59 results about "Validamycin A" patented technology

The invention discloses a method for preparing acarbose through microbial fermentation. The method comprises the following steps: inoculating acarbose producer (CCTCC NO:M 209022) in fermentation medium which is suitable for the strain and contains carbon source, nitrogen source and inorganic salt, fermenting and culturing at 20-32 DEG C for 96-192 hours, obtaining fermentation liquor after fermentation, extracting to obtain acarbose. The method is characterized in that fermenting and culturing are performed for 0-48h and validamycin A solution is added to ensure that the one litre of fermentation medium contains 0.04-0.4g of validamycin A. By supplementing validamycin A in the fermentation process of acarbose, the fermentation level of acarbose can be increased.

A chemically-biological synergistic bactericide for preventing and treating the Rhizoctonia diseases and fungal diseases of agricultural crops contains jinggangmycin A (validamycin A), kresoxim-methyl, filler, solvent, water and assistant.

The invention relates to a pesticide composition and application thereof, and particularly relates to a sterilization composition containing amino-oligosaccharin and validamycin A as main active ingredients and application of the pesticide composition. The active ingredients of the sterilization composition are amino-oligosaccharin and validamycin with the weight ratio of 1:1 to 1:50, preferably, 1:2 to 1:10. The sterilization composition containing the amino-oligosaccharin and the validamycin, provided by the invention has an excellent control effect, which is better than that of a single-agent variety, for the rice sheath blight disease through actual field trials, and has no pesticide damage through field observation; the field growth vigour is better than that of a contract single-agent pesticide.

The invention relates to a fermentation production method of validamycin A in the technical field of biological engineering. The method comprises the following steps: using spore suspension to perform activation of strains and plate culture and prepare spore activation fluid; then inoculating the spore activation fluid in strain medium to perform preliminary culture and obtain strain culture fluid; and finally inoculating the strain culture fluid in fermentation medium to perform fermentation culture and realize the fermentation production of validamycin A. By using the method, the yield of validamycin A can be further increased; and under the premise of not increasing energy consumption, the method can shorten fermentation period, increase equipment utilization rate, obtain higher yield of validamycin A and reduce production cost, thus the method has application value in the industrial production.

This invention discloses engineering bacterium containing beta-glucosidase, Escherichia coli (CGMCC No.1626), and its application in preparing validoxylamine A compounds. The engineering bacterium can convert validamycin A into validoxylamine A, and has such advantages as high conversion efficiency, low cost, mild reaction conditions, high product purity, easy separation and purification, and little chemical pollution.

The present invention relates to a compound germicide which can be used for effectively controlling crop diseases of rice sheath blight disease and green smut of rice, etc. Said compound germicide is made up by using copper sulfate and jinggangmeisu (validamycin A) as effective components according to the mixing ratio of 1:12-12:1 through a certain preparation process.

The invention relates to a pesticide composition, in particular to a pesticide composition containing tebuconazole and validamycin A in effective dose for synergism, wherein the weight ratio of the tebuconazole to the validamycin A is preferably 10:1-1:10, more preferably 10:1-1:6, further preferably 1:1-1:6 and optimally preferably 1:3. The pesticide composition is particularly suitable for controlling false smut of rice.

The invention discloses a complex biological fungicide for preventing and treating wheat scab. The complex biological fungicide comprises validamycin A and JS399-19, wherein the weight ratio of the validamycin A to the JS399-19 ranges from 1:20-20:1. The invention further discloses application of the complex biological fungicide in preventing and treating wheat scab. The validamycin A and the JS399-19 in the complex biological fungicide are fungicides with different functional mechanisms and do not have cross-resistance with carbendazim and triadimefon, wherein carbendazim and triadimefon are leading medicine for preventing and treating wheat scab in the past. The complex biological fungicide has the advantages of being wide in bacteriocidal spectrum, high in absorption, good in fast-acting property, long in preserving effect period, environmentally friendly, low in cost and easy to process.

