A kind of test tube micro-grafting method of watermelon tissue culture seedling

Abstract

The invention discloses a watermelon tissue culture seedling test tube micro-grafting method. The method includes the steps of stock preparation, scion preparation, grafting, grafted body culture and grafted seedling transplanting. Aseptically germinated watermelons or gourds or pumpkin seedlings serve as stocks, seedless watermelon tissue culture seedlings serve as scions, grafting is conducted through a cleft grafting method, grafted bodies are placed in a solid medium for culture, and survived grafted seedlings are domesticated and then transplanted in a nursing medium, transplanted to a field in good time, and managed in a conventional cultivation mode. Cotyledons and growth points of the stocks are removed, lateral buds on the stocks do not need to be removed after the stocks are transplanted to the field, and therefore labor and time are saved. The tissue culture seedlings can be used for grafting when growing to be about 1cm, rooting is not needed, and time is saved. The method is feasible, easy and convenient to operate, and wide in application range, and the survival rate is high. The method can be conducted all the year, conditions are manually controlled, and the problem that the survival rate of transplanted watermelon tissue culture regenerated plants is low is solved. A new way is provided for application of a grafting technology, and a scientific research system is provided for grafting relevant theoretical research.

Description

technical field [0001] The invention relates to the technical field of grafting, and more specifically relates to a test tube micro-grafting method of watermelon tissue culture seedlings. The method is easy to implement, easy to operate, high in survival rate and wide in application range. It provides a new way for the application of grafting technology, and provides a scientific research system for the theoretical research of grafting. Background technique [0002] watermelon[ Citrullus Lanatus (Thunb.) Matsum. et Nakai] Cucurbitaceae, watermelon genus, annual vine herb. Originating in Africa, it is one of the five largest fruits in the world and occupies an important position in my country's national economy. Grafting is currently the only effective way to solve soil-borne diseases such as watermelon wilt, but the development process of grafts is complex, easily affected by environmental conditions, and lacks an ideal research system, resulting in a lag in theoretical r...

AI Technical Summary

Problems solved by technology

(3) The heritable variation produced by grafting can also be used for breeding. Although grafting is asexual reproduction in theory and will not cause changes in the traits of scion offspring, it has been found that some grafting can cause heritable variation

[0004] There is no report on the research on micro-grafting technology in test tubes on watermelon crops