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Preparation method of raw validamycin powder

A technology of Jinggangmycin and raw powder, which is applied in the field of preparation of Jinggangmycin raw powder, can solve the problems of decreased permeability, limited application of ultrafiltration, concentration polarization, etc., and achieves simple steps, short production cycle, increased powerful effect

Active Publication Date: 2014-09-03
WUHAN KERNEL BIO-TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

During the ultrafiltration process, due to the continuous accumulation of trapped impurities such as amino acids, proteins, polysaccharides and macromolecular inorganic substances on the membrane surface, concentration polarization will occur. When the concentration of impurities on the membrane surface reaches a certain limit, condensation will be formed. The adhesive layer makes the permeability of the membrane drop sharply, which greatly limits the application of ultrafiltration

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] A preparation method of Jinggangmycin former powder, the method is: the Jinggangmycin fermented liquid is centrifuged at a high speed of 12000 rev / min to remove mycelium, the centrifuged liquid is first filtered with a ceramic microfiltration membrane with a pore size of 5 μm, and then In the filtrate, add 0.2% nano-silica by weight, 0.5% nano-alumina, 0.8% thermoplastic acrylic resin, 0.2% Tween 20, stir for 30 minutes, and the filtrate is adjusted to pH to 8, with molecular weight cut-off It is filtered by a hollow fiber ultrafiltration membrane of 15,000 Daltons, the pressure of the liquid inlet is 0.4MPa, the pressure of the liquid outlet is 0.2MPa, and the flow rate is 0.6m / s. The filtrate is collected, decolorized by activated carbon, concentrated under reduced pressure, and dried in vacuum. Instantly.

[0023] Detected by the method of CN103175906A, the Jinggangmycin A content in the Jinggangmycin raw powder obtained in this embodiment is 63.5%, and the Jinggangm...

Embodiment 2

[0026] A preparation method of Jinggangmycin, the method is: the Jinggangmycin fermented liquid is centrifuged at a high speed of 10000 rev / min to remove mycelia, the centrifugate is first filtered with a ceramic microfiltration membrane with a pore size of 1 μm, and then fed to the filtrate Add 0.1% nano-silica by weight, 0.8% nano-alumina, 0.5% thermoplastic acrylic resin, 0.1% Tween 20, stir for 10 minutes, and adjust the pH of the filtrate to 8.5, with a molecular weight cut-off of 20000 Dalton's hollow fiber ultrafiltration membrane filtration, the inlet pressure is 0.3MPa, the outlet pressure is 0.1MPa, the flow rate is 0.5m / s, the filtrate is collected, decolorized by activated carbon, concentrated under reduced pressure, and dried in vacuum to obtain .

[0027] Detected by the method of CN103175906A, the Jinggangmycin A content in the Jinggangmycin raw powder obtained in this embodiment is 60.8%, and the Jinggangmycin B content is 15.1%.

Embodiment 3

[0029] A preparation method of Jinggangmycin, the method is: the Jinggangmycin fermented liquid is centrifuged at a high speed of 12000 rev / min to remove mycelia, the centrifugate is first filtered with a ceramic microfiltration membrane with a pore size of 10 μm, and then fed to the filtrate Add 0.5% nano-silica by weight, 0.3% nano-alumina, 1% thermoplastic acrylic resin, 0.5% Tween 20, stir for 15 minutes, and adjust the pH of the filtrate to 7.5, with a molecular weight cut-off of 10000 Dalton's hollow fiber ultrafiltration membrane filtration, the inlet pressure is 0.5MPa, the outlet pressure is 0.3MPa, the flow rate is 0.8m / s, the filtrate is collected, decolorized by activated carbon, concentrated under reduced pressure, and vacuum dried to obtain .

[0030] Detected by the method of CN103175906A, the Jinggangmycin A content in the Jinggangmycin raw powder obtained in this embodiment is 58.4%, and the Jinggangmycin B content is 16.2%.

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Abstract

The invention discloses a preparation method of raw validamycin powder. The preparation method comprises the steps of centrifuging a validamycin fermentation solution at a high speed to remove mycelium; filtering the centrifugal liquid by using a ceramic micro-filtration membrane with the aperture of 1-10mu m; then, adding nanosilicon dioxide, nano aluminum oxide, thermoplastic acrylic resin and a surfactant with the HLB (Hydrophile-Lipophile Balance) value of 15-20 into the filtrate according to a certain weight, stirring for 10-30min, and regulating the pH value of the filtrate to 7.5-8.5; filtering by a hollow fiber ultrafiltration membrane of which the molecular cut off is 10000-20000 Dalton, collecting the filtrate, carrying out activated carbon decoloration, concentrating and drying to obtain the raw validamycin powder. The preparation method is capable of preparing the raw validamycin powder with high validamycin A and validamycin B contents, high in ultrafiltration efficiency and long in service life of the ultrafiltration membrane. In addition, the preparation method has the characteristics of simple steps, short production period, high efficiency and the like.

Description

technical field [0001] The invention relates to a preparation method of Jinggangmycin raw powder. Background technique [0002] Jinggangmycin is an efficient and safe agricultural antibiotic mainly used to prevent rice and wheat sheath blight. It is prepared by fermentation of Streptomyces hygroscopicus varietal Jinggang. Jinggangmycin is composed of A, B, C, D, E, F and other components, among which A component has the strongest activity, A and B exist at the same time to have a synergistic effect, while C, D, E, F and other components are almost No effect. [0003] At present, the Jinggangmycin raw powder produced in China is generally not separated and purified, but the fermentation liquid is simply filtered. The Jinggangmycin produced by this method has high impurity content, dark color, low active ingredient content, and Jinggangmycin A content Generally lower than 30%, only low-end agricultural products can be prepared. The Chinese patent of CN101497636A discloses a...

Claims

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Application Information

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IPC IPC(8): C07H15/22C07H1/06
Inventor 李青刘华梅杨克华刘荷梅程治国周莉邹维
Owner WUHAN KERNEL BIO-TECH CO LTD
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