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Method for simultaneously detecting vitamin A and 25-hydroxyl vitamin D in blood

A hydroxyvitamin and vitamin technology, applied in the field of vitamin detection, can solve the problems of vitamin content change, long detection process time, poor method specificity, etc., and achieve the effects of high specificity, short instrument analysis time, high accuracy and sensitivity

Active Publication Date: 2017-02-22
BEIJING HARMONY HEALTH MEDICAL DIAGNOSTICS CO LTD
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Problems solved by technology

In the prior art, the detection of vitamins mostly adopts the detection method combined with multi-wavelength absorption or joint absorption or fluorescence absorption after separation by high performance liquid chromatography (HPLC). Dealing with complex problems that lead to changes in vitamin content during sample processing, long analysis time, poor specificity of the method, and long time in the entire detection process, and the simultaneous detection of vitamin A and 25-hydroxyvitamin D in blood by liquid chromatography-tandem mass spectrometry There are very few literatures few

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  • Method for simultaneously detecting vitamin A and 25-hydroxyl vitamin D in blood
  • Method for simultaneously detecting vitamin A and 25-hydroxyl vitamin D in blood
  • Method for simultaneously detecting vitamin A and 25-hydroxyl vitamin D in blood

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Embodiment Construction

[0045] A method for simultaneously detecting vitamin A and 25-hydroxyvitamin D in blood, comprising the following steps:

[0046] (1) Centrifuge the whole blood sample at a speed of 3500r / min and a centrifugation time of 10min, take the supernatant to obtain serum, and store the serum at 4°C-8°C in a refrigerated and dark condition (within 24h) until analysis Store it in the dark at -20°C (avoid repeated freezing and thawing) (within 72 hours) until analysis, or store it in the dark at -80°C (avoiding repeated freezing and thawing) for 30 hours within days) until ready for analysis;

[0047] (2) Calibration of standard solution

[0048] Mix 200ul of the mixed standard working solution of seven different concentrations of standards with 10ul of the internal standard working solution and vortex mix at 1500r / min for 30s, mix well, and make a standard curve series of solutions; the standard curve series of solutions are prepared before detection A UV spectrophotometer must be pe...

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Abstract

The invention discloses a method for simultaneously detecting vitamin A and 25-hydroxyl vitamin D in blood. The method comprises the following steps: firstly, centrifuging a whole blood sample, and taking supernate to obtain serum or plasma for later use; secondly, calibrating a standard solution; thirdly, pretreating a sample; fourthly, taking 80mu L of to-be-detected supernate sample treated in the third step, putting the to-be-detected supernate sample into an automatic sample feeding bottle, and analyzing by liquid chromatography-tandem mass spectrometry; meanwhile, quantitatively detecting the contents of the vitamin A and the 25-hydroxyl vitamin D in the blood. The method for simultaneously detecting the vitamin A and the 25-hydroxyl vitamin D in the blood, disclosed by the invention, has the advantages of high specificity, high accuracy, high flexibility and short analysis time.

Description

technical field [0001] The invention relates to the technical field of vitamin detection, in particular to a method for simultaneously detecting vitamin A and 25-hydroxyvitamin D in blood. Background technique [0002] Vitamin A (VA) is a fat-soluble alcohol substance with various molecular forms. It is the first vitamin to be discovered. Among them, vitamin A1 (VA1) mainly exists in animal liver, blood and retina of the eye, also known as retinol. , melting point 64°C, molecular formula C 20 h 30 O, see chemical structural formula (1); vitamin A2 (VA2) mainly exists in freshwater fish, its melting point is only 17-19°C, and its molecular formula is C 20 h 28 O, also known as retinal. Vitamin A is a component of rhodopsin that senses weak light in visual cells. The lack of vitamin A in the human body affects the ability to adapt to the dark, such as children with stunted growth, dry skin, dry eye disease, night blindness, and age spots. [0003] [0004] Vitamin D (v...

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Application Information

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IPC IPC(8): G01N30/02G01N30/88
CPCG01N30/02G01N30/88G01N2030/8822
Inventor 倪君君李玮
Owner BEIJING HARMONY HEALTH MEDICAL DIAGNOSTICS CO LTD
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