Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for increasing yield of validamycin

A technology of Jinggangmycin and Jinggang variant, applied in the field of microorganisms, can solve the problems of low yield and large consumption of raw materials, and achieve the effects of increasing yield, low cost and facilitating absorption

Active Publication Date: 2017-05-24
牡丹江佰佳信生物科技有限公司
View PDF1 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the method for preparing Jinggangmycin in the prior art has the problems of low yield and large consumption of raw materials

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] This example specifically provides a method for preparing Jinggangmycin by fermenting Streptomyces hygroscopicus varietal Jinggang. Contains the following steps:

[0029] Step (1): Inoculate the Streptomyces hygroscopicus varietal Jinggang in the slant culture medium, cultivate until the spores mature, and prepare the seed liquid; the components of the culture medium are: corn flour 30g / L, soybean cake powder 22g / L, yeast powder 10g / L, NaCl 2g / L, KH 2 PO 4 0.8g / L, CaCO 3 0.2g / L, the balance is water, adjust the pH to 7.0; set aside;

[0030] Step (2): Inoculate the seed liquid in the fermentation medium to carry out fermentation culture to obtain a fermentation liquid; the components of the fermentation medium are: corn flour 100g / L, soybean cake powder 25g / L, yeast powder 5g / L L, NaCl 1g / L, KH 2 PO 4 1.5g / L, CaCO 3 0.5g / L, cellulase 0.003g / L, and the balance is water; adjust the pH to 7.0, the fermentation culture is shaking culture, and the rotating speed o...

Embodiment 2

[0034] This example specifically provides a method for preparing Jinggangmycin by fermenting Streptomyces hygroscopicus varietal Jinggang. Contains the following steps:

[0035] Step (1): Inoculate the Streptomyces hygroscopicus varietal Jinggang in the slant culture medium, cultivate until the spores mature, and prepare the seed liquid; the components of the culture medium are: corn flour 25g / L, soybean meal powder 20g / L, yeast powder 15g / L, NaCl 1g / L, KH 2 PO 4 0.5g / L, CaCO 3 0.1g / L, the balance is water, adjust the pH to 7.2; set aside;

[0036] Step (2): Inoculate the seed liquid in the fermentation medium for fermentation and culture to obtain a fermentation liquid; the components of the fermentation medium are: corn flour 90g / L, soybean cake powder 30g / L, yeast powder 8g / L L, NaCl 2g / L, KH 2 PO 4 3g / L, CaCO 3 0.6g / L, cellulase 0.001g / L, the balance is water; adjust pH to 7.2, described fermentation culture is vibration culture, and the rotating speed of vibrat...

Embodiment 3

[0040] This example specifically provides a method for preparing Jinggangmycin by fermenting Streptomyces hygroscopicus varietal Jinggang. Contains the following steps:

[0041] Step (1): inoculate the Streptomyces hygroscopicus varietal Jinggang in the slant culture medium, cultivate until the spores mature, and prepare the seed liquid; the components of the culture medium are: corn flour 28g / L, soybean meal powder 22g / L, yeast powder 13g / L, NaCl 2g / L, KH 2 PO 4 0.6g / L, CaCO 3 0.15g / L, the balance is water, adjust the pH to 6.8; set aside;

[0042] Step (2): Inoculate the seed liquid in a fermentation medium for fermentation and culture to obtain a fermentation liquid; the components of the fermentation medium are: corn flour 95g / L, soybean cake powder 28g / L, yeast powder 7g / L L, NaCl 2g / L, KH 2 PO 4 2g / L, CaCO 3 0.5g / L, cellulase 0.002g / L, the balance is water; adjust pH to 7.5, described fermentation culture is vibration culture, and the rotating speed of vibrati...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to the field of microorganisms and particularly provides a method for increasing the yield of validamycin. The validamycin is a plant-derived fungicide, belongs to water-soluble antibiotics-glucoside compounds generated by streptomyces hygroscopicus var. jinggangensis, and contains six components of A, B, C, D, E and F in total; a main active substance of the validamycin is validamycin A. According to the method disclosed by the invention, by further optimizing specific components of a streptomyces hygroscopicus var. jinggangensis fermentation medium and other culture conditions, the yield of the validamycin is increased; the yield of the validamycin disclosed by the invention can reach as high as 27000[mu]g / mL; in addition, the method has the advantages of simple scheme, low cost and suitability for industrial large-scale production.

Description

technical field [0001] The invention relates to the field of microorganisms, and specifically provides a method for preparing Jinggangmycin by fermenting Streptomyces hygroscopicus Jinggang variety. Background technique [0002] Jingangmycin is a plant-derived fungicide. It is a water-soluble antibiotic-glucoside compound produced by Streptomyces hygroscopicus. It has six components: A, B, C, D, E, and F. , the main active substance is Jinggangmycin A, and the content of component A in general products is used to indicate the specifications and quality of the product. Jinggangmycin is an antibiotic produced by actinomycetes. It has strong systemic properties and is easily absorbed by bacterial cells and rapidly transmitted in it, interfering with and inhibiting the growth and development of bacterial cells. Jinggangmycin is mainly used for wheat sheath blight, and it can also be used for rice false smut, corn spot disease, vegetables, cotton, beans and other crop diseases. ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/46C12R1/55
Inventor 周贤龙石怀月刘静
Owner 牡丹江佰佳信生物科技有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com