Qualitative and quantitative detection method for each component of validamycin

Abstract

The invention discloses a qualitative and quantitative detection method for each component of validamycin. The qualitative and quantitative detection method comprises the steps of A, identification test; and B, determination on the mass fraction of each component of validamycin, wherein the step particularly comprises the steps of 1) reagent and solution preparation; 2) instrument preparation; 3) sample treatment; 4) chromatograph operating conditions; 5) sampling program list compilation; 6) determination: after the base line of an instrument is stabilized, clicking a start-up button in a chemical workstation, automatically extracting standard sample solution by using the automatic sampler of a chromatographic instrument, quantifying in a quantifying ring, bringing the quantified standard sample solution into a chromatographic column by a mobile phase to separate, and sequentially conveying each component of validamycin after being separated into a detector flow cell; and 7) data analysis: drawing a standard curve, recording the peak area of each component, and quantifying each component of validamycin by combining with the standard curve. The method is high in sensitivity and good in selectivity; validamycin A and the related components can achieve base-line separation; and accurate qualification and quantification can be performed on each component in the related products of validamycin.

Description

technical field [0001] The present invention relates to the technical field of quantitative detection of Jinggangmycin in related products of Jinggangmycin (Validamycin), more specifically to a qualitative and quantitative detection method of Jinggangmycin A and related components in Jinggangmycin products. Available Qualitative and quantitative detection of each component of Jinggangmycin-related products and provide a basis for the production of high-quality Jinggangmycin products. Background technique [0002] Jinggangmycin (Validamycin) is a water-soluble antibiotic glucoside compound produced by Streptococcus hygroscopicus Jinggang variety, molecular formula C 20 h 35 o 13 N, molecular weight 497.51, low toxicity, pure white amorphous powder, soluble in water, dimethylformamide, slightly soluble in ethanol, insoluble in acetone, benzene, ethyl acetate, and other organic solvents, strong hygroscopicity, in Stable in PH3-9 aqueous solution at room temperature. Jinggan...

AI Technical Summary

Problems solved by technology

At present, domestic quantitative analysis methods for Jinggangmycin include ion-exchange colorimetry and thin-layer fluorescence method (Xu Hui, Determination of Jinggangmycin Aqua by TLC-Fluorescence Method, Journal of Shanghai Agricultural University 1998, 16 (2): 148 -151.), liquid chromatography (GB / T9553-93), the first two can only measure the total content of Jinggangmycin, and cannot separate and quantify the components; the liquid chromatography used in GB / T9553-93 is also Can only separate and quantify Jinggangmycin A

There are also relevant reports in Japan on the detection of VA, VM-B, VM-C, VM-D, VM-E, VM-F, and VxA-A in Jinggangmycin products, and the detection method uses 100% 20mmol / L phosphoric acid Buffer salt is used as the mobile phase. Although this method can basically separate the different components in Jinggangmycin products, the separation effect of each component is not ideal.

Its shortcomings are as follows: 1. The system pressure is as high as 200bar or more. With the increase of the detection amount, the pressure will continue to rise. The high concentration and high proportion of buffer salt can easily damage the structure of the chromatographic column, resulting in an extremely short life of the chromatographic column; 2. 1. The analysis time is as long as 1 hour, which is not realistic enough for mass production inspection; 3. Due to the long time, the peak shape becomes poor, and with the extension of time, it is easy to produce baseline drift, which will cause inaccurate quantification; 4. Response value Small, the same injection concentration and injection volume, its peak height and peak area response is basically one-third of the present invention

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