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A streptomyces corchorusii strain producing validamycin

A technology of Streptomyces jute and Jinggangmycin, applied in the direction of bacteria, microbe-based methods, microbes, etc., can solve problems such as poor product quality

Active Publication Date: 2016-05-04
WUHAN KERNEL BIO-TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the Jinggangmycin impurity content produced by the microbial fermentation method is relatively high, especially the content of Jinggangmycin B is relatively high, and the product quality is generally poor.

Method used

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  • A streptomyces corchorusii strain producing validamycin
  • A streptomyces corchorusii strain producing validamycin

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0008] Mutagenesis and screening of embodiment 1 bacterial strain

[0009] 1. Treat the bacterial suspension with 60CO-γ rays to obtain mutant strains

[0010] Take an appropriate amount of slanted cells or spores of Streptomyces jute KN-11, place them in sterile water containing glass beads, make the concentration of cells or spores 106-108 / ml, and shake well to disperse. Due to the strong penetrating ability of gamma shooting rays, the lethal effect on cells is stronger than that of general chemical mutagens. The mutagenic dose should be controlled at a lethal rate of 90-99%. Taking KN-16 as the starting strain, irradiate its spore suspension with different doses of 60CO-γ rays. The doses are 300Gy, 500Gy, 700Gy, 1000Gy, 1300Gy, At 1500Gy, the mortality rates were 75.7%, 85.3%, 89.1%, 95.0%, 98.2%, and 99.10%, respectively. The death rate of spores increased very quickly within 300Gy, and the change of death rate of spores under 500-1500Gy was relatively slow.

[0011] Th...

Embodiment 2

[0025] Embodiment 2 Utilizes the experimental research of bacterial strain production Jinggangmycin

[0026] 1) Strain activation

[0027] The preserved KN-1625 strain was activated on Gaoshi No. 1 slant medium, cultured at 28°C for 7 days, and the bacterial lawn was washed with sterile water to obtain a seed suspension;

[0028] 2) Secondary seed cultivation

[0029] The seed bacteria suspension obtained in step 1) is connected to a seed fermenter, 200 rpm, and cultivated at 40° C. for 16-18 hr. The composition of the seed fermentation medium is: rice flour 4.66%, corn flour 2.52%, soybean meal 1.033%, NaCl 0.03%, CaCO30.05%, KH2PO40.03%, the balance is water, and the content of each component is according to the mass volume ratio;

[0030] 3) Fermentation culture

[0031] Transfer the seed fermentation liquid obtained after step 2) into a large fermenter for cultivation, set the fermentation parameters as follows: aeration ratio 1:1, stirring speed 420rpm, fermentation te...

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Abstract

The invention discloses a streptomyces corchorusii strain KN-1625 producing validamycin. The strain is deposited in the China Center for Type Culture Collection and has an accession number of CCTCC NO:M2015710. The strain can increase the content of validamycin A in a validamycin fermentation liquid, and can reduce the content of validamycin B. Compared with original strains, the content of the validamycin A when the strain is adopted is increased by 47%, and a ratio (B / A) of the content of the validamycin A to the content of the validamycin B is reduced by 37.56%. The validamycin yield is maintained to be higher than 30000 [mu]g / mL. The KN-1625 strain is good in hereditary stability. A validamycin level of the fourth generation is equivalent to that of the first generation.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a Jinggangmycin-producing Streptomyces corchorusii, and also relates to the application of the strain. Background technique [0002] Jinggangmycin is an agricultural antibiotic that can effectively prevent and control sheath blight. It is an environmentally friendly pesticide with "high control efficiency, no harm, and no pollution". It is the agricultural antibiotic with the largest output and use area today. Jinggangmycin belongs to aminoglycosides, mainly composed of A, B, C, D, E, F and other components, and later found components such as G and H, but only component A has the strongest control effect on sheath blight, High-content Jinggangmycin A can not only be used as a pesticide, but also can be used as an intermediate in the production of medicine, and its application prospect is very broad. However, the Jinggangmycin impurity content produced by microbial fermen...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12P19/46C12R1/465
CPCC12P19/46C12N1/205C12R2001/465
Inventor 夏建荣程治国王梦婕周莉邹维龚永华万俊金海燕
Owner WUHAN KERNEL BIO-TECH CO LTD
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