Broad-spectrum detection method of carcinogenic pathogenic microorganisms

Abstract

The invention relates to the field of detection and identification of pathogenic microorganism preparation, especially to a kit for detecting DNA / RNA of carcinogenic pathogenic microorganisms in biological samples. The kit contains a synthesized polynucleotide reagent. The polynucleotide contains primer sequences SEQ ID NO.1-SEQ ID NO.70 of forward and reverse primers of a specific gene sequence of DNA / RNA carcinogenic pathogenic microorganisms, base expanded probe sequences SEQ ID NO. 75-SEQ ID NO.109, DNA / RNA quality control primer sequences SEQ ID NO.71-SEQ ID NO. 74, and probe sequences SEQ ID NO.110 and SEQ ID NO.111.

Description

technical field [0001] The invention relates to the field of detection and identification of pathogenic microorganism preparations, in particular to a method for broad-spectrum detection of carcinogenic pathogenic microorganisms. Background technique [0002] In December 2010, Patrick S. Moore (senior virologist at the University of Pittsburgh, member of the American Academy of Sciences) and his wife, Professor Yuan Chang, were the co-discoverers of Kaposi sarcoma virus (KSHV) and Merkel cell polyoma virus (MCV) Co-authored "Why Do Viruses Cause Cancer? The First Century of Human Tumor Virology". Since Francis Peyton Rous (Francis Peyton Rous) first discovered poultry sarcoma virus in 1911, he epoch-makingly proposed that "cancerous tumors are caused by viruses" and became the first "tumor virus" person, and won the Nobel Prize in 1966 Physiology or Medicine Prize. Since then, viruses have become the core of modern tumor research. In 1964, the human tumor virus Epstein-Bar...

AI Technical Summary

Problems solved by technology

Not only the detection cost is high and the cycle is long, but also the possibility of sample deterioration and contamination is high

For organ transplantation and blood products, as well as the increasing incidence of infectious cancers caused by biological carcinogens, there is a lack of powerful, comprehensive and accurate detection methods

[0026] The ELISA immune reaction detection method using conventional antigens and antibodies can only represent whether the patient has ever been infected with these pathogens

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