Micro grafting method for test-tube plantlet of Catalpa fargesii Bur.f.duclouxii (Dobe) Gilmour and C.ovata G.Don

A technique of micro-grafting and Catalpa yunnanensis is applied in the field of seedling production of catalpa tree species, can solve the problems such as the scion and the rootstock are not easily fixed, the cambium is not easy to align, the grafting survival rate is low, etc., and the grafting survival rate is high and the operation is meticulous. , the effect of small footprint

Pending Publication Date: 2017-05-31
HUAZHONG AGRI UNIV +1
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Problems solved by technology

[0007] In addition, in order to solve the problem of using tinfoil as the binding and fixing material for the scion and rootstock in the micro-grafting of plant test-tube seedlings, it requires careful operation, takes a long time, and the scion and rootstock are not easy to fix, the cambium is not easy to align, and the survival rate of grafting is low. , the present invention uses the transparent silicone tube as the binding and fixing material for the scion and the rootstock when carrying out the micro-grafting of the tissue cultured seedlings of Catalpa and Catalpa. In addition to being not limited by the seasonal environment, it occupies a small area and has a high reproduction coefficient. In addition to the general advantages of micro-grafting of test-tube seedlings such as simple management and fast seedling growth, it also has the advantages of simple and convenient operation, short time-consuming, easy fixing of scion and rootstock, easy alignment of cambium, and high grafting survival rate. It is very suitable for Seedling production of Catalpa cumulus

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[0032] In order to make the object, technical solution and advantages of the present invention clearer, various embodiments of the present invention will be described in detail below in conjunction with the accompanying drawings. However, those of ordinary skill in the art can understand that, in each implementation manner of the present invention, many technical details are provided for readers to better understand the present application. However, even without these technical details and various changes and modifications based on the following implementation modes, the technical solution claimed in each claim of the present application can be realized.

[0033] 1. Grafting material preparation steps:

[0034] (1) Material selection: select excellent individual plants of Catalpa diaspora as scion materials, and excellent individual plants of Catalpa as rootstock materials.

[0035] (2) Preparation of primary medium: DKW (Driver and Kuniyuki Walnut) was selected as the basic ...

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Abstract

The invention discloses a micro grafting method for test-tube plantlets of Catalpa fargesii Bur.f.duclouxii (Dobe) Gilmour and C.ovata G.Don. According to the micro grafting method, when micro grafting of tissue culture seedlings of Catalpa fargesii Bur.f.duclouxii (Dobe) Gilmour and C.ovata G.Don is implemented, a transparent silica gel tube is adopted as a binding and fixing material of a scion and a stock, so that besides common advantages of test-tube plantlet micro grafting that the micro grafting is not limited by seasons or environments, a small floor space is needed, the propagation coefficient is high, final-period management of a grafted seedling is simple, seedlings can be produced rapidly, the micro grafting method further has the advantages of being simple and convenient to operate, short in time, easy in scion and stock fixation, easy in formed layer alignment and high in grafting survival rate, and is applicable to plantlet production of Catalpa fargesii Bur.f.duclouxii (Dobe) Gilmour.

Description

technical field [0001] The invention relates to a micro-grafting method of test-tube seedlings of Chinese catalpa and catalpa, which is a fast and simple propagation method for grafting tissue-cultured seedlings of Chinese catalpa and catalpa by using a transparent silicone tube in a sterile environment, and belongs to catalpa. The technical field of seedling production of tree species. Background technique [0002] Catalpa fargesii (Catalpa fargesii Bur.f.duclouxii (Dode) Gilmour) is a tall deciduous tree in the Catalpa group of Bignonicaceae (Bignonicaceae). It is a precious high-quality timber and garden ornamental tree species in my country. "Wood King", its wood is tough and dense, not easy to be eaten by insects, wear-resistant, rot-resistant, and moisture-proof, and is widely used in construction, furniture, crafts and other industries. Due to the autogenous sterility of Catalpa dianthus, the amount of seeds set is very small, the germination rate of seeds is low, and...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00A01G1/06
CPCA01G2/30A01G2003/005A01H4/008
Inventor 杜克兵张烨然张新叶李振芳陈慧玲杨杉
Owner HUAZHONG AGRI UNIV
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