The invention provides a sterilizing composition containing cyproconazole and validamycin A and a preparation method thereof. Cyproconazole and validamycin A are used as main components, and deionized water and an auxiliary agent are assisted to form a suspending agent or the main components, the auxiliary agent and a carrier form wettable powder; and the compounding of the cyproconazole and the validamycin A can generate higher synergistic effect, expand the disease preventing and treating range, delay the generation of drug resistance, obviously improve the pesticide effect, lightens the burden on peasants and guarantees the safe production of agriculture and ecological environment. The invention is applied to the field of chemical protection of agricultural plants and has excellent andefficient protecting and treating effects on fruit trees, vegetables, wheat, rice, and the like with pathogenic bacterial damages.

The invention relates to a pesticide combination, in particular to a pesticide combination containing effective dose of synergic action propiconazole and validamycin A, wherein the weight ratio of the propiconazole to the validamycin A is preferably 10:1 to 1:10, more preferably 10:1 to 1:6, further preferably 1:1 to 1:6, and most preferably 1:2 to 1:6. The pesticide combination is particularly suitable for preventing and treating the holing illness and the ustilaginoidea virens of the paddy rice.

The invention discloses streptomyces hygroscopicus KN-055 for producing validamycin.The streptomyces hygroscopicus KN-055 is deposited in the China Center for Type Culture Collection with the deposit number of CCTCC NO: M 2015605.By the streptomyces hygroscopicus KN-055, the content of validamycin A in validamycin fermentation liquor can be increased, and the content of validamycin B is reduced.Compared with an original strain, the streptomyces hygroscopicus KN-055 has the features that the content of the validamycin A is increased by 26.8%, B / A is reduced by 18.2%, the yield of the validamycin can be stably above 25,000 micro g / mL, the genetic stability of the KN-055 strain is good, and the validamycin producing level of the fourth-generation streptomyces hygroscopicus KN-055 is equal to that of the first-generation streptomyces hygroscopicus KN-055.

The invention discloses a bactericidal composition containing penconazole and validamycin A and a preparation method thereof, wherein penconazole and validamycin A are used as main components and then assisted with deionized water and an auxiliary agent to form a suspending agent, or form a wettable powder together with the auxiliary agent and a carrier. The penconazole and the validamycin A are compounded, and a higher synergistic action can be generated, thereby the disease control range is widened, the generation of drug resistance is delayed, the drug effect is markedly improved, the burden on peasants is lightened, and agricultural safe production and ecological environments are also ensured. The bactericidal composition is applied to the field of agricultural plant chemical protection and has excellent and highly efficient protecting and treating actions for the pathogen harms of fruit trees, vegetables, wheat, rice and the like.

The invention discloses a bactericidal composition containing epoxiconazole and validamycin A and a preparation method thereof, wherein epoxiconazole and validamycin A are used as main components and then assisted with an auxiliary agent and deionized water to form a suspending agent, or form a wettable powder together with the auxiliary agent and a carrier. The epoxiconazole and the validamycin A are compounded, and a higher synergistic action can be generated, thereby the disease control range is enlarged, the generation of drug resistance is delayed, the drug effect is markedly improved, the burden on peasants is lightened, and agricultural safe production and ecological environments are also ensured. The bactericidal composition is applied to the field of agricultural plant chemical protection and has excellent and highly efficient protecting and treating actions for the pathogen harms of fruit trees, vegetables, wheat, rice and the like.

The invention discloses a one-step validamycin extraction method from macroporous weak acid absorption resin. The method comprises the following steps: isoelectrofocusing a validamycin fermentation broth, filtering before cooling, adjusting a pH value of filtrate to 7 to 9, cooling to a room temperature, absorbing with the macroporous weak acid absorption resin, and washing with deionized water to be neutral; then, desorbing with 1.0 to 2.0mol / L of ammonia and concentrating collected eluate by decompression until the concentration of validamycin is 10 Baume so as to obtain a validamycin aqueous solution; and absorbing with activated carbine, discoloring and concentrating to 15 Baume to 20 Baume and spraying and drying in vacuum at 40 DEG C to 70 DEG C to obtain validamycin powder which contains about 65 percent of validamycin A. The method has the advantages of short technological flows, great absorption, short desorption time, high extraction yield, little wastewater and waste gas emission and low cost.

