Method and test strip for rapidly detecting people susceptible to avian flu and human influenza

Abstract

The invention discloses a method and test strip for rapidly detecting people susceptible to avian flu and human influenza. The test strip orderly comprises a sample pad, a gold labeled pad, a detection control zone and a water absorption pad for absorbing the excess liquid in a detected sample along a chromatography direction. The detection control zone orderly comprises a detection band and a quality control line. The gold labeled pad comprises colloidal gold labeled lectin MAL-II or SNA. The detection band comprises stable internal reference lectin which is not bonded to the lectin MAL-II or a stable internal reference lectin which is not bonded to the lectin SNA. One end of the sample pad of the test strip is inserted into a saliva sample or is contained by the oral cavity, after 10 to 15 minutes, the test strip is taken out and the color development states of the detection band and the quality control line are observed so that the detection result is obtained. The method and test strip realize non-invasive, safe and simple detection of people susceptible to avian flu and human influenza.

Description

technical field [0001] The invention relates to rapid detection of avian influenza and human influenza virus-susceptible populations based on test strip technology, and can also be developed into a rapid detection of avian influenza and human influenza virus-susceptible populations by a double lectin sandwich method based on enzyme plate detection. Background technique [0002] Lectins are non-immune-derived, non-enzymatically active sugar-binding proteins that can specifically recognize and bind to a specific sequence of sugar chains in monosaccharides or polysaccharides with a special structure. Up to now, more than 100 plant-derived lectins have been found in nature. Lectins have various applications: such as the diagnosis of malignant tumors and other diseases, the design of antibacterial and antiviral drugs, the design of "biological missiles", and the identification and analysis of sugar chain structures, etc. With the introduction of microarray technology into the fi...

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Problems solved by technology

[0011] Chinese patent "A detection tool for screening avian influenza and human influenza virus susceptible population" (publication number: CN103884834A, publication date: 2014.06.25) discloses the relationship between lectin MAL-II and avian influenza susceptibility and lectin The relationship between SNA and human influenza virus susceptibility, and pointed out that the expression level of α2-3 or α2-6 linked terminal sialic acid sugar chain structure binding to the corresponding lectin MAL-II or SNA in the saliva of susceptible people was significantly down-regulated, However, this patent is only applied to chip kits, and the simplicity, speed and cost of detection need to be improved

[0012] The application of test strip detection technology in disease diagnosis and detection is gradually increasing, and it has the characteristics of rapidity and low price. However, there are no reports on test strips and detection methods for detecting avian influenza and human influenza susceptible populations.

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