Sample preparation method for analysis samples of bamboo shoot flesh

Abstract

The invention discloses a sample preparation method for analysis samples of bamboo shoot flesh. The method comprises the following steps: selecting raw material, cleaning, removing roots, drilling for sampling, chopping, mixing, freezing, packaging, and refrigerating to obtain the analysis samples of the bamboo shoot flesh. According to the method provided by the invention, sample points with different aging degrees and different physiological activities can all be obtained, drawbacks of the existing sample preparation are overcome, the problems of heterogeneity of growth of the bamboo shoots, poor representativeness of the traditional sampling methods and large deviation of reliability of comparison between batches are solved, the representativeness and accuracy of asymmetrical bamboo shoot flesh samples are greatly improved, system errors are reduced, and the reliability and accuracy of the analysis results can be improved. The method plays a huge role in the biological analysis of bamboo shoots, and is particularly applicable to the analysis of nutrient composition of bamboo shoots, analysis of heavy metal contents, analysis of pesticide residues, analysis of antibiotic contents, postharvest physiological and biochemical analysis, DNA or RNA extraction analysis and sampling.

Description

technical field [0001] The invention belongs to the technical field of food analysis, and in particular relates to a sample preparation method of bamboo shoot meat analysis samples for chemical and biological analysis of bamboo shoots. Background technique [0002] my country is located in the center of the world's bamboo distribution, and the bamboo forest area accounts for about 30% of the world's total bamboo forest area. The edible part of bamboo is bamboo shoots, which has a history of more than 3,000 years in China. Among the bamboo species in my country, there are at least 200 kinds of edible bamboo shoots, which have become one of the main forest vegetables in my country. With the progress of human civilization, during the Western Han Dynasty, bamboo shoots developed from personal use to commodity. The medicinal value and health benefits of bamboo shoots are widely recognized among the Chinese people. Traditional Chinese medicine believes that bamboo shoots are swe...

AI Technical Summary

Problems solved by technology

The previous sampling methods are all sampling methods with uniform texture, such as halved, quartered, diagonally divided, etc., which are applied to irregular samples, and the systematic error introduced is relatively large; the main problem of this sampling method It is: First, because bamboo shoots are different from traditional vegetables and fruits, they belong to buds in terms of plant biological organ classification, and they are relatively large shoots with particularly vigorous life activities. Different regions on the entire shoot are different. The overall bamboo shoot meat tissue of the sprouts is not uniform, and it is difficult to achieve strong representativeness in sampling, and the systematic error brought by sampling factors is relatively large

Second, when adopting the traditional sampling technique---halving or quartering method, due to the irregular shape of the bamboo shoots, it is difficult to achieve the ideal equal division, and the sample does not have the characteristics of equal division

Third, although the heterogeneity can be overcome by using the whole bamboo shoot as a sample, the sample size is large, which brings difficulties to preservation on the one hand, and on the other hand, only a small part of it can be taken for specific analysis, so there is no real exclusion system error factor

Fourth, especially in cell research, DNA & RNA extraction, the amount of sample required is extremely small, and traditional sampling methods cannot be widely representative

[0006] With the development of science and technology and the enhancement of people's health concept, more attention is paid to the quality and safety of food, while the pollution trend of heavy metals and pesticides is still expanding, and the risk of traditional sampling methods is increasing.

Images