereditary genetic defects. Inhalation may cause cancer and has a lasting risk to the environment. But these are the characteristics of hexavalent chromium, chromium metal, trivalent or tetravalent chromium does not have t...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C02F3/34C12R1/46C02F101/22
CPCC02F3/348C02F2101/22C12N1/20
Inventor 孙莹赵英王新民
Owner 路域生态工程有限公司