Sample preparation method for analysis samples of bamboo shoot flesh

A technique for bamboo shoots and bamboo shoots is applied in the field of sample preparation for analyzing samples of bamboo shoots and meat, which can solve the problems of difficult preservation, non-uniformity, large systematic errors, etc., so as to improve representativeness and accuracy, improve reliability and Accuracy, the effect of reducing systematic errors

Inactive Publication Date: 2017-05-31
国家林业和草原局竹子研究开发中心
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The previous sampling methods are all sampling methods with uniform texture, such as halved, quartered, diagonally divided, etc., which are applied to irregular samples, and the systematic error introduced is relatively large; the main problem of this sampling method It is: First, because bamboo shoots are different from traditional vegetables and fruits, they belong to buds in terms of plant biological organ classification, and they are relatively large shoots with particularly vigorous life activities. Different regions on the entire shoot are different. The overall bamboo shoot meat tissue of the sprouts is not uniform, and it is difficult to achieve strong representativeness in sampling, and the systematic error brought by sampling factors is relatively large
Second, when adopting the traditional sampling technique---halving or quartering method, due to the irregular shape of the bamboo shoots, it is difficult to achieve the ideal equal division, and the sample does not have the characteristics of equal division
Third, although the heterogeneity can be overcome by using the whole bamboo shoot as a sample, the sample size is large, which brings difficulties to preservation on the one hand, and on the other hand, only a small part of it can be taken for specific analysis, so there is no real exclusion system error factor
Fourth, especially in cell research, DNA & RNA extraction, the amount of sample required is extremely small, and traditional sampling methods cannot be widely representative
[0006] With the development of science and technology and the enhancement of people's health concept, more attention is paid to the quality and safety of food, while the pollution trend of heavy metals and pesticides is still expanding, and the risk of traditional sampling methods is increasing.

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  • Sample preparation method for analysis samples of bamboo shoot flesh
  • Sample preparation method for analysis samples of bamboo shoot flesh
  • Sample preparation method for analysis samples of bamboo shoot flesh

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0052] The preparation of samples for the evaluation and analysis of the nutritional quality of bamboo shoots is as follows:

[0053] 1. Selection of raw materials: Among the commercially available bamboo shoots, choose fresh bamboo shoots with no eroded spots of diseases and insect pests on the shoot body and no mechanical damage on the shoot body;

[0054] 2. Cleaning of raw materials Put the selected bamboo shoots in a pool for cleaning, first wash off the silt on the base and the surface of the bamboo shoot shells with tap water at 10°C to 20°C; then rinse with clean water. Cleaning is completed within 3 to 10 minutes;

[0055] 3. Remove the base. Put the cleaned bamboo shoots on a clean chopping board, and use a stainless steel knife to cut off the base to a thickness of 3mm;

[0056] 4. Punch sampling Use a cleaned and sterilized small hole puncher to insert the bamboo shoot meat tissue longitudinally from the base of the bamboo shoot. Take samples at 3 points at the ba...

Embodiment 2

[0063] For the preparation of samples for the analysis of heavy metal content and pesticide residues in moso bamboo shoots, the process is as follows:

[0064] 1. Selection of raw materials Dig bamboo shoots from the origin of moso bamboo shoots or purchase bamboo shoots from farmers’ markets, and then choose bamboo shoots that have no damage to the bamboo shoots and no mechanical damage except the incision as raw materials for sampling;

[0065] 2. Cleaning Put the selected raw materials in the cleaning basin, wash off the mud on the base and the surface of the bamboo shoot shells with room temperature (about 20°C) tap water; then rinse with distilled water. Cleaning time is controlled within 5-8 minutes;

[0066] 3. Remove the base. Put the cleaned bamboo shoots on the wooden chopping board, and cut off the base with a stainless steel knife to a thickness of 2.5mm;

[0067] 4. Punch sampling such as Figure 1b As shown, use a cleaned and sterilized puncher to insert the ba...

Embodiment 3

[0074] Preparation of green bamboo shoot meat physiological and biochemical index analysis samples, the specific process is as follows:

[0075] 1. Selection of raw materials for bamboo shoots. The initial raw materials are freshly excavated bamboo shoots from the green bamboo forest in Mabu Village, Pingyang, Wenzhou from July to September. The bamboo shoots are free from disease and pest erosion spots, no mechanical damage except the incision, and the bamboo shoots are shiny. bamboo shoots. In the later stage, in the fresh-keeping process, the bamboo shoots without mildew spots that are stored for a certain time interval are selected as the sampling raw material bamboo shoots;

[0076] 2. Wash the selected initial raw materials with tap water to remove the mud on the base and the surface of the bamboo shoot shells; then rinse with pure water. The temperature of the cleaning water is controlled at 20°C to 30°C, and the entire cleaning time is controlled within 10 minutes. P...

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Abstract

The invention discloses a sample preparation method for analysis samples of bamboo shoot flesh. The method comprises the following steps: selecting raw material, cleaning, removing roots, drilling for sampling, chopping, mixing, freezing, packaging, and refrigerating to obtain the analysis samples of the bamboo shoot flesh. According to the method provided by the invention, sample points with different aging degrees and different physiological activities can all be obtained, drawbacks of the existing sample preparation are overcome, the problems of heterogeneity of growth of the bamboo shoots, poor representativeness of the traditional sampling methods and large deviation of reliability of comparison between batches are solved, the representativeness and accuracy of asymmetrical bamboo shoot flesh samples are greatly improved, system errors are reduced, and the reliability and accuracy of the analysis results can be improved. The method plays a huge role in the biological analysis of bamboo shoots, and is particularly applicable to the analysis of nutrient composition of bamboo shoots, analysis of heavy metal contents, analysis of pesticide residues, analysis of antibiotic contents, postharvest physiological and biochemical analysis, DNA or RNA extraction analysis and sampling.

Description

technical field [0001] The invention belongs to the technical field of food analysis, and in particular relates to a sample preparation method of bamboo shoot meat analysis samples for chemical and biological analysis of bamboo shoots. Background technique [0002] my country is located in the center of the world's bamboo distribution, and the bamboo forest area accounts for about 30% of the world's total bamboo forest area. The edible part of bamboo is bamboo shoots, which has a history of more than 3,000 years in China. Among the bamboo species in my country, there are at least 200 kinds of edible bamboo shoots, which have become one of the main forest vegetables in my country. With the progress of human civilization, during the Western Han Dynasty, bamboo shoots developed from personal use to commodity. The medicinal value and health benefits of bamboo shoots are widely recognized among the Chinese people. Traditional Chinese medicine believes that bamboo shoots are swe...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N1/28
CPCG01N1/286
Inventor 白瑞华刘振勇高百龙傅建萍俞志相
Owner 国家林业和草原局竹子研究开发中心
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