Inhibitory effect of celery root exudate concentrate on Ralstorinia solanacearum and extraction method for celery root exudate concentrate

A tomato bacterial wilt fungus and root exudate technology, applied in botany equipment and methods, chemicals for biological control, animal repellants, etc., can solve the problems of severe disease and high incidence of bacterial wilt, and achieve cost-effective Inexpensive and easy to use

Active Publication Date: 2017-03-15
ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The present invention aims to solve the problem of severe disease occurrence in the existing tomato planting industry, especially the high incidence of bacterial wilt, and provides a method that is easy to use, low in cost, and capable of significantly inhibiting the biological activity of bacterial wilt in tomato soil.

Method used

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  • Inhibitory effect of celery root exudate concentrate on Ralstorinia solanacearum and extraction method for celery root exudate concentrate
  • Inhibitory effect of celery root exudate concentrate on Ralstorinia solanacearum and extraction method for celery root exudate concentrate
  • Inhibitory effect of celery root exudate concentrate on Ralstorinia solanacearum and extraction method for celery root exudate concentrate

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Experimental program
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Effect test

Embodiment 1

[0020] Embodiment 1, the inhibitory effect of celery root exudates on tomato Ralstonia solanacearum, implementation method is as follows:

[0021] Plant celery 'Jinnan Shiqin No. 1' in an artificial climate chamber (12h light / 12h dark, day and night temperature 25°C / 20°C), sow 20 celery seeds in each pot, and pull out the roots when the celery grows to a height of 30-40cm , collect the remaining substrate in the pot for use, in addition, plant the tomato wild-type strain 'Alisa' in the plug tray of the artificial climate chamber, and when the tomato plant grows to 5 leaves and 1 heart, 3 plants are transplanted into the normal substrate (control) , 4 strains were transplanted in the matrix (treatment) that had planted celery, added tomato solanacearum Pseudomonas solanacearum (race1) 100ml (OD600=1.0) in the matrix of treatment and contrast simultaneously, observed under the high-humidity environment; The result shows that 4 Days later, all three tomato plants in the control g...

Embodiment 2

[0022] Embodiment 2, the inhibitory effect of celery root exudate concentrate on tomato Ralstonia solanacearum, implementation method is as follows:

[0023] Celery 'Jinnan Shiqin No. 1' was planted in an artificial climate chamber (12h light / 12h dark, day and night temperature 25°C / 20°C), and 20 celery seeds were sown in each pot. When the celery grows to a height of 30-40cm, pull out the root carefully from the pot, rinse thoroughly to remove the soil attached to the root, and avoid root damage; take 5 healthy celery plants, put the root system into a light-proof triangular flask, and use 1 / 2 Hoagland After 3 days of nutrient solution hydroponics (to make the celery root system grow vigorously), transfer to a light-proof Erlenmeyer flask containing 200ml sterile water to cultivate and collect the concentrated solution of root exudates for use; in addition, plant tomato in the artificial climate chamber plug tray Wild-type strain 'Alisa', when the tomato plants grew to 5 leav...

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Abstract

The invention discloses inhibitory effect of a celery root exudate concentrate on Ralstorinia solanacearum and an extraction method for the celery root exudate concentrate. The extraction method comprises the following steps: 1) cultivating celery with a nutritive bowl; 2) in 45 d, selecting five celery strains with good growth vigor and subjecting the roots of the celery strains to hydroponic culture with a nutrient solution in a lucifugal conical flask; 3) carrying out hydroponic culture for 3 d until the roots of the celery strains vigorously grow, transferring the roots the celery strains to another lucifugal conical flask with 200 ml of sterile water for culture; 4) after culture for 2 d, carrying out rapid filtering and collection of a nutrient solution in the conical flask, and subjecting the nutrient solution to freezing and evaporation at -50 DEG C until the nutrient solution is concentrated to a volume accounting for 1 / 20 of the original volume of the nutrient solution so as to obtain the concentrate of celery root exudates; and 5) filtering the concentrate of celery root exudates with a filter head with a size of 0.22 [mu]m and then carrying out aseptic preservation at 4 DEG C. The celery root exudate concentrate is convenient to use and low in cost and can substantially inhibit the biological activity of Ralstorinia solanacearum in continuous tomato cropping soil.

Description

technical field [0001] The invention relates to the technical field of vegetable disease-resistant cultivation, in particular to a celery root exudate concentrate that inhibits Ralstonia solanacearum of tomato and an extraction method thereof. Background technique [0002] Tomato (Lycopersicon esculentum Mill.) originated in Mexico, South America. It has many varieties, wide adaptability, high yield, rich nutrition and wide application. It is one of the important edible vegetable crops in the world; One of the factors is the epidemic hazard of the disease. Bacterial wilt of crops is a worldwide bacterial disease, which is particularly harmful to tomato; tomato bacterial wilt is increasingly serious in the south of the Yangtze River in my country, especially in South China, causing Tomato planting has experienced a decrease in yield or even no harvest; with the rapid expansion of the scale of protected tomato cultivation in my country, the control of tomato bacterial wilt (Pseu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N65/10A01P1/00
CPCA01N65/00A01N65/10
Inventor 程远万红建叶青静阮美颖姚祝平李志邈王荣青周国治杨悦俭
Owner ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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