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Method for evaluating existence of antibiotics in meat product

A technology of antibiotics and products, which is applied in the direction of biochemical equipment and methods, and the determination/inspection of microorganisms, can solve the problems of time-consuming and labor-intensive detection, and achieve the effect of improving reliability

Inactive Publication Date: 2017-05-10
深圳市百澳飞生物技术有限公司
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AI Technical Summary

Problems solved by technology

[0005] Most of the existing antibiotic detection is for specific types of detection, and there are many types of antibiotics, and the detection is time-consuming and labor-intensive. Therefore, the existing detection methods are prone to neglected types and cannot avoid antibiotic residues

Method used

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  • Method for evaluating existence of antibiotics in meat product
  • Method for evaluating existence of antibiotics in meat product

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Experimental program
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Effect test

Embodiment

[0029] 1. Fecal source and in vitro chemostat model construction

[0030] Select 4 healthy volunteers (2 males and 2 females, aged 22-28 years old) who have not used antibacterial drugs for more than 6 months, have no gastrointestinal diseases, and have no abnormal eating habits. The feces were put in sterile anaerobic bags (nitrogen 85%, hydrogen 5%, carbon dioxide 10%), 10% glycerin was added as an antifreeze protection agent, vortexed and shaken to mix well, and stored at -80°C.

[0031] Refer to the Chinese patent ZL 200710028346.2 to assemble the in vitro chemostat, inject 500ml of sterilized intestinal flora culture medium into the fermenter of the in vitro chemostat model, and seal it with wax, so that the whole system is in a sterile and airtight environment. Turn on the peristaltic pump connected to the fresh medium for 35ml.h -1 Flow rate operation, conveying fresh medium to continuously replenish nutrients, and waste liquid is removed at the same flow rate to ensur...

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Abstract

The invention provides a method or evaluating existence of antibiotics in a meat product. Firstly, an in-vitro chemostat model of human intestinal flora is constructed, and the to-be-evaluated meat product is added into the model; and the intestinal flora conditions of the in-vitro chemostat model, after and before the meat product is added, are obtained through a 16S rDNA sequencing method, and if the intestinal flora has no fluctuation change, the meat product does not contain the antibiotics, and otherwise, the meat product contains the antibiotics. The evaluating method combines the in-vitro chemostat model and the metagenome technology, is not limited by the variety of the antibiotics, improves the reliability, the accuracy and the sensitivity of detection of vitamin residues, and provides an excellent model and an advanced detecting technique for the establishment of a more scientific and comprehensive antibiotics residue safety assessment system for antibiotics-free products.

Description

technical field [0001] The invention relates to the field of agricultural product detection, in particular to a method for evaluating whether meat products contain antibiotics. Background technique [0002] Antibiotics have played an irreplaceable role in the development of modern aquaculture, but at the same time, they have brought drug residues to animal foods, posed a serious threat to food safety and human health, and attracted international attention and attention. Current studies have found that long-term intake of residual doses of antimicrobials can have adverse effects on intestinal flora, such as destroying the colonization resistance of intestinal flora, inducing and promoting the growth of drug-resistant bacteria; destroying the barrier function of intestinal flora, leading to potential Overgrowth of pathogenic bacteria; changes in the metabolic activity of intestinal flora; changes in the number and relative proportion of intestinal bacteria. [0003] my countr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/18
CPCC12Q1/18
Inventor 崔小燕魏泓刘建华王珊珊王玲玲
Owner 深圳市百澳飞生物技术有限公司
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