A measurement system based on single-molecule fluorescence microscopy to obtain polymer deformation

Abstract

The invention relates to a measuring system for acquiring polymer deformation based on single molecular fluorescence microscope imaging. The measuring system is characterized by comprising an excited light source unit, an optical microscopic unit, a high-precision mechanical drawing unit and a single molecular fluorescence imaging unit, wherein the excited light source unit is used for enabling one or more than one excited light source to generate circular polarized light so as to excite fluorescent probes in all directions and orientations; the optical microscopic unit is used for enabling the circular polarized light emitted by the excited light source unit to converge on a to-be-measured sample with deformation, collecting a fluorescence signal generated by the to-be-measured sample and exporting the fluorescence signal at the same time; the high-precision mechanical drawing unit is used for fixedly arranging the to-be-measured sample, and carrying out tensile test on the to-be-measured sample to obtain stress strain data of the to-be-measured sample; the single molecular fluorescence imaging unit is used for carrying out single molecular microscope imaging in the deformation process of the to-be-measured sample. According to the measuring system disclosed by the invention, the chain response behavior of glassy polymers in the deformation process can be researched more finely by using a single molecule level experimental test means.

Description

technical field [0001] The invention relates to a measurement system for acquiring polymer deformation based on single-molecule fluorescence microscopic imaging, and relates to the field of fundamental research of polymer physics to explore the deformation mechanism of polymers and the roots of brittleness and toughness. Background technique [0002] In the field of materials, glassy polymers have been widely used because of their good mechanical properties (such as engineering plastics) in addition to their advantages such as light weight, insulation, and easy processing. Polymers are generally more ductile than other types of high-modulus solid materials, and can undergo large deformations under external forces without breaking. Numerous studies are now seeking to further enhance the modulus and toughness of polymers while maintaining the good processability of polymers, which requires a deeper understanding of the mechanism of deformation of glassy polymers under external...

AI Technical Summary

Problems solved by technology

Unfortunately, this method obtains the statistical information of the chain motion, which cannot be used to describe the response at the molecular chain level

Based on molecular chains, Wang et al proposed a phenomenological load-bearing strands model to explain the brittleness, toughness and yield behavior of polymers during deformation, but so far the model still needs more evidence support

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