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Streptomyces hygroscopicus for producing validamycin and application of streptomyces hygroscopicus

A technology of Streptomyces hygroscopicus, Jinggangmycin, applied in bacteria, microorganism-based methods, microorganisms, etc., can solve problems such as poor product quality

Active Publication Date: 2016-07-13
武汉科诺生物科技股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the Jinggangmycin impurity content produced at present is higher, especially the content of Jinggangmycin B is higher, and the product quality is generally poor

Method used

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  • Streptomyces hygroscopicus for producing validamycin and application of streptomyces hygroscopicus

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0009] Mutagenesis and screening of embodiment 1 bacterial strain

[0010] 1. Using ion beam mutagenesis to treat the bacterial suspension to obtain mutant strains

[0011] Take an appropriate amount of slant cells or spores produced by Jinggangmycin, and place them in sterile water containing glass beads, so that the concentration of cells or spores is at 10 6 ~10 8 per ml, shake well to disperse. Draw 0.2 ml of the suspension into a petri dish with a diameter of 9 cm, spread it evenly with a stick, and blow it in an aseptic wind for 0.5 hours in an ultra-clean bench until it dries into plaques. The nitrogen ion energy is 7Kev, and the dose is 5×10 13 , 10×10 13 , 15×10 13 , 20×10 13 , 25×10 13 Mutagenesis was carried out at five levels, each experiment was treated accordingly, and the shake flask was the same as the starting strain, and the mutagenic dose was determined to be 10×10 through repeated experiments. 13 When N+ / CM, the positive mutation rate of the strain ...

Embodiment 2

[0026] Embodiment 2 Utilizes the experimental research of bacterial strain production Jinggangmycin

[0027] 1) Strain activation

[0028] The preserved Streptomyces hygroscopicus KN-055 strain was activated on Gaoshi No. 1 slant medium, cultured at 28°C for 7 days, and the bacterial lawn was washed with sterile water to obtain a seed suspension;

[0029] 2) Secondary seed cultivation

[0030] Put the seed suspension obtained in step 1) into a seed fermenter, 200rpm, and cultivate at 37°C for 22-24hr. The composition of the seed fermentation medium is: 5% corn flour, 2.4% starch, 0.5% soybean meal, 0.05% NaCl, CaCO 3 0.05%, KH 2 PO 4 0.05%, the balance is water, and the content of each component is according to the mass volume ratio;

[0031] 3) Fermentation culture

[0032] Transfer the fermented seed liquid obtained after step 2) into a large fermenter for cultivation, set the fermentation parameters as follows: aeration ratio 1:1, stirring speed 420rpm, fermentation te...

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Abstract

The invention discloses streptomyces hygroscopicus KN-055 for producing validamycin.The streptomyces hygroscopicus KN-055 is deposited in the China Center for Type Culture Collection with the deposit number of CCTCC NO: M 2015605.By the streptomyces hygroscopicus KN-055, the content of validamycin A in validamycin fermentation liquor can be increased, and the content of validamycin B is reduced.Compared with an original strain, the streptomyces hygroscopicus KN-055 has the features that the content of the validamycin A is increased by 26.8%, B / A is reduced by 18.2%, the yield of the validamycin can be stably above 25,000 micro g / mL, the genetic stability of the KN-055 strain is good, and the validamycin producing level of the fourth-generation streptomyces hygroscopicus KN-055 is equal to that of the first-generation streptomyces hygroscopicus KN-055.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a Jinggangmycin-producing Streptomyces hygroscopicus, and also relates to the application of the strain. Background technique [0002] Jinggangmycin is an agricultural antibiotic with strong systemic properties, easily absorbed by bacterial cells and rapidly transmitted in the body, interfering with and inhibiting the growth and development of bacterial cells. Its main purpose is to prevent and control rice sheath blight and wheat Sheath blight is an environmentally friendly pesticide with "high control efficiency, no harm, and no pollution". [0003] Jinggangmycin is composed of A, B, C, D, E, F and other components, but only A component has the strongest control effect on rice sheath blight. The high content of Jinggangmycin A can not only be used as a pesticide, but also It can be used as an intermediate in the production of medicine, and its application prospect is b...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12P19/46C12R1/55
CPCC12P19/46C12N1/205C12R2001/55
Inventor 李青夏建荣程治国邹维唐维坤周莉万俊潘瑜王梦婕刘华梅
Owner 武汉科诺生物科技股份有限公司
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