The invention discloses a method for extracting validamycin A from validamycin fermentation liquor and refining the validamycin A. The method comprises the following steps: A. the validamycin fermentation liquor is directly handled by using a sawtooth ultrafiltration membrane system, the automatic control is adopted for the membrane system, and the output ultrafiltrate can directly enter in an ion exchange system after the validamycin fermentation liquor enters in the membrane system; B. cation exchange resin and anion exchange resin are selected and combined, and the foreign ions in the validamycin filtrate are removed; C. macroporous cation exchange resin is selected for absorbing the validamycin A, and the resin has good selectivity, large adsorbing capacity of the validamycin A, high mechanical strength and anti-pollution capability and long service life and is not easily broken; and D. due to the adoption of the PH gradient adsorption process, the PH value of the validamycin filtrate entering in the adsorption column is improved by using the ion exchange reaction balancing principle. The invention not only improves the adsorption efficiency and the saturated extent of adsorption of the adsorption resins, but also reduces the consumption of acid, alkali and water which are required by the anion resin (SX-14) and the activated resin and also improves the production efficiency.

The invention discloses a qualitative and quantitative detection method for each component of validamycin. The qualitative and quantitative detection method comprises the steps of A, identification test; and B, determination on the mass fraction of each component of validamycin, wherein the step particularly comprises the steps of 1) reagent and solution preparation; 2) instrument preparation; 3) sample treatment; 4) chromatograph operating conditions; 5) sampling program list compilation; 6) determination: after the base line of an instrument is stabilized, clicking a start-up button in a chemical workstation, automatically extracting standard sample solution by using the automatic sampler of a chromatographic instrument, quantifying in a quantifying ring, bringing the quantified standard sample solution into a chromatographic column by a mobile phase to separate, and sequentially conveying each component of validamycin after being separated into a detector flow cell; and 7) data analysis: drawing a standard curve, recording the peak area of each component, and quantifying each component of validamycin by combining with the standard curve. The method is high in sensitivity and good in selectivity; validamycin A and the related components can achieve base-line separation; and accurate qualification and quantification can be performed on each component in the related products of validamycin.

The invention discloses a biological compound agent for preventing and controlling fungus diseases, for example, rice sheath blight disease, in particular to a difenoconazole and validamycin A wettable powder. 20 to 80 mg of difenoconazole, 40 to 160 mg of validamycin, wetting agent, dispersant and filling agent are contained in the powder per gram. The difenoconazole is an efficient systemic fungicide, and the validamycin is biological antibiotics, therefore, the difenoconazole and validamycin A wettable powder has the dual effects of bacteria resistance and sterilization. The control effect of the difenoconazole and validamycin A wettable powder to the rice sheath blight disease reaches 76.2 to 91.7 percent through field test by the relevant unit, and compared with the single validamycin, the difenoconazole and validamycin A wettable powder has the advantages that the control effect against the rice sheath blight disease is increased by 12.3 to 20.5 percent, and the effect of increase in yield is improved by 13.6 percent.

The invention discloses a biological pesticidal and sterilizing composition containing validamycin A and avermectin and a production method thereof. The composition comprises the following effective components: 2-20% of validamycin A and 1-5% of avermectin. The production method is realized by the following steps: adding the raw materials into an enamel stirred tank, evenly mixing, and pulverizingby air flows or milling to obtain the biological pesticidal and sterilizing composition containing validamycin A and avermectin, which is in the state of the wettable powder or suspending agent. Since the validamycin A and the avermectin are compounded for use, the composition has the characteristics of low toxicity, low residue, wide pesticidal range, favorable lasting effect, low cost and especially biological prevention and control capability without environmental disruption, and thereby being an ideal biological pesticide for preventing and controlling various plant diseases and insect pests of rice.

The invention discloses an azoxystrobin and validamycin A composite bactericide, and belongs to the technical field of pesticides. The composite bactericide comprises 0.1-50% of azoxystrobin, and 0.5-60% of validamycin A. The azoxystrobin and validamycin A composite bactericide is reasonably composited, has a significant synergic effect, and good effects for preventing rice sheath blight disease, rice false smut and wheat sharp eyespot. Proved by a filed test, a control rate can be up to 97% above. A persistence property is strong; after 14 days of an application for preventing the rice sheath blight disease and the wheat sharp eyespot, the control rate can be up to 92% above. Production cost is low and reduced by 50% above, compared with a single use of the azoxystrobin.

