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Improved formula of TSA culture medium

A medium and formula technology, applied in the field of bioengineering, can solve problems such as the inability of anaerobic microorganisms to grow well, and achieve the effect of maintaining osmotic pressure and repairing damaged bacteria

Inactive Publication Date: 2017-03-08
哈尔滨亿隆科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] The existing TSA medium is basically composed of tryptone, soytone, sodium chloride, and agar. Although it is rich in nutrients, it can only cultivate aerobic microorganisms because of its high oxidation-reduction potential. not growing well

Method used

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  • Improved formula of TSA culture medium

Examples

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Effect test

Embodiment 1

[0017] The improved formulation of the TSA medium provided in this example consists of tryptone, soytone, liver digestate, yeast extract, sodium chloride, sodium thioglycolate, glucose, dipotassium hydrogen phosphate, and agar, wherein: per 1000ml The culture medium contains 17g of tryptone, 3g of soytone, 2g of liver digestate, 2g of yeast extract, 4g of sodium chloride, 1g of sodium thioglycolate, 2.5g of glucose, 2g of dipotassium hydrogen phosphate, 15g of agar, pH = 7.3±0.2.

[0018] The preparation steps of the above medium are as follows: mix tryptone, soybean peptone, liver digestate, yeast extract, sodium chloride, sodium thioglycolate, glucose, dipotassium hydrogen phosphate, and agar according to the proportion, add purified water to volume Heat to dissolve and adjust the pH value to 7.2±0.2, sterilize at 121°C for 15 minutes, cool down to 50°C, perfuse the plate, condense to obtain the finished medium, and set aside.

[0019] The new TSA medium prepared with the a...

Embodiment 2

[0026] The difference between this example and Example 1 is: in every 1000ml medium, tryptone 16g, soytone 4g, liver digestate 3g, yeast extract 4g, sodium chloride 3g, sodium thioglycolate 0.5g, glucose 1.5g g, 2.5 g of dipotassium hydrogen phosphate, 18 g of agar, pH=7.3±0.2.

Embodiment 3

[0028] The difference between this example and Example 1 is that every 1000ml of medium contains tryptone 18g, soytone 2g, liver digestate 4g, yeast extract 3g, sodium chloride 5g, sodium thioglycolate 1.5g, glucose 3.5g, 1.5g of dipotassium hydrogen phosphate, 12g of agar, pH=7.3±0.2.

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Abstract

The invention discloses an improved formula of a TSA culture medium. The improved formula of the TSA culture medium consists of casein tryptone, soybean peptone, liver digests, a yeast extract, sodium chloride, sodium thioglycolate, glucose, dipotassium hydrogen phosphate and agar, wherein each 1000ml of the culture medium consists of 15-20g of the casein tryptone, 1-5g of the soybean peptone, 1-5g of the liver digests, 1-5g of the yeast extract, 2-6g of the sodium chloride, 0.5-2g of the sodium thioglycolate, 1-4g of the glucose, 1-3g of the dipotassium hydrogen phosphate and 10-20g of the agar, and the pH value of the culture medium ranges from 7.1 to 7.5. The improved formula of the TSA culture medium provided by the invention can offer comprehensive nutrition, maintain bacterium osmotic pressure and repair damaged bacteria; and on the basis of avoiding influence on an existing performance, namely guaranteeing the culture of aerobes, the improved formula is applicable to the culture of most anaerobes as well.

Description

technical field [0001] The invention belongs to the technical field of bioengineering, and relates to an improved formula of a TSA medium, in particular to an improved formula of a TSA medium which can meet the overall needs of common bacteria and most anaerobic bacteria. Background technique [0002] The existing TSA medium is basically composed of tryptone, soytone, sodium chloride, and agar. Although it is rich in nutrients, it can only cultivate aerobic microorganisms because of its high oxidation-reduction potential. Does not grow well. Contents of the invention [0003] In order to broaden the cultivation range of TSA and meet the growth conditions of most aerobic microorganisms and anaerobic microorganisms, the present invention modifies part of the TSA formula and provides an improved formula of TSA medium. [0004] The purpose of the present invention is achieved through the following technical solutions: [0005] An improved formula of TSA medium, which consist...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/16C12R1/125C12R1/46C12R1/145C12R1/725
CPCC12N1/16C12N1/20
Inventor 李娜
Owner 哈尔滨亿隆科技有限公司
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