Rice variety Zhejiang 5 black-streaked dwarf virus resistant site qRBSDV17 and molecular marker method thereof

A technology for rice black-streaked dwarf and black-streaked dwarf disease, applied in the field of agricultural science, can solve the problems of poor repeatability, time-consuming and labor-intensive, and speed up the breeding process of disease-resistant varieties, so as to speed up the breeding process, clear the location, and facilitate identification Effect

Inactive Publication Date: 2017-06-13
WUXI NANLIGONG TECH DEV
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  • Abstract
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Problems solved by technology

[0009] The purpose of the present invention is to provide rice variety Zhejing No. 5 anti-black-streaked dwarf locus and its molecular Marking method, which can predict the resistance of plants to rice black-streaked dwarf disease, simplifies the selection method, and accelerates the breeding process of disease-resistant varieties

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  • Rice variety Zhejiang 5 black-streaked dwarf virus resistant site qRBSDV17 and molecular marker method thereof
  • Rice variety Zhejiang 5 black-streaked dwarf virus resistant site qRBSDV17 and molecular marker method thereof

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Embodiment Construction

[0031] The methods used in the following examples are conventional methods unless otherwise specified.

[0032] 1. Screening of resistance sources and F2 population construction of Zhejing 5 / Lianjing 3:

[0033] In 2013, a total of 251 micro-core rice varieties from 15 provinces were collected for field induction identification of black-streaked dwarf resistance. The results showed that there were significant differences in the resistance of rice varieties to black-streaked dwarf in different provinces. The japonica rice variety Zhejing No. 5 in Zhejiang has good resistance to black-streaked dwarf disease, and the incidence rate in the field is only 5%, which belongs to the high level of resistance (Southwest Agricultural Journal, 2014, 27 (6): 2365-2369). The rice black-streaked dwarf resistance source Zhejing 5 was used as the female parent, and the rice black-streaked dwarf high-susceptibility variety Lianjing 3 was used as the male parent to prepare a hybrid F1, and a F2 s...

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Abstract

The invention relates to a rice variety Zhejiang 5 black-streaked dwarf virus resistant site qRBSDV17 and a molecular marker method thereof. According to the rice variety IR24 black-streaked dwarf virus resistant site and the molecular marker method, the filial generation F2 population of a rice black-streaked dwarf virus resistant variety Zhejiang 5 and a susceptible variety Lianyungang 3 is adopted as a mapping population; the rice black-streaked dwarf virus resistance is identified by using a combined method of natural field identification and improved laodelphax striatellus antixenosis identification; linkage analysis is carried out on virus resistance identification results and SSR marker data to obtain two SSR markers, closely linked to rice black-streaked dwarf virus resistance major QTL, on the chromosome 1; the resistance of a plant to the rice black-streaked dwarf virus can be predicated by detecting banding pattern data of the primer markers on the chromosome 1. Therefore, the selecting efficiency of black-streaked dwarf virus resistant rice is greatly enhanced and the rice breeding progress is accelerated.

Description

technical field [0001] The invention relates to a locus resistant to black-streaked dwarf disease of rice variety Zhejing No. 5 and a molecular marker method thereof, and belongs to the field of agricultural science and technology. Background technique [0002] Rice black-streaked dwarf disease is a viral disease caused by Rice black-streaked dwarf virus (RBSDV), which is mainly transmitted by the small planthopper. There are 28 species of hosts including hybrid rice, conventional rice, barley, wheat, corn, millet, sorghum, kangaroo, bluegrass, barngrass, barnyardgrass, and matang. Rice black-streaked dwarf virus infects corn and causes corn rough dwarf disease, and infects wheat to cause wheat dwarf disease. The pathogenic forms, hosts and symptoms, vector insects and disease transmission characteristics of these diseases are very similar. Rice black-streaked dwarf disease can occur at the rice seedling stage, tillering stage, and jointing stage. Due to different infection...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/11C12Q1/68
Inventor 不公告发明人
Owner WUXI NANLIGONG TECH DEV
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