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One-step validamycin extraction method from macroporous weak acid absorption resin

A technology of Jinggangmycin and adsorption resin, which is applied to the field of one-step extraction of Jinggangmycin from macroporous weak acid adsorption resin, can solve the problems of large waste water discharge, long process flow, long desorption time, etc. The effect of short, short desorption times

Inactive Publication Date: 2009-09-09
HUNAN NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are problems such as long process flow, low adsorption capacity, long desorption time, low yield, large wastewater discharge, and high cost.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] (1) Utilize the bacterial classification and method used in the prior art to carry out the fermentation of Jinggangmycin:

[0017] Strains such as: Streptomyces hygroscopicus var. Jinggangensis. Composition of fermentation medium: rice flour 7.0%, soybean cake flour 2.0%, peanut cake flour 1.0%, yeast powder 1.0%, MgSO 4 0.05%, NaCl 0.5%, KH 2 PO 4 0.05%, fermented in a stirred fermenter at a fermentation temperature of 38-42°C, an aeration rate of 0.7-1.3vvm, and a fermentation time of 38-44h to obtain Jinggangmycin fermentation broth.

[0018] (2) Take 10L of fermentation broth and wait for electricity to filter it while it is hot, adjust the pH of the filtrate to 7.5 with sodium hydroxide, and the filtrate is absorbed by D151 macroporous weak acid adsorption resin at a flow rate of 1.0m 3 / m 3 Resin h, until the resin is saturated (point plate tracking detection); wash the resin with deionized water twice the volume of the resin, and the washing speed is 3.0m 3...

Embodiment 2

[0022] The fermentation broth preparation method of Jinggangmycin is the same as in Example 1.

[0023] Take 10L of fermented fermentation broth that has been fermented and wait for electricity to filter while it is hot. Use sodium hydroxide to adjust the pH of the filtrate to 8, and the filtrate is adsorbed by D151 macroporous weak acid adsorption resin at a flow rate of 1.5m 3 / m 3 Resin · h, until the resin adsorption saturation (dot plate tracking detection). Then wash the resin with deionized water twice the volume of the resin, and the washing speed is 1.5m 3 / m 3 Resin h. After washing, use 1.5mol / L ammonia solution as eluent, and elute to the end point (spot plate tracking detection) according to the conventional method, and the elution speed is 1.0m 3 / m 3 Resin h. The eluate obtained was collected and concentrated under reduced pressure until the concentration of Jinggangmycin was 10 Baume. The concentrated solution obtained was decolorized with activated car...

Embodiment 3

[0025] The fermentation of Jinggangmycin is the same as in Example 1.

[0026] Take 10L of the fermented broth that has been fermented and wait for electricity while it is still hot. Use sodium hydroxide to adjust the pH of the filtrate to 8.5, and the filtrate is adsorbed by D151 macroporous weak acid adsorption resin at a flow rate of 1.5m 3 / m 3 Resin · h, until the resin adsorption saturation (dot plate tracking detection). Then wash with 2 times the volume of resin in deionized water, the washing speed is 2.0m 3 / m 3 Resin h. After washing, use 1.5mol / L ammonia solution as eluent, and elute to the end point (spot plate tracking detection) according to the conventional method, and the elution speed is 1.0m 3 / m 3 Resin h. The eluate obtained was collected and concentrated under reduced pressure until the concentration of Jinggangmycin was 10 Baume. The concentrated solution is decolorized with activated carbon, and after the decolorization is completed, it is furth...

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PUM

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Abstract

The invention discloses a one-step validamycin extraction method from macroporous weak acid absorption resin. The method comprises the following steps: isoelectrofocusing a validamycin fermentation broth, filtering before cooling, adjusting a pH value of filtrate to 7 to 9, cooling to a room temperature, absorbing with the macroporous weak acid absorption resin, and washing with deionized water to be neutral; then, desorbing with 1.0 to 2.0mol / L of ammonia and concentrating collected eluate by decompression until the concentration of validamycin is 10 Baume so as to obtain a validamycin aqueous solution; and absorbing with activated carbine, discoloring and concentrating to 15 Baume to 20 Baume and spraying and drying in vacuum at 40 DEG C to 70 DEG C to obtain validamycin powder which contains about 65 percent of validamycin A. The method has the advantages of short technological flows, great absorption, short desorption time, high extraction yield, little wastewater and waste gas emission and low cost.

Description

technical field [0001] The invention relates to a method for extracting Jinggangmycin, in particular to a one-step method for extracting Jinggangmycin with a macroporous weak acid adsorption resin. Background technique [0002] Jinggangmycin is an environmentally friendly pesticide with "high control efficiency, no harm, and no pollution", and is very popular among users at home and abroad. The existing extraction method of Jinggangmycin is the method of microbial enzymolysis, which is to filter the fermented liquid after isoelectricity, concentrate the filtrate to obtain an aqueous solution, and dry the concentrated solution to obtain a powder; thus, the content of the aqueous solution is about 10%. , the powder content is about 20%, but in the Jinggangmycin fermentation broth, in addition to containing about 3% of Jinggangmycin components, it also contains a lot of impurities: biological cells and debris, residual sugar, miscellaneous proteins, inorganic salts, pigment et...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/46C07H15/22C07H1/08C12R1/55
Inventor 钟世华徐满才齐风佩范明亮
Owner HUNAN NORMAL UNIVERSITY
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