Applications of rotenone in treating atherosclerosis
A technology of atherosclerosis and rotenone, applied in the new application field of rotenone in the preparation of drugs for treating atherosclerosis
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Embodiment 1
[0021] Example 1 Rotenone can reduce the area of cardiac outflow tract plaques in ApoE knockout mice
[0022] The ApoE-deficient mice fed with high-fat and high-cholesterol diet were divided into control group and rotenone treatment group, with 6 mice in each group. The mice in the treatment group were fed with 100ppm rotenone for 8 weeks. After the experiment, the cardiac outflow tract of the mice was taken, frozen and embedded, and sectioned. The frozen sections were stained with Oil Red O, and the plaque area of the cardiac outflow tract of the mice was counted. Such as figure 1 , 2 The outflow tract plaque area of the mice in the rotenone-treated group was smaller than that in the control group, with a statistical difference (p=0.0368).
Embodiment 2
[0023] Example 2 Rotenone can reduce the aortic plaque area of ApoE knockout mice
[0024] ApoE-deficient mice fed with high-fat and high-cholesterol diet were divided into a control group and a rotenone treatment group, with 3 mice in each group. The mice in the treatment group were fed with 100ppm rotenone for 8 weeks. After the experiment, the aorta of the mouse was taken, the fat and connective tissue around the aorta were stripped off, stained with Oil Red O, and the proportion of the stained plaque area of the aorta was counted. Such as image 3 , 4 As shown, the ratio of the aortic plaque area of the mice in the rotenone treatment group was smaller than that in the control group, with a statistical difference (p=0.0159).
Embodiment 3
[0025] Example 3 Rotenone can reduce macrophage scavenger receptor CD36
[0026] C57B6 mice were intraperitoneally injected with 3ml of 3% thioglycollate, and three days later, the peritoneal macrophages were inoculated into 6-well plates. After 4 hours of adherence, the fresh medium was changed, and 0.1μM rotenone was pretreated for 2 hours, and then added 50 μg / ml of oxidized low-density lipoprotein (ox-LDL), after 24 hours, extract cellular RNA, and detect the situation of macrophage scavenger receptor CD36 by Q-PCR, such as Figure 5 As shown, rotenone can reduce the induction of CD36 by ox-LDL, suggesting that it may reduce the formation of foam cells.
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