The invention discloses a preparation method of high-purity validamycin A. The preparation method comprises the steps that firstly validoxylamine A is adsorbed on cationic resin, colibacteriophage serves as a glycosyl transferase catalyst, cellobiose is added into water to enable the final concentration of the cellobiose to be 0.05-1.5% g / mL, and the reaction is conducted for 12-48 h under the conditions that the reaction temperature is 25-35 DEG C, and the pH is 5.0-7.0; after the reaction is finished, filtering is conducted, a filter cake is taken to be eluted by the use of a 0.2-1.2 mol / L ammonia-water solution, a validamycin A component is traced, detected and collected through HPLC, spray drying or freeze drying is conducted after concentration to prepare and obtain the validamycin A, and the mass ratio of the validoxylamine A to the cellobiose is 1:1.5-2.5. According to the preparation method of the high-purity validamycin A, the cation exchange resin is used for adsorbing the validoxylamine A and the validamycin A, not only can the substrate be avoided inhibiting enzymes, but also the products can be avoided inhibiting the enzymes, and the prepared validamycin A is high in product purity.

The invention provides a wettable powder containing Jinggangmycin and difenoconazole and a preparation method thereof. The present invention is characterized in that the content of each component of the suspending agent of Jinggangmycin and difenoconazole is (mass fraction): Jinggangmycin 0.1-70%, difenoconazole 0.1-70%, dispersant 1 -5%, wetting agent 1-5%, penetrating agent 0.5-2%, thickener 0.2-1%, filler balance. The invention is mainly used for preventing and controlling various fungal venereal diseases mainly including rice sheath blight.

The invention relates to a prevention and control agent for banded sclerotial blight of crops. The active ingredients of the prevention and control agent for banded sclerotial blight of crops are validamycin and glucosene, wherein the validamycin is validamycin A or a mixture of the validamycin A and isomers B, C, D, E and F of the validamycin A. The active ingredients comprise the following components in parts by weight: 0.1-60.0 parts of validamycin and 0.1-25.0 parts of glucosene. According to a synergistic effect of the bactericidal activity of the validamycin and the induced resistance of the glucosene, the occurrence and development of the banded sclerotial blight of crops are prevented and controlled, the agent has the prevention and treatment effects, and obvious prevention and treatment effects can be achieved when the agent is taken before or after pathogenesis.

The invention discloses a recombined engineering bacterium for producing UDPG (uridine diphosphate glucose) and application thereof to preparation of validamycin A. The recombined engineering bacterium is formed by transferring the ValG gene in streptomyces hygroscopicus and the GalU gene and the Pgm gene in escherichia coli JM109 into a host bacterium, wherein the ValG gene has a nucleotide sequence as shown in SEQ ID NO:1, the GalU gene has a nucleotide sequence as shown in SEQ ID NO:2, and the Pgm gene has a nucleotide sequence as shown in SEQ ID NO:3. The recombined engineering bacterium can be used for transforming validoxylamine A into validamycin A under the whole cell catalysis, has a high transformation speed in the same time and can achieve a transformation rate of 75%.

The invention relates to the field of microorganisms and particularly provides a method for increasing the yield of validamycin. The validamycin is a plant-derived fungicide, belongs to water-soluble antibiotics-glucoside compounds generated by streptomyces hygroscopicus var. jinggangensis, and contains six components of A, B, C, D, E and F in total; a main active substance of the validamycin is validamycin A. According to the method disclosed by the invention, by further optimizing specific components of a streptomyces hygroscopicus var. jinggangensis fermentation medium and other culture conditions, the yield of the validamycin is increased; the yield of the validamycin disclosed by the invention can reach as high as 27000[mu]g / mL; in addition, the method has the advantages of simple scheme, low cost and suitability for industrial large-scale production.

