Aspergillus niger and its culture method, bacterial agent and application

A cultivation method, the technology of Aspergillus niger, applied in the field of microorganisms, can solve the problems of slow decomposition speed, influence on promotion, influence on the rooting of successor crops, etc., and achieve the effect of rapid decay, high economic benefit and social benefit

Active Publication Date: 2019-09-03
粮华生物科技(北京)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the slow decomposition rate of straw after returning to the field, it affects the rooting of subsequent crops, and a series of problems such as microorganisms and crops compete for nutrients during degradation, resulting in nitrogen starvation of crops, have affected the promotion of this technology.

Method used

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  • Aspergillus niger and its culture method, bacterial agent and application
  • Aspergillus niger and its culture method, bacterial agent and application
  • Aspergillus niger and its culture method, bacterial agent and application

Examples

Experimental program
Comparison scheme
Effect test

preparation example

[0063] This preparation example is used to prepare the fungal seed liquid used in the following examples.

[0064] The above-mentioned fungal liquid culture medium was sterilized at 115°C for 20 minutes, and cooled to room temperature. Insert the bacterium solution of 2 volume %-5 volume % Aspergillus niger strains into 200 mL of the above-mentioned sterilized fungal liquid medium, and cultivate it for 5 days at 150-240 rpm and 25-35 ° C under the culture conditions to make the spores amount up to 10 7 cells / mL to obtain the fungal seed solution.

Embodiment 1

[0066] This example is used to illustrate the straw solid-state fermentation degradation ability of Aspergillus niger of the present invention.

[0067] Weigh 16g of rice straw, add it to 220mL solid-state fermentation inorganic salt medium, then autoclave at 121°C for 15min, stir evenly, pour it into 8 sterile petri dishes on average, cool to room temperature, pour each Add 1 mL of the fungal seed liquid prepared in the above preparation example into the petri dish. Then place the petri dish in a 28°C incubator for culture, and take out a petri dish on the 3rd day, 6th day, 9th day, 12th day and 15th day after culture, and measure the sample after sterilizing and drying Weight loss rate, and cellulose, hemicellulose and lignin degradation rate. The results are shown in Table 1.

[0068] The fermentation product on the 15th day after cultivation was dried, and the surface structure of the straw was observed with a scanning electron microscope. The results were as follows: ...

Embodiment 2

[0079] This example is used to illustrate the ability of the Aspergillus niger of the present invention to degrade lignin.

[0080] Test group: take 100mL organic solvent extraction wheat straw lignin inorganic salt liquid medium, autoclave at 121°C for 15min, cool to room temperature, insert 1mL fungal seed solution prepared in the above preparation example, and then sterilize at 170rpm and 30 Cultivate for 5 days at ℃.

[0081] Blank control group: except that 1 mL of fungal seed liquid was replaced by 1 mL of fungal liquid culture medium, other operating conditions were the same as those of the test group.

[0082]Take out a little of the five-day cultured liquid of the test group and the blank control group, centrifuge at 12,000 rpm for 10 min, and get the supernatant. The absorbance of the supernatant was measured at 280 nm by a UV spectrophotometer. Among them, the absorbance value of the test group was 0.313, and the absorbance value of the blank control group was 0.6...

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Abstract

The present invention relates to the field of microorganisms, and specifically discloses a strain of Aspergillus niger, wherein the preservation number is CGMCC No.11113. The present invention further discloses a culture method of the Aspergillus niger, and a bacterial agent containing the Aspergillus niger. The invention further discloses applications of the Aspergillus niger and the bacterial agent in straw degradation. With the application of the Aspergillus niger to degrade the straw in soil and water bodies, the straws can be rapidly corroded and degraded, a lot of nitrogen, potassium and other nutrition elements required by crop growth can be released, a series of problems that the slow straw decomposition rate affects the subsequent crop rooting, the microorganisms used during the degradation contest the nutrients with crops so as to cause the nitrogen starvation of crops, and the like in the existing technology for reutilization of the straws in the field are solved, and the high economic and social benefits are provided.

Description

technical field [0001] The invention belongs to the field of microorganisms, and relates to a strain of Aspergillus niger, in particular to a strain of Aspergillus niger with the function of degrading stalks, a method for cultivating the Aspergillus niger, a bacterial agent containing the Aspergillus niger as an active ingredient, and their ability to degrade stalks Application in straw. Background technique [0002] Crop straw is a renewable biomass resource that contains abundant energy. At present, the amount of crop straw produced in my country is huge every year, and the amount of straw collected can reach 600-800 million tons. Due to the lack of corresponding technology, a large number of crop straws are burned randomly, which not only seriously pollutes the environment, but also causes a huge waste of biomass resources. Straw returning to the field is the most convenient and practical technology with the largest utilization of straw. It mechanically pulverizes the f...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14C05F11/08C12R1/685
Inventor 林森任立伟过钰梁王培红
Owner 粮华生物科技(北京)有限公司
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