The invention discloses a preparation method of raw validamycin powder. The preparation method comprises the steps of centrifuging a validamycin fermentation solution at a high speed to remove mycelium; filtering the centrifugal liquid by using a ceramic micro-filtration membrane with the aperture of 1-10mu m; then, adding nanosilicon dioxide, nano aluminum oxide, thermoplastic acrylic resin and a surfactant with the HLB (Hydrophile-Lipophile Balance) value of 15-20 into the filtrate according to a certain weight, stirring for 10-30min, and regulating the pH value of the filtrate to 7.5-8.5; filtering by a hollow fiber ultrafiltration membrane of which the molecular cut off is 10000-20000 Dalton, collecting the filtrate, carrying out activated carbon decoloration, concentrating and drying to obtain the raw validamycin powder. The preparation method is capable of preparing the raw validamycin powder with high validamycin A and validamycin B contents, high in ultrafiltration efficiency and long in service life of the ultrafiltration membrane. In addition, the preparation method has the characteristics of simple steps, short production period, high efficiency and the like.

The invention relates to a bactericide containing benzene kresoxim-methyl and validamycin A and used for the prevention and treatment of rice sheath blight disease and green smut, belonging to the technical field of agricultural chemicals. The bactericide containing benzene kresoxim-methyl and validamycin A contains the effective ingredients of benzene kresoxim-methyl and validamycin A, wherein the weight ratio of the benzene kresoxim-methyl and the validamycin A is 1:50-5:1. According to actual tests, the bactericide has outstanding prevention and treatment effect against rice sheath blight disease and green smut. Compared with conventional bactericides, the bactericide of the invention has the following advantages of: 1, obvious synergized action against rice sheath blight disease and green smut compared with single bactericide and being capable of remarkably enhancing the prevention and treatment effect; 2, being capable of replacing a part of medium / high-toxicity bactericides, lowering dose, reducing environmental pollution and residual quantity on agricultural products; 3, being capable of delay the resistance of rice sheath blight disease and green smut to single bactericide; and 4, long effecting period and lowered cost compared with single bactericide.

The invention discloses a streptomyces corchorusii strain KN-1625 producing validamycin. The strain is deposited in the China Center for Type Culture Collection and has an accession number of CCTCC NO:M2015710. The strain can increase the content of validamycin A in a validamycin fermentation liquid, and can reduce the content of validamycin B. Compared with original strains, the content of the validamycin A when the strain is adopted is increased by 47%, and a ratio (B / A) of the content of the validamycin A to the content of the validamycin B is reduced by 37.56%. The validamycin yield is maintained to be higher than 30000 [mu]g / mL. The KN-1625 strain is good in hereditary stability. A validamycin level of the fourth generation is equivalent to that of the first generation.

A method which adopts jinggangmycin non-foliage spray to prevent and control sheath blight of rice and wheat belongs to the method which prevents rice and wheat diseases, and solves problems existing in other technologies. The method of the invention is as follows: when a field is ploughed, 200g of 20% of jinggangmycin is added with 15kg of water per mu, dissolved fully and stirred evenly, and 25kg of grass meal, sawdust and chaff, or 50kg of sandy soil is mixed with a fertilizer to obtain a mixture, and then the mixture is scattered on the surface of the field, and ploughed in the soil. For 3 years, the method is applied as a demonstration by 6000 peasants in 100 villages of 13 towns, and achieves the preventative effect on the sheath blight of the wheat and the rice.

The invention relates to a method for producing wettable powder through the compound of validamycin A and propiconazole. The method comprises following steps of (1) adding 2-5 times volume of absolute ethyl alcohol or acetone into original propiconazole based on the volume of the original propiconazole, and dissolving; (2) adding white carbon black into the propiconazole ethyl alcohol solution or propiconazole acetone solution, fully mixing so as to form pulp; (3) vacuum drying, and smashing so as to obtain propiconazole mother powder; (4) fully mixing with wetting dispersant and kaolin; (5) smashing through airflow; (6) adding original medicine powder containing 60%-70% of validamycin A, and mixing to obtain a mixture; (7) performing multipoint sampling and detecting on the mixture, packaging and putting in storage after all indexes are qualified through detection. The method is reasonable and is convenient to operate, the homogeneity of medicines of the prepared wettable powder product is good, the suspensibility of the wettable powder is high and is greater than or equal to